1,721,043 research outputs found
Viral infection protocols
No full textThis chapter describes the protocol for preparation of recombinant adenoviruses and infection of target cells to express transiently G-protein-coupled receptors or other proteins of interest. Adenoviruses are nonenveloped viruses containing a linear double-stranded DNA genome. Their life cycle does not normally involve integration into the host genome, rather they replicate as episomal elements in the nucleus of the host cell and consequently there is no risk of insertional mutagenesis. The wild-type adenovirus genome is approx 35 kb, of which up to 30 kb can be replaced by foreign DNA. Adenoviral vectors are very efficient at transducing the gene of interest in target cells in vitro and in vivo and can be produced at high titers (>10(11)/mL). The viral infection has a number of useful features: (1) the efficiency of gene transduction is very high (up to 100% in sensitive cells). (2) The infection is easy and does not alter physically the cell membrane for gene transduction. (3) It is possible to infect cells that are resistant to transfection with plasmids (including nondividing cells)
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
P. I. E. N. O.—Petrol-Filling Itinerary Estimation aNd Optimization
Full text linkThe recent rise of intelligent transportation systems (ITS) has challenged the integration between different data sources. Reaching the goal of sustainable mobility requires properly managing and merging information coming from the vehicle (intra-) and information coming off the vehicle (inter-). In this paper, we provide a proof-of-concept leveraging on data merging between intra- and inter-networking presenting our framework: Petrol-Filling Itinerary Estimation aNd Optimization (PIENO). PIENO is a system that not only automates the search for the best fuel station but also paves the road to significant reductions in fuel consumption, making eco-driving a practical reality from a user perspective. The PIENO framework is designed to be fuel-type independent, ensuring its adaptability to different vehicles and conditions. It achieves this by merging data from the vehicle through a CAN Access Module (CAM) and data outside the vehicle through a mobile application connected to the internet. Different domains are stressed to reach the goal: microcontroller and OEM to retrieve the fuel level from the car, national authorities to retrieve the daily fuel price, AI models to predict the price trend for the next days, and algorithms to compute the best fuel station and the best time to fill. The modularity of PIENO allows it to adapt to different OEMs by modifying the intra-network interface to properly collect the fuel level, as well as to adapt to different markets and countries, retrieving the station’s locations and fuel prices by modifying the inter-network interface
G protein-coupled receptors: Heterologous regulation of homologous desensitization and its implications
No full textTwo patterns of rapid desensitization have been characterized for G protein-coupled receptors: homologous desensitization, which mainly involves G protein-coupled receptor kinases and arrestins, and heterologous desensitization, which mainly involves protein kinases A (PKA) and C (PKC). In this review, Tsu Tshen Chuang and colleagues discuss evidence to show that PKA and PKC can modify the functional state of the G protein-coupled receptor kinases/arrestin homologous desensitization machinery, providing a novel level of cross-talk in signal transduction. Studies on regulation of G protein-coupled receptor kinases and arrestins confirm that the functional state of this machinery may have important consequences for cellular responsiveness and may represent new targets for therapeutic strategies
Viral Infection for G Protein-Coupled Receptor Expression in Eukaryotic Cells
No full textThis chapter describes the protocol for the preparation of recombinant adenoviruses and infection of target cells to transiently express G protein-coupled receptors (GPCRs) or other proteins of interest. Adenoviruses are non-enveloped viruses containing a linear double-stranded DNA genome. Their life cycle does not normally involve integration into the host genome, rather they replicate as episomal elements in the nucleus of the host cell, and consequently there is no risk of insertional mutagenesis. Up to 30 kb out of the 35 kb of the wild-type adenovirus genome can be replaced by foreign DNA. Adenoviral vectors are very efficient in transducing target cells in vitro and in vivo and can be produced at high titers (>1011/ml). The viral infection has a number of useful features: (1) the efficiency of gene transduction is very high (up to 100 % in sensitive cells); (2) the infection is easy and does not physically alter the cell membrane for gene transduction; (3) it is possible to infect cells that are resistant to transfection with plasmids (including nondividing cells); and (4) the viral vectors can be used for infection in vivo (including gene therapy) and can potentially be targeted to specific cells or tissues
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Viral Infection for GPCR Expression in Eukaryotic Cells.
No full textThis chapter describes the protocol for the preparation of recombinant adenoviruses and infection of target cells to transiently express G protein-coupled receptors (GPCRs) or other proteins of interest. Adenoviruses are non-enveloped viruses containing a linear double-stranded DNA genome. Their life cycle does not normally involve integration into the host genome, rather they replicate as episomal -elements in the nucleus of the host cell, and consequently there is no risk of insertional mutagenesis. Up to 30 kb out of the 35 kb of the wild-type adenovirus genome can be replaced by foreign DNA. Adenoviral vectors are very efficient in transducing target cells in vitro and in vivo and can be produced at high titers (>1011/mL). The viral infection has a number of useful features: (1) the efficiency of gene transduction is very high (up to 100% in sensitive cells); (2) the infection is easy and does not physically alter the cell membrane for gene transduction; (3) it is possible to infect cells that are resistant to transfection with plasmids (including nondividing cells); and (4) the viral vectors can be used for infection in vivo (including gene therapy) and can potentially be targeted cell-specifically
Homovanillic acid and 5-hydroxyindoleacetic acid in human brain areas at autopsy. Effect of pre-death conditions with special attention to the state of consciousness and metabolic diseases
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