322,856 research outputs found
Effects of synbiotic intake on the gut microbiota analyzed by PCR-DGGE and Real-Time PCR
No full textThe human gastrointestinal tract harbours a diverse community of microorganisms which serve numerous important functions for the host wellbeing. Functional foods (pre-, pro- and symbiotics) are commonly used to modulate the composition of the gut microbiota contributing to the maintenance of health or prevention of disease. 16S rRNA-based techniques represent eligible approaches to investigate the biodiversity of the gut ecosystem and specific responses to diet supplements.
In the present study we evaluated the influence of a 30-days intake of a symbiotic food (0,5 g FOS, 10E9 CFU B. longum, 10E9 CFU L. helveticus) on faecal microbiota composition in 20 healthy volunteers. PCR-DGGE with universal primers for Eubacteria was employed to determine changes in the distribution of the dominant bacterial species. Cluster analysis of DGGE profiles showed higher inter-individual variation than that among the different time-points of the trial, suggesting a stabilising impact of the symbiotic food on the microbiota. Real-time PCR with B. longum and L. helveticus species-specific primers allowed to assess the intestinal colonization of the probiotic strains included in the symbiotics
Impact of culture conditions on the transcriptional levels of oxc gene and the oxalate consumption in Bifidobacterium
No full textOxalate is a highly toxic compound ubiquitous in the plant kingdom and widely consumed in human diet. A number of pathological conditions can arise from oxalate accumulation. Oxalate-degrading gut bacteria may play a key role in oxalate-related diseases by catabolizing free oxalate, reducing its plasmatic and urinary levels.
Thirteen Bifidobacterium strains were screened for their ability to degrade oxalate by a rapid and sensitive method based on capillary electrophoresis (CE). A 100% oxalate degrading capacity was observed only for B. animalis subsp. lactis, a species widely used in dairy and pharmaceutical probiotic products. Putative oxalyl-CoA decarboxylase (oxc) and predicted permease genes were identified in B. lactis BI07, one of the most active strains. B. lactis BI07 consumption of oxalate and carbon sources was evaluated by CE analysis of the culture broths recovered from batch fermentation processes carried out in different growth conditions. Transcriptional analysis, using reverse transcription-quantitative PCR, along with the kinetics of oxalate degradation revealed that acidic conditions were a fundamental prerequisite for oxc transcription and enzyme activity
SURGERY IN THE VERY OLD PATIENT - EVALUATION OF FACTORS LINKED TO POSTOPERATIVE MORBIDITY AND MORTALITY
No full textDevelopment of a high-performance affinity chromatography-based method to study the biological interaction between whole microorganisms and target proteins
No full textPropionic acid fermentation of glucose and glycerol by Propionibacterium acidipropionici ATCC 25562
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Relationships between NAD redox status and FAD degradation in S. cerevisiae mitochondria.
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Diffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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