1,720,964 research outputs found
The effects of Heavy metals exposition on the activity of recombinant plant plastidic glucose 6P dehydrogenase.
No full textChanges in the levels of HSP70 in relation to heavy metals exposition in Elodea canadensis .
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Glucose-6-phosphate dehydrogenase plays a central role in the response of tomato (Solanum lycopersicum) plants to short and long-term drought
No full textThe present study was undertaken to investigate the expression, occurrence and activity of glucose 6
phosphate dehydrogenase (G6PDH e EC 1.1.1.49), the key-enzyme of the Oxidative Pentose Phosphate
Pathway (OPPP), in tomato plants (Solanum lycopersicum cv. Red Setter) exposed to short- and long-term
drought stress.
For the first time, drought effects have been evaluated in plants under different growth conditions: in
hydroponic laboratory system, and in greenhouse pots under controlled conditions; and in open field, in
order to evaluate drought response in a representative agricultural environment.
Interestingly, changes observed appear strictly associated to the induction of well known stress
response mechanisms, such as the increase of proline synthesis, accumulation of chaperone Hsp70, and
ascorbate peroxidase.
Results show significant increase in total activity of G6PDH, and specifically in expression and
occurrence of cytosolic isoform (cy-G6PDH) in plants grown in any cultivation system upon drought.
Intriguingly, the results clearly suggest that abscissic acid (ABA) pathway and signaling cascade
(protein phosphatase 2C e PP2C) could be strictly related to increased G6PDH expression, occurrence and
activities.
We hypothesized for G6PDH a specific role as one of the main reductants’ suppliers to counteract the
effects of drought stress, in the light of converging evidences given by young and adult tomato plants
under stress of different duration and intensity
In-field study on traditional Italian tomato landraces: The constitutive activation of the ROS scavenging machinery reduces effects of drought stress
No full textThe involvement and the efficiency of the antioxidants scavenging system upon drought were examined
by comparing traditional tomato landraces with respect to an industrial commercial genotype (Red
Setter); for the first time, comprehensive analyses of physiological, biochemical and molecular parameters
were investigated directly under real field conditions, in a typical agricultural environment of
Southern Italy.
The characterization of the responses upon drought evidenced peculiar changes in stomatal conductance,
ascorbate peroxidase and catalase activities and expression in drought tolerant tomato landraces,
with respect to the industrial genotype.
An in silico analysis (promoter and co-expression study) coupled to a phylogenetic investigation of
selected enzymes was performed, reinforcing the hypothesis of a basal activation of ROS scavenging
machinery in the Mediterranean landraces.
Thus our data suggest a constitutive increase in the expression and activities of specific enzymes
involved in ROS detoxification that can play a pivotal role in the drought response shown by tomato
landraces.
Therefore, traditional landraces could represent an important source of useful genetic variability for
the improvement of commercial varieties; their ROS detoxifying capabilities denote peculiar aspects
worth being explored to better describe their specific stress tolerance
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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