1,721,052 research outputs found
Cytogenetic effect of thiabendazole and diphenylammine on cultured human lymphocytes: sister chromatid exchanges and cell cycle delay
No full textThe two fungicides analysed in this paper, Thiabendazole (TBZ) and Diphenylammine (DPA), are among the pesticides found in higher concentration in fruits and vegetables sold in Tuscany. These compounds were tested in "in vitro" lymphocyte cultures at different concentrations and using 3 protocols; protocol 1: the cultures were treated with the fungicides for 48 h; protocols 2 and 3: the cultures were treated with fungicides for 4 h in the presence or absence of the metabolic activator S9 mix. Both fungicides produced a slight increase in the SCE frequency in the 48 h treatment, at the higher non-toxic concentrations tested, but not when exposed for only 4 h, with or without S9 mix. As far as concerns the Proliferation Rate Index (i.e. the number of first, second and third mitoses), Thiabendazole also produced a significant decrease in the replication rate of the treated cultures, while Diphenylammine did not produce any effect
The effect of sucrose or starch-based diet on short-chain fatty acids and faecal microflora in rats.
No full textHydrocaffeic and p-coumaric acids, natural phenolic compounds, inhibit UV-B damage to WKD human conjunctival cells in vitro and rabbit eye in vivo
No full textThis paper studied the effect on UV-B ocular damage of 10microM hydrocaffeic acid (HCAF) alone and as a mixture (MIX) (5 microM HCAF+5 microM p-coumaric acid). Since ocular UV-B damage is mediated by reactive oxygen species, the aim was to test if HCAF and MIX could reduce oxidation damage in human conjunctival cells (WKD) in vitro and in cornea and sclera of rabbits in vivo. After UVB irradiation (44 J/m(2)) of WKD cells, 8-oxodG levels in DNA were markedly increased and this effect was attenuated by HCAF and MIX. Rabbit eyes were treated by application of HCAF and MIX drops before UV-B exposure (79 J/m(2)). Corneal and scleral DNA oxidation damage, xanthine-oxidase (XO) activity and malondialdehyde levels (MDA) in corneal tissue and prostaglandin E(2) (PGE(2)) in the aqueous humour were reduced by HCAF alone and in combination with p-coumaric acid, showing their potential as a topical treatment against UV-B damage
Taurine-calcium interaction measured by means of 13C-NMR
No full textDespite the fact that many of the physiological roles of taurine are still obscure, a series of experimental data have been published suggesting that taurine can have a function in the modulation of calcium fluxes at cardiac level [1-3] and at the level of liver mitochondria [4]. It was suggested that the interference of taurine with calcium fluxes could be interpreted as the result of the formation of a calcium-taurine complex [1-4]. This hypothesis was rejected in a paper of Igisu et al. [5]. We have further investigated this point by means of Fourier Transform 13C nuclear magnetic resonance (13C-FT-NMR). In recent years it was in fact demonstrated that this is a powerful tool for the study of weak ligand-metal interactions. [6]. © 1978
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