1,720,958 research outputs found
Activation and platelets degranulation in the PRP (platelet-rich plasma): cytofluorimetric evaluation and kinetic analysis of TGF-β1 release through immunotest (ELISA)
No full textAim: The Platelet-Rich Plasma is a blood compound with high platelets density. By using immunotest ELISA. this study evaluates the activation and degranulation of the platelets during stocking procedure of the PRP, considering the role of the TGF-β1, a growth factor of the tissues, that is found in the α-platelet granules. Finally, to gain a better understanding of the quality and the quantity activationes state these datas are compared to the ones obtained with the cytofluorimetric analysis of the activation state of the
platelets in the PRP.
Methods: This study focuses on a sample of 19 patients (21-71 years-old), with no major deseases and waiting for an invasive oral-surgery. 24h before surgery each patient was sampled for 400ml of venous blood (+sodium citrate). Subsequently, the obtained blood sack was centrifugated to divided the erythrocytes from the plasma (900RPM, 13min, at 20°C). The plasma was then centrifugated (3300RPM, 15min) to respectively obtain the P.R.P. and the P.P.P. (Platelet- Poor Plasma). Thus, P.R.P. was then shaked to prevent platelet aggregation. Instead, from the P.P.P. were took the autologous-thrombin and cryoprecipitate. Finally, P.R.P. (5ml), cryoprecipitate (2ml), calcium-gluconate (1ml) and autologous-thrombin (2ml) were mixed in 10ml syringe; after gelification the compound was placed in the chirurgic site. Through test-ELISA were calculated the pg of TGF-β1 into P.R.P. and into compound after the gelification.
Results: The results for the TGF-β1 obtained through test-ELISA are the following. 1) For the diluited solution SN (1:300) is has been extimated a release after 1 hour (h) of 22366.50 pgTGFβ/ml, after 6h of 30527.50 pgTGFβ/ml, after 24h of 19138.88 pgTGFβ/ ml and in post gel of 11791.63 pgTGFβ/ml. 2) For the diluited solution (1:150) is has been extimated a release after 1h of 15712.33 pgTGFβ/ml, after 6h of 26850.00 pgTGFβ/ml, after 24h of 29450.00 pgTGFβ/ ml and in post gel of 15321,43 pgTGFβ/ml. The TGF-β1 percentage released has been calculated using two formulas: (SN/SN+TOT) e (SN/TOT). The results using first formula were respectively after 1h of 7.6%, after 6h of 8.6% and after 24h of 20.5%. The results using second formula were after 1h of 8.4%, after 6h of 14.8% and after 24h of 19.9%. These results have been matched with the ones obtained through cytofluorimetric analysis of the samples marked with monoclonal antibodies. They reveal the following datas: for CD 42 the percentage of exposure are after 1h of 79.1%, after 6h of 90.2% after 24h of 87.6% and in post gel of 11.9%; for CD 62p is after 1h of 29.1%, after 6h of 32.4%, after 24h of 52.7%; for CD 63 is after 1h of 64.5%, after 6h of 67.1%, after 24h of 80.3%.
Conclusion: From the cytofluorimetric analysis of the datas we state the procedure to produce P.R.P. it is satisfying since the gel has demostrated a high platelets density. When the activation and degranulation of the platelets rise (cytofluorimetric), the release of the TGF-β1 increases (ELISA), but only 20% of the total present in the platelets has been effectivly released in the first 24h. This implies that a great quantity of growth factors remains in the paltelets and is available in the chirurgic-site. Untill the 24h it has been observed an increasing of release of the TGF-β1, with a drop in the quantity once the gel was formed, bringing the levels close to the first hour
Comparative life cycle assessment of microalgae cultivation for non-energy purposes using different carbon dioxide sources
Full text linkThe ability of microalgae to sequester carbon and at the same time synthesise valuable compounds with potential applications in nutraceutical, pharmaceutical and cosmetic industries makes them attractive for commercial deployment, especially in view of a blue bioeconomy. Among microalgae, the diatom Phaeodactylum tricornutum is considered as an important potential source of omega-3 polyunsaturated fatty acids, such as eicosapentanoic acid, an essential polyunsaturated fatty acid with anti-inflammatory and antimicrobial properties. The aim of this study was to perform the Life Cycle Assessment of the cultivation of P. tricornutum - at semi-industrial scale in photobioreactor - for the production of high-quality bioactive compounds comparing synthetic carbon dioxide supply to a supply with waste carbon dioxide from a biogas upgrading process hypothesizing industrial symbiosis network. The effect of renewable energy use instead of the European electricity mix was also examined. Primary data on the production process, including the stages of cleaning and sterilisation, cultivation, harvesting and freeze-drying, were used. The midpoint impact categories recommended in the ILCD Handbook were used for performing the impact assessment. A sensitivity analysis was also performed on algal productivity, culture medium recirculation factor and amount of solvents per cleaning cycle. Firstly, results indicate in general cultivation and freeze-drying as the most contributing stages to the impacts. Secondly, they demonstrate in the comparative assessment that the use of carbon dioxide from the biogas upgrading is a feasible and attractive alternative to the synthetic one, as it allows for the improvement of the environmental performance of the production process in all the analysed impact categories. Finally, sensitivity analysis suggests that the environmental performance could be further improved by acting on other key factors, such as electricity source, nutrients culture medium and cleaning solutions
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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