1,721,018 research outputs found
Molecular epidemiological investigation of multidrug-resistant Acinetobacter baumannii strains in four Mediterranean countries with a multilocus sequence typing scheme
No full textThirty-five multidrug-resistant Acinetobacter baumannii strains, representative of 28 outbreaks involving 484 patients from 20 hospitals in Greece, Italy, Lebanon and Turkey from 1999 to 2009, were analysed by multilocus sequence typing. Sequence type (ST)2, ST1, ST25, ST78 and ST20 caused 12, four, three, three and two outbreaks involving 227, 93, 62, 62 and 31 patients, respectively. The genes bla oxa-58, bla oxa-23 and bla oxa-72 were found in 27, two and one carbapenem-resistant strain, respectively. In conclusion, A. baumannii outbreaks were caused by the spread of a few strains
Clonal Outbreak f multidrug resistano Acinetobacter baumannii in a teaching hospital in Italy: genetic epidemiology and efficacious containment
No full textEffect of aspirin on cell proliferation and differentiation of colon adenocarcinoma Caco-2 cells.
No full textIndagini geofisiche in Val Cenischia
No full textBOLL. DEL MUSEO REGIONALE DI SCIENZE NATURALI DI TORIN
H. pylori g-glutamyltranspeptidase upregulates COX-2 and EGF-related peptides expression in human gastric cells.
No full textGastric mucosa responds to
Helicobacter pylori induced cell damage by increasing the expression of COX-2 and EGF-related peptides. We sought to investigate the bacterial virulence factor/s and the host
cellular pathways involved in the upregulation of
COX-2, HB-EGF and amphiregulin in MKN 28 and AGS
gastric mucosal cells.
H. pylori strain CCUG 17874 was grown in Brucella broth supplemented with 0.2% (2,6-dimethyl)-b-cyclodextrins. The soluble proteins released in the culture medium by the bacterium were fractionated by exclusion size and anion exchange
chromatography. A single peak retaining the ability to
upregulate COX-2 and HB-EGF mRNA and protein
expression was obtained. SDS-PAGE analysis of the
peak showed two peptides with an apparent molecular
weight of 38 and 22 kDa, which were identified
by automated Edman degradation analysis as the
N-terminal and C-terminal peptides of H. pylori
g-glutamyltranspeptidase respectively. Acivicin, a
selective g-glutamyltranspeptidase inhibitor, counteracted
H. pylori-induced upregulation of COX-2 and
EGF-related peptide mRNA expression. An
H. pylori isogenic mutant g-glutamyltranspeptidase-deficient
strain did not exert any effect on COX-2, HB-EGF and
amphiregulin mRNA expression. Blockade of phosphatidylinositol-
3 kinase and p38 kinase, but not MAP kinase kinase, inhibited
H. pylori g-glutamyltranspeptidase-induced upregulation of COX-2 and EGFrelated peptide mRNA expression
Helicobacter pylori gamma-glutamyltranspeptidase upregulates COX-2 and EGF-related peptide expression in human gastric cells.
No full textEffect of aspirin on cell proliferation and differentiation of colon adenocarcinoma Caco-2 cells.
No full textMolecular epidemiology of sequential outbreaks of Acinetobacter baumannii in an intensive care unit shows the emergence of carbapenem resistance.
No full textThe molecular epidemiology of multidrug-resistant Acinetobacter baumannii was investigated in the medicalsurgical
intensive care unit (ICU) of a university hospital in Italy during two window periods in which two
sequential A. baumannii epidemics occurred. Genotype analysis by pulsed-field gel electrophoresis (PFGE) of
A. baumannii isolates from 131 patients identified nine distinct PFGE patterns. Of these, PFGE clones B and
I predominated and occurred sequentially during the two epidemics. A. baumannii epidemic clones showed a
multidrug-resistant antibiotype, being clone B resistant to all antimicrobials tested except the carbapenems
and clone I resistant to all antimicrobials except ampicillin-sulbactam and gentamicin. Type 1 integrons of 2.5
and 2.2 kb were amplified from the chromosomal DNA of epidemic PFGE clones B and I, respectively, but not
from the chromosomal DNA of the nonepidemic clones. Nucleotide analysis of clone B integron identified four
gene cassettes: aacC1, which confers resistance to gentamicin; two open reading frames (ORFs) coding for
unknown products; and aadA1a, which confers resistance to spectinomycin and streptomycin. The integron of
clone I contained three gene cassettes: aacA4, which confers resistance to amikacin, netilmicin, and tobramycin;
an unknown ORF; and blaOXA-20, which codes for a class D -lactamase that confers resistance to
amoxicillin, ticarcillin, oxacillin, and cloxacillin. Also, the blaIMP allele was amplified from chromosomal DNA
of A. baumannii strains of PFGE type I. Class 1 integrons carrying antimicrobial resistance genes and blaIMP
allele in A. baumannii epidemic strains correlated with the high use rates of broad-spectrum cephalosporins,
carbapenems, and aminoglycosides in the ICU during the study period
Role of prostaglandin in the proliferation and differentiation of colon adenocarcinoma CaCo2 cells
No full textIdentification of Acinetobacter genomic species 13TU by sequence analysis of the 16S-23S rRNA gene spacer region.
No full text- …
