1,721,029 research outputs found
Native cattle breeds of Southern Italy: karyological profile.
- Italian typical products of animal origin are strictly linked to native breeds. Their protection requires control of their reproductive and productive abilities. Hence the need for karyological studies to identify subjects with chromosome abnormalities linked to hypofertility or sterility. We report the results of karyological analyses carried out from January 2008 to December 2008 on 145 cattle of native breeds (Agerolese, Cinisara, Modicana and Podolica) reared in Southern Italy so as to evaluate and characterize the presence of chromosome abnormalities in subjects with normal phenotypes.
Besides the 128 karyologically normal subjects (2n=60, XY and 2n=60, XX), 17 were carriers of rob (1;29) and one male was a carrier of cellular chimerism 2n=60, XX/XY. According to our data there is a high frequency of rob (1;29) in Cinisara and Podolica breeds while in Agerolese there was only one case of rob (1;29) and none in Modicana
An improved characterization of horse (Equus caballus, 2n=64) chromosomes by using replicating G and R banding patterns
L’impiego dell’analisi di immagine computerizzata nella elaborazione del cariotipo di equidi
Una tecnica immunoenzimatica su vetrino (IPST) per evidenziare EBNA in cellule portatrici
Clinical cytogenetics in river buffalo (Bubalus bubalis, 2n = 50): new cases.
Although several studies have been undertaken on
river buffalo chromosomes,the clinical
cytogenetics is relatively recent in this important
bovid. Indeed,only when speci¢c research
programs on females river buffaloes with reproductive
disturbances and a collaborative work
between cytogeneticists and veterinary practioners
were undertaken,significant results will be
obtained. In this study we summarize the results
of a cytogenetic investigation performed during
the last three years on females with reproductive
problems and two males free-martin. Peripheral
blood cultures were untreated and treated with
BrdU and H-33258 six hours before harvesting
to get normal and R-banded preparations,respectively.
Slides were treated for CBA and R-banding
techniques. Some specific cases were also treated
for FISH-technique. Upon 59 investigated
animals,of which 57 were females with reproductive
problems and 2 were males free-martin,
14 (24%) were carriers of sex chromosome
abnormalities.
The following abnormalities were found in the
females: (a) sex reversal syndrome (2n =50,XY)
in two animals; (b) X-monosomy (2n =49,X0)
in one animal; (c) X-trisomy (2n =51,XXX) in
one animal; (d) free-martinism (2n =50,XY/XX
with different frequencies of female and male cells
among the animals) in 6 females; (e) a pronounced
C-band polymorphism in the X-chromosomes of
two females. Both rectal palpation in all females
and direct observation of some reproductive
organs (after the animal’s mating) revealed evident
abnormalities in the internal reproductive organs
An improved characterization of horse (Equus caballus,2n=64) chromosomes by using replicating G and R banding patterns
Fluorescent G and C bands in mammalian chromosomes by using early BrdU incorporation simultaneous to methotrexate treatment
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