1,720,983 research outputs found
A stress-related transcription factor belonging to the YL-1 family is differently regulated in durum wheat cultivars differing in drought sensitivity
No full textThe Mediterranean area is characterised by unfavorable environmental conditions such as heat stress and drought responsible for yield loss of crops like durum wheat, widely cultivated in this area. The response of plants to stressing environments is mediated by activation of a complex gene network, strictly related to the genetic background. Among the genes induced by drought, those coding for proteins acting as key regulators of signal transduction are of great interest. Characterization of these genes is a crucial point to understand their potential roles in plant stress response, also in view of their possible use in molecular breeding.
In this work we have characterised a Triticum durum gene, named TdDRG1, in two commercial cultivars, Primadur and Svevo, differing for drought stress resistance. TdDRG1 codes for a putative transcription factor belonging to the VPS72/YL-1 family, highly conserved in plants and animals. The expression analysis indicates that this gene is expressed at higher level in roots of the resistant cultivar Svevo, than in the susceptible Primadur. The gene structure was determined in both cultivars and the regulatory activity of 5′ upstream regions was analysed by transient expression analysis using tobacco protoplasts. Dissimilar expression level of TdDRG1 in the two cultivars can be explained by the differences observed in gene structure. In particular, differences in 5’ upstream regions could account for contrasting ability to cope with drought of the two cultivars. The data obtained in this study provide indications for further insight into the molecular basis of differences in drought stress response
A stress-related transcription factor belonging to the YL-1 family is differently regulated in durum wheat cultivars differing in drought sensitivity
No full textStudio dell’espressione di geni correlati con lo stress termico nell’ambito del progetto Si.Cer.Me.
No full text
Variabilità della tolleranza alla siccità e alle alte temperature in una collezione di frumenti tetraploidi
No full textAcquisition of thermotolerance and HSP gene expression in durum wheat (Triticum durum Desf.) cultivars
No full textPlants are strongly affected by heat stress, which they overcome by modifying several physiological and biochemical mechanisms. At the cellular and molecular levels, the synthesis of heat shock proteins (HSPs) is essential in preventing or minimising the deleterious effect of high temperature. Plant responses to high temperatures are mediated by both their inherent ability to survive known as basal thermotolerance, and their ability to acquire thermotolerance after acclimation. A major aspect of the acclimation response involves the expression of HSP genes. Different Triticum durum cultivars were characterised for their response to high temperature at the physiological and molecular levels. Determination of cell membrane stability for both basal and acquired thermotolerance, and HSP gene expression analysis were performed. The two genotypes which contrasted most in their ability to acquire thermotolerance were exposed to different stress conditions and the expression of HSP101C and four small HSP genes was analysed. Differences in HSP transcripts accumulation were observed during the acclimation treatments. There is substantial evidence that induction of HSP gene expression has a role in the acquisition of thermotolerance; moreover, the accumulation of mitochondrial HSP transcripts appears to be related to the acquisition of thermotolerance
Identification of a durum wheat gene coding for a putative member of the AGC protein kinase family
No full textStudio funzionale della regione promotore di un gene per HSP a basso peso molecolare di girasole
No full textStudio dell’espressione di geni per HSP in cultivar di grano duro (Triticum durum Desf.) con differente capacità di acquisire termo tolleranza
No full text- …
