1,721,049 research outputs found
ANTI-LEU-19 IS A MARKER FOR NERVOUS-TISSUE IN THE MUCOSA OF THE HUMAN RECTUM
No full textDespite numerous investigations on the enteric nervous system, the knowledge about the mucosal innervation of the human rectum and colon is very shallow and mainly based on ultrastructural studies. We performed an enzyme (NADH-Ach) and immunohistochemical (NF, synaptophysin, anti-Leu-19) study on cryostat sections of the human rectal mucosa in order to study the possible additional characteristics of the mucosal innervation in depth. Enzyme histochemistry reveals positively staining fibers in the muscularis mucosae. Staining with antibodies against neurofilaments and synaptophysin reveals positively staining fibers in the lamina propria and muscularis mucosae. The latter staining is comparable with the findings obtained with anti-Leu-19. The monoclonal antibody anti-Leu-19 is characterized by a nerve-like staining in the mucosa revealed by nerve-like fibers from the plexus submucosus internus (Meissner) penetrating the muscularis mucosae, and creating a real plexus of fine twisted fibers in the lamina propria, around the crypts and underneath the epithelium. The distribution of the mucosal nerve-like pattern in the areas with mucosa-associated lymphoid follicles differs from the pattern observed in the lamina propria in between. Since the monoclonal antibody anti-Leu-19 and the monoclonal antibody NKH1a show a great similarity, we were interested also in the possible relation between the nerve like mucosal staining pattern and the lymphocytic population. Comparing the distribution pattern of anti-Leu-19 with other lymphocytic markers, there was an apparent similarity with the distribution of the T-helper subgroup. Our data indicate that the monoclonal antibody anti-Leu-19 shows a nerve-like staining pattern in the mucosa
THE INNERVATION OF THE LYMPHOID-TISSUE AT THE ILEOCOLONIC TRANSITION - AN ENZYME AND IMMUNOHISTOCHEMICAL STUDY
No full textUsing enzyme and immunohistochemical methods on whole-mount preparations and cryostat sections, a morphologic and semiquantitative study was performed of the nervous tissue in the appendix and the ileum (areas with and without Peyer's patches) of the rabbit. The plexus submucosus externus (Meissner) consists of a network of small ganglia, vaguely associated with the vascular submucosal plexus. From the nerve cell bodies, cell processes occasionally penetrate the lymphoid follicles at the junction between the mucosa and the submucosa while other extensions form a dense plexus in the lamina propria of the mucosa. No nerve fibers are present in the dome of the follicles. The plexus submucosus internus (Henle), consisting of large cell bodies and large processes, closely follows the blood vessels. The numeration of the nerve fibers of the submucosal plexus endorses the histological finding that the appendix is a richly innervated lymphoid organ. In addition, the plexus myentericus (Auerbach) of the appendix is a network of small meshes, while in the ileum, in the area of Peyer's patches, the same plexus is composed of a network with large meshes. These differences point to a higher density of innervation in the appendix. Yet a specialized anatomic distribution of the innervation of lymphoepithelial structures cannot be demonstrated
THE INNERVATION OF THE LYMPHOID-TISSUE AT THE ILEOCOLONIC TRANSITION - AN ENZYME AND IMMUNOHISTOCHEMICAL STUDY
No full textUsing enzyme and immunohistochemical methods on whole-mount preparations and cryostat sections, a morphologic and semiquantitative study was performed of the nervous tissue in the appendix and the ileum (areas with and without Peyer's patches) of the rabbit. The plexus submucosus externus (Meissner) consists of a network of small ganglia, vaguely associated with the vascular submucosal plexus. From the nerve cell bodies, cell processes occasionally penetrate the lymphoid follicles at the junction between the mucosa and the submucosa while other extensions form a dense plexus in the lamina propria of the mucosa. No nerve fibers are present in the dome of the follicles. The plexus submucosus internus (Henle), consisting of large cell bodies and large processes, closely follows the blood vessels. The numeration of the nerve fibers of the submucosal plexus endorses the histological finding that the appendix is a richly innervated lymphoid organ. In addition, the plexus myentericus (Auerbach) of the appendix is a network of small meshes, while in the ileum, in the area of Peyer's patches, the same plexus is composed of a network with large meshes. These differences point to a higher density of innervation in the appendix. Yet a specialized anatomic distribution of the innervation of lymphoepithelial structures cannot be demonstrated
ANTI-LEU-19 IS A MARKER FOR NERVOUS-TISSUE IN THE MUCOSA OF THE HUMAN RECTUM
No full textDespite numerous investigations on the enteric nervous system, the knowledge about the mucosal innervation of the human rectum and colon is very shallow and mainly based on ultrastructural studies. We performed an enzyme (NADH-Ach) and immunohistochemical (NF, synaptophysin, anti-Leu-19) study on cryostat sections of the human rectal mucosa in order to study the possible additional characteristics of the mucosal innervation in depth. Enzyme histochemistry reveals positively staining fibers in the muscularis mucosae. Staining with antibodies against neurofilaments and synaptophysin reveals positively staining fibers in the lamina propria and muscularis mucosae. The latter staining is comparable with the findings obtained with anti-Leu-19. The monoclonal antibody anti-Leu-19 is characterized by a nerve-like staining in the mucosa revealed by nerve-like fibers from the plexus submucosus internus (Meissner) penetrating the muscularis mucosae, and creating a real plexus of fine twisted fibers in the lamina propria, around the crypts and underneath the epithelium. The distribution of the mucosal nerve-like pattern in the areas with mucosa-associated lymphoid follicles differs from the pattern observed in the lamina propria in between. Since the monoclonal antibody anti-Leu-19 and the monoclonal antibody NKH1a show a great similarity, we were interested also in the possible relation between the nerve like mucosal staining pattern and the lymphocytic population. Comparing the distribution pattern of anti-Leu-19 with other lymphocytic markers, there was an apparent similarity with the distribution of the T-helper subgroup. Our data indicate that the monoclonal antibody anti-Leu-19 shows a nerve-like staining pattern in the mucosa
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Intestinal metaplasia is only predictive for intestinal type gastric cancer and not for lymphomas.
No full text- …
