1,720,982 research outputs found
Fenomeni di oncosi controllata nelle piastrine durante la stabilizzazione del coagulo indotto da stimolo immunologico
Full text linkStimoli immunologici attivano piastrine umane, che vanno
incontro ad aggregazione e rilascio. Mentre alcune di queste risposte sono
state paragonate a fenomeni di apoptosi, altre, come la reazione di
rilascio e la stabilizzazione del coagulo, non sono caratteristiche di cellule
apoptotiche. In questo lavoro abbiamo voluto studiare l’effetto di uno
stimolo immunologico forte (zymosan opsonizzato) sulle piastrine.
Abbiamo studiato l’eventuale coinvolgimento dei mitocondri e delle
proteasi piastriniche. Lo studio dell’attività mitocondriale, effettuato
durante le fasi avanzate di attivazione piastrinica mediante metodo
citofluorimetrico con la determinazione della fluorescenza di JC-1, ha
evidenziato una completa perdita mitocondriale. Inaspettatamente, nello
stesso tempo la riduzione dell’MTT è risultata aumentata ed insensibile al
SOD e rotenone. Capsaicina e resinferotossina aumentano ulteriormente
la riduzione dell’MTT. L’analisi al Western Blotting non ha evidenziato
attivazione della caspasi, mentre vi era attivazione della calpaina. La
conclusione è che le piastrine, stimolate con zymosan opsonizzato, vanno
incontro a mitoptosi, ma viene mantenuta la vitalità cellulare grazie alla
attivazione di reduttasi extramitocondriali.Immunological stimuli activate human platelets, which
undergo aggregation and release reaction. While some of these responses
are been compared to phenomenons of apoptosis, others as release
reaction and clot stabilization, are not characteristic of apoptotic cells.
In this study we investigated the effect of a strong immunological
stimulus (opsonized zymosan) on the platelets. We have studied the
possible involvement of the mitocondria and the proteases of the
platelets. The study of the mitocondrial activity, performed during the
advanced phases of platelet activation through a cytofluorimetric method
by the determination of the JC-1 fluorescence, has underlined a complete
mitocondrial loss. Unexpectedly, the reduction of MTT results at the
same time increased in a SOD and rotenone insensitive way. Capsaicin and
resinferotoxin subsequently increase the reduction of MTT. The
Western Blotting analysis has not underlined caspase activation, while
there was calpain activation. In conclusion, platelets stimulated with
opsonized zymosan undergo mitoptosis, but maintain cellular viability due
to the extra-mitocondrial reductases
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Does oxidative stress play a critical role in cardiovascular complications of kawasaki disease?
No full textPretargeted antibody‐guided radioimmunotherapy in a child affected by resistant anaplastic large cell lymphoma
No full textAnaplastic large cell lymphoma (ALCL) is characterized by preferential paracortical and intrasinusoidal lymph node involvement by large anaplastic tumor cells expressing the CD30 antigen. Up to 80% of pediatric patients with ALCL can be cured with multi-agent chemotherapeutic regimens. Patients resistant to chemotherapy or suffering from early relapse have a poor prognosis and a poor chance of survival. In these cases, the highly aggressive clinical course of ALCL, associated with systemic symptoms and extranodal involvement, has been treated with different approaches in various cooperative trials, including conventional chemotherapy and human stem cell transplantation (HSCT). However, the optimal treatment has not yet been defined, in particular in cases of relapse. More recently, radioimmunotherapy has been studied with encouraging results in cancer patients, including non-Hodgkin's lymphoma. Here we describe the case of a pediatric ALCL, relapsing after HSCT, treated with pretargeted antibody-guided radioimmunotherapy, obtaining a complete remission, with excellent quality of life over the past 10 months. © 2007 The Authors
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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