1,721,008 research outputs found
Active E-Learning by Doing with ALDO
Full text linkIt has been proved how teaching and learning educational processes can largely benefit from the application of ICT-based services within e-learning platforms, such as collaborative editing and advanced data visualizations. However, among state-of-the-art solutions, no one is able to tackle the problem in a comprehensive way. In this extended abstract, we discuss ALDO (Active e-Learning by DOing), a novel, advanced digital framework supporting integrated facilities for effective, active e-learning. ALDO includes an active repository for collecting, sharing, retrieving, and analyzing relevant materials, collaborative editing services, an e-learning platform, and advanced visualization tools to inspect the spatial and temporal dimension of specific data contexts. All such services and tools are made available to teachers/students through a dedicated Web portal. Although the present research was carried out within the H2020 Project DETECt (Detecting Transcultural Identity in European Popular Crime Narratives), by focusing on the specific data context of European crime narrative, the generality of the framework makes it suitable for any type of educational task. The design and creation of above tools and services, together with their uses, are presented and discussed through a series of real examples taken from DETECt
A recombinant cell line expressing a form of the Y13-259 anti-p21ras antibody which binds protein A and may be produced as ascites
No full textWe have recently reported the molecular cloning of the immunoglobulin genes encoding the variable regions of the rat anti-p21ras antibody, Y13-259. These genes were reassembled into expression vectors supplying DNA sequences encoding human gamma 1 and kappa constant domains, as well as the leader sequence for antibody secretion, thus yielding Hu-Y13-259, a secretory anti-p21ras antibody containing human constant regions. We now report the creation of a recombinant cell line, NS0/Hu-Y13-259/B6, secreting high levels of the Hu-Y13-259 Ig. The antigen specificity of this recombinant antibody was demonstrated to be identical to that of the parental Y13-259, i.e. the amino acid sequence 60-76 of the p21ras protein. Unlike the parental cell line, the recombinant cells could be grown as ascites in mice, allowing the production of large quantities of the protein A-binding Hu-Y13-259 antibody
Intracellular immunization: Expression of antibody domains in the cytoplasm and in the nucleus of mammalian cells
No full text
- …
