67 research outputs found
Coding sequence dependent mRNA decay regulation
No full textPost-transcriptional mRNA regulation shapes gene expression, yet the full extent of cis-regulatory sequences and how translation shapes the regulation of these elements is poorly understood. To identify conserved cis-regulatory elements shaping mRNA stability, I analyzed the features associated with differential mRNA decay in two vertebrate systems: steady-state HEK293 cells and developing zebrafish embryos. I found that strength of translation initiation (Kozak score), uORF content, codon optimality, AU-rich elements, microRNA binding sites, and ORF length function combinatorially to regulate mRNA stability. Surprisingly, machine learning analysis identified ORF length as the most important conserved feature regulating mRNA decay. I found that mRNAs with long ORFs have lower translation efficiency, thus potentially more ribosome depleted regions. To find factors involved in targeting long ORFs to decay, I used yeast knockout screen that revealed Upf1 as a factor to target “poorly translated/untranslated” mRNA regions of long ORFs. Using a massive parallel reporter assay (MPRA) I show that Upf1 binds poorly translated and untranslated ORFs which are associated with higher decay rate, including mRNAs with uORFs and those with exposed ORFs after stop codons. Additionally, Upf1 knockdown stabilizes mRNAs with long ORFs or containing uORFs. My study emphasizes Upf1’s converging role in surveilling mRNAs with exposed ORFs that are poorly translated including mRNAs with long ORFs, ORF-like 3’UTRs and mRNAs containing uORFs. I propose that Upf1 regulation of poorly/untranslated ORFs provides a unifying mechanism of surveillance in regulating mRNA stability and homeostasis in an EJC-independent NMD pathway that I term ORF Mediated Decay (OMD)
Exotic branes in Double Field Theory
No full textThe non-geometric Q- and R-monopole are shown to be a particular case of the DFT-monopole solution. The notion of magnetic charge for the solutions is defined and shown to be equal to the magnetic charge of the NS5-brane solution. This is a talk presented by the author at the conference QUARKS’16 in St.-Petersburg
Legal mechanisms of attracting foreign citizens to jobs in Uzbekistan: existing problems and their solutions
No full textIn this article, the author analyzes the legal mechanism of the process of recruiting foreign citizens in Uzbekistan, as well as regulatory legal acts in a relevant area and the problems of their application. In the conclusion of the article, the author offers suggestions on improving the process of recruiting foreign citizens in Uzbekistan
Legal mechanisms of attracting foreign citizens to jobs in Uzbekistan: existing problems and their solutions
No full textIn this article, the author analyzes the legal mechanism of the process of recruiting foreign citizens in Uzbekistan, as well as regulatory legal acts in a relevant area and the problems of their application. In the conclusion of the article, the author offers suggestions on improving the process of recruiting foreign citizens in Uzbekistan
Isometries of five-branes and T-duality
No full text© The Authors, published by EDP Sciences, 2018. T-duality chain starting from the NS5-brane is known to generate exotic backgrounds. These have been described as a DFT background whose orientation in the doubled space defines specific 10-dimensional solutions. The focus of this letter is at isometries of the corresponding background, their relation to the NS five-brane charge and the section constraint of DFT. This letter is based on the talk presented by the author at the conference QUARKS'18
Exotic branes in Double Field Theory
No full text© 2016 The Authors, published by EDP Sciences.The non-geometric Q- and R-monopole are shown to be a particular case of the DFT-monopole solution. The notion of magnetic charge for the solutions is defined and shown to be equal to the magnetic charge of the NS5-brane solution. This is a talk presented by the author at the conference QUARKS'16 in St.-Petersburg
Isometries of five-branes and T-duality
No full text© The Authors, published by EDP Sciences, 2018. T-duality chain starting from the NS5-brane is known to generate exotic backgrounds. These have been described as a DFT background whose orientation in the doubled space defines specific 10-dimensional solutions. The focus of this letter is at isometries of the corresponding background, their relation to the NS five-brane charge and the section constraint of DFT. This letter is based on the talk presented by the author at the conference QUARKS'18
Neon abundances in mercury-manganese stars: Radiative accelerators and non-LTE calculations
No full textWe make new non-local thermodynamic equilibrium calculations to deduce the abundances of neon from visible-region echelle spectra of selected Ne i lines in seven normal stars and 20 HgMn stars. We find that the best strong blend-free Ne line that can be used at the lower end of the effective temperature Teff range is λ6402, although several other potentially useful Ne i lines are found in the red region of the spectra of these stars. The mean neon abundance in the normal stars (log A=8.10) is in excellent agreement with the standard abundance of neon (8.08). However, in HgMn stars neon is almost universally underabundant, ranging from marginal deficits of 0.1–0.3 dex to underabundances of an order of magnitude or more. In many cases, the lines are so weak that only upper limits can be established. The most extreme example found is υ Her with an underabundance of at least 1.5 dex. These underabundances are qualitatively expected from radiative acceleration calculations, which show that Ne has a very small radiative acceleration in the photosphere, and that it is expected to undergo gravitational settling if the mixing processes are sufficiently weak and there is no strong stellar wind. According to theoretical predictions, the low Ne abundances place an important constraint on the intensity of such stellar winds, which must be less than 10−14 M⊙ yr−1 if they are non-turbulent
CAVITY RING DOWN SPECTROSCOPY OF CARBON CHAIN RADICALS
No full textT. Motylewski and H. Linnartz, Rev. Sci, Instrum. 70, 1305(1999). T. Motylewski, H. Linnartz, O. Vaizert, J.P. Maier, G.A. Galazutdinov, F.A. Musaev, J. Krelowski, G.A.H. Walker, and D.A. Bohlender, Astrophys. J. 531, xxxx(2000)Author Institution: Department of Physical Chemistry, University of BaselCavity ring down spectroscopy through a supersonic planar plasma is used to study the electronic transitions of a series of carbon chain radicals in direct The gas phase spectra of species of the form , , , and are compared to the hitherto reported diffuse interstellar band positions
Integrability properties of Motzkin polynomials
No full text© 2020 Author(s). We consider a Hamiltonian system that has its origin in a generalization of the exact renormalization group flow of matrix scalar field theory and describes a non-linear generalization of the shock-wave equation that is known to be integrable. Analyzing conserved currents of the system, this paper shows that these follow a nice pattern governed by coefficients of Motzkin polynomials, where each integral of motion corresponds to a path on a unit lattice
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