1,721,005 research outputs found
Mesoporous silica particles functionalized with newly extracted fish oil (Omeg@Silica) inhibit lung cancer cell growth
No full textAim: To assess whether Omeg@Silica microparticles - fish oil from anchovy fillet leftovers (AnchoisOil) encapsulated within mesoporous silica particles - are effective in promoting antitumor effects in lung cancer cells. Methods: Three human non-small-cell lung cancer cell lines (A549, Colo 699 and SK-MES-1) were used. Cells were treated with AnchoisOil dispersed in ethanol (10 and 15 μg/ml) or encapsulated in silica and further formulated in aqueous ethanol. Cell cycle, reactive oxygen species, mitochondrial stress and long-term proliferation were assessed. Results & conclusion: Omeg@Silica microparticles were more effective than fish oil in increasing reactive oxygen species and mitochondrial damage, and in altering the cell cycle and reducing cell proliferation, in lung cancer cells. These in vitro antitumor effects of Omeg@Silica support its investigation in lung cancer therapy
Antiproliferative activity of Pyrrolo[3,2-c]quinoline derivatives
No full textThe pyrrolo[3,2-c]quinoline scaffold has been known as a core structure of a wide number of bioactive molecules. Several derivatives of such a tricyclic angular heterocycle have shown a wide spectrum of biological activities, such as hypotensive, anti-inflammatory properties, gastric (H)ATPase inhibition effect, and remarkable antitumor activity (1). The high therapeutic potentiality of such a skeleton along with our interest in targets featuring aza-polycondensed aromatic structures, attracted us to develop an alternative synthetic strategy, in order to reach a series of pyrrolo[3,2-c]quinolines in quantitative yields (2). The reaction of 3-acetyl-1,4-dione 1 with selected amines allowed the isolation of intermediates 2. Subsequent reduction of the nitro group and condensation with substituted aldehydes drove the cyclization to pyrrolo[3,2-c]quinoline ring system 3.Substituted pyrrolo[3,2-c]quinolines 3 were preliminary evaluated for antiproliferative activity (MTS assays) against 5 human tumor cell lines (colon tumor Caco2; breast cancer MCF7; brain cancer LAN5; cervix HeLa and lung tumor H292), and human epithelial bronchial cell line 16HBE as control.
Moreover, benzomethylen-dioxy derivative tested at DTP/NCI showed an appreciable and selective cell growth inhibitory effect against Leukemia SR, Melanoma MDA-MB-435, Renal Cancer UO-31 cell lines
Verso l’ottimizzazione dell’attività antiproliferativa di derivati pirroloisochinolinici
No full textEffects of small extracellular vesicles isolated from pleural effusion on lung cancer cell proliferation and migration
Full text linkPleural effusion (PE) is a common clinical manifestation associated with advanced stages of both malignant and non-malignant diseases. PE frequently occurs in advanced non-small cell lung cancer (NSCLC) and contributes to tumor progression. NSCLC accounts for more than 85% of the lung cancers and remains a problem worldwide due to its late diagnosis and low rate of response to treatment. Extracellular vesicles (EVs) present in PE are emerging as key mediators of intercellular communication, capable of transferring oncogenic signals through their molecular cargo. Among these molecules, microRNAs (miRNAs) are increasingly recognized as important drivers of cancer progression. miR-21 is a representative onco-miRNA, involved in lung cancer progression; moreover EV‐miR‐21 upregulation at the pre‐dissemination stage promotes cancer cell survival in the pleural cavity. This study compares, for the first time, the functional role of EVs isolated from malignant PE in NSCLC patients (NSCLC-PE-EVs) with those isolated from PE in patients with congestive heart failure (CHF-PE-EVs), focusing on their ability to modulate lung cancer cell behavior. The effects of these EVs were evaluated on COLO699 lung adenocarcinoma cells with proliferation, migration, and gene expression assays. NSCLC-PE was found to contain approximately twice the amount of EVs compared to CHF-PE. NSCLC-PE-EVs were enriched in the oncogenic miR-21-5p, while CHF-PE-EVs had higher levels of the tumor-suppressive miR-126-3p. Only NSCLC-PE-EVs induced dose-dependent increases in COLO699 cell proliferation and migration, consistent with elevated miR-21-5p expression. Functional studies confirmed that miR-21-5p mediates these effects by downregulating PTEN and PDCD4, and by upregulating MMP9 expression. Our findings show that NSCLC-PE-EVs promote malignant phenotypes in lung cancer cells via the transfer of miR-21-5p
(Bu3Sn)4TPPS and (Bu2Sn)2TPPS significantly inhibit the growth, migrationand tumorigenicity of human malignant melanoma cells
No full textMelanoma is one of the most aggressive and treatment-resistant human cancers, it is responsible worldwide
for the 80% of skin cancer related deaths which is largely due to its propensity to metastasize to other organs.
The growing understanding of the pathways involved in melanoma progression and development has led to
the identification of some interesting new molecules, however, the treatment options for metastatic
melanoma remain limited. Indeed, the key step in metastasis development is the tumour cell invasion of the
nearby host tissue and the journey powerfully depends on the detachment of single tumour cells from the
primary tumour. Therefore, the ability to block the migratory and invasive capacity of tumour cells offers a
new approach to treating patients with malignant disease. In this contest, the aim of our work was to
understand the consequences on melanoma metastatic progression and migration of the treatment with very
low concentrations of two organotin(IV) complexes of the meso-tetra(4-sulfonatophenyl)porphine, the
(Bu3Sn)4TPPS and the (Bu2Sn)2TPPS. In particular, in treated melanoma cell lines we showed an increase
of cell cycle arrest at G0/G1 or G2/M phase and the inhibition of cell colony formation. Notably, the
(Bu3Sn)4TPPS and (Bu2Sn)2TPPS treatment of melanoma cells decreases the expression and activation of
FAK, the expression of BRAF, HLA-DR and STAT3 as well as the cell migration through Boyden chambers
with differential media compartmentalization. The results obtained, suggested that (Bu3Sn)4TPPS and
(Bu2Sn)2TPPS could be used as adjuvant therapeutic agents for their role in the regression of melanoma
motility and metastatic disseminatio
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Apoptotic induction on HeLa tumor cell line. A comparison of activity between pyrazolo[1,2-a]benzo[1,2,3,4]tetrazinone derivatives and their synthetic precursors
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