1,720,984 research outputs found
Further evidence for an endogenous digitalis-like compound in newborn and adult plasma detected by anti-ouaboin antiserum
No full textevidence for an endogenous ouabain-like immunoreactive factor in human newborn plasma coeluted with ouabain on HPLC
No full textA novel beneficial effect of liraglutide: the reduction in subclinical atherosclerosis in patients with type-2 diabetes.
No full textAutoantibodies directed against ribosomal P proteins: use of a multiple antigen peptide as the coating agent in ELISA.
No full textAutoantibodies directed against the ribosomal proteins P0, P1 and P2 (P proteins) are specific for systemic lupus erythematosus (SLE) and there are some evidences that they could be related to the neuropsychiatric manifestations of the disease. In this study, a multiple antigen peptide (MAP) carrying four copies of the C-terminal peptide (13 residues) of the P2 protein, which is a common epitope of the three P proteins, was prepared for use in an ELISA assay. It was employed to detect antibodies directed against the ribosomal P proteins in 102 SLE patients and the results were compared with those obtained using immunoblotting (IB). With this new ELISA, antiribosomal P protein antibodies were found in 15/102 SLE sera. These results correlated well with the results of IB. Furthermore, we confirmed that naturally occurring antiribosomal P protein antibodies are directed mainly against the epitope containing the C-terminal sequence and shared by the three P proteins. MAP appears to be an excellent coating agent for ELISA assays designed to detect anti-P antibodies. Further experiments showed the superiority of MAP, compared to the free peptide, in the detection of weakly positive sera. This ELISA can also be used for the serological follow-up of SLE patients
Novel anti-obesity drugs and plasma lipids
No full textObesity is a health problem of global, epidemic proportions and a major risk factor
for chronic diseases resulting in accelerated morbidity and mortality. Dyslipidemia
with a predominance of small dense LDL, impaired functionality of HDL particles, and
increased serum levels of remnant particles due to impaired clearance of triglyceriderich
lipoproteins significantly heightens cardiovascular events in obese subjects.
Pharmacotherapy in combination with lifestyle modification is the primary approach
to reduce obesity-related cardiovascular risk. Although there are several potential
anti-obesity drugs, orlistat is the only agent that remains available in the market.
Lorcaserin and Qsymia®, approved by the US FDA last year, and contrave with potential
approval in 2014, are new anti-obesity drugs with promising therapeutic effects.
Although these drugs can be associated with adverse side effects, these agents have
favorable effects on lipid profiles. However, the need for safer anti-obesity agents is
clear
Novel anti-obesity drugs and plasma lipids
No full textObesity is a health problem of global, epidemic proportions and a major risk factor for chronic diseases resulting in accelerated morbidity and mortality. Dyslipidemia with a predominance of small dense LDL, impaired functionality of HDL particles, and increased serum levels of remnant particles due to impaired clearance of triglyceride-rich lipoproteins significantly heightens cardiovascular events in obese subjects. Pharmacotherapy in combination with lifestyle modification is the primary approach to reduce obesity-related cardiovascular risk. Although there are several potential anti-obesity drugs, orlistat is the only agent that remains available in the market. Lorcaserin and Qsymia®, approved by the US FDA last year, and contrave with potential approval in 2014, are new anti-obesity drugs with promising therapeutic effects. Although these drugs can be associated with adverse side-effects, these agents have favorable effects on lipid profiles. However, the need for safer anti-obesity agents is clear. © 2014 Future Medicine Ltd
Proximal changes in signal transduction that modify CD8+ T cell responsiveness in vivo
Full text linkThe antigen dose conditions the functional properties of CD8+ T cells generated after priming.
At relatively low antigen doses, efficient memory T cells may be generated, while high
antigen doses lead to tolerance. To determine the mechanisms leading to such different
functional outcomes, we compared the proximal TCR signal transduction of naive cells, to
that of memory or high-dose tolerant cells generated in vivo. In vivo activation led to the constitutive
phosphorylation of CD3 4 , recruiting Zap70, in both memory and tolerant cells. In tolerant
cells, these phenomena were much more marked, the CD3 4 and ́ chains no longer
associated, and the Src kinases p56Lck and p59Fyn were inactive. Therefore, when the antigen
load overcomes the capacities of immune control, a new mechanism intervenes to block
signal transduction: the recruitment of Zap70 to CD3 4 becomes excessive, leading to TCR
complex destabilization, Src kinase dysfunction, and signal arrest
Effect of liraglutide on carotid intima-media thickness in patients with type-2 diabetes: a 4-month prospective study
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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