1,721,012 research outputs found

    CONCENTRAZIONI PLASMATICHE DI 15-CHETODIIDRO-PGF2alfa, CORTISOLO, PROGESTERONE E 17β-ESTRADIOLO NEL PERIPARTO DELLA CAPRA

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    L'obiettivo di questa ricerca era quello di valutare i profili ormonali durante periparto della capra. Lo studio è stato condotto su 31 capre gravide, di razza Camosciata delle Alpi. Gli animali sono stati sottoposti a due prelievi ematici giornalieri da 7 giorni prima a 7 giorni dopo il parto. La gravidanza si è conclusa con un parto spontaneo in 24 capre e con una distocia di diversa entità nelle restanti 7. Una capra è stata macellata in corso di parto per macrosomia fetale ed un’altra è stata ritrovata morta il giorno dopo il parto. Per quanto riguarda gli andamenti ormonali, si è registrato un andamento simile tra PGM, cortisolo (C) ed estradiolo 17 beta (E2), confermando quanto riportato in studi precedenti . Questi ormoni presentano infatti un significativo incremento preparto, con picco in corrispondenza del prelievo effettuato a -0.5 giorni; l’aumento dell’E2 ha inizio 2 giorni prima del parto, mentre i livelli di PGM e C si innalzano in modo significativo a partire dal giorno -1. Il successivo decremento risulta immediatamente significativo per C ed E2; il PGM invece, pur subendo un calo significativo 12 ore dopo il parto, rimane elevato per tutta la durata dello studio. Anche l’andamento del Progesterone appare sovrapponibile a quanto rilevato in studi precedenti; il trend negativo ha inizio alcuni giorni prima del parto, ma diviene significativo solo a partire da -2.5 giorni dal parto

    Reference Ranges for Hematological and Biochemical Profile of Martina Franca Donkeys

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    The Martina Franca donkey (MFd) is one of the largest Italian donkey breeds, considered as endangered breed. To support the conservation strategies, knowledge about the physiologic hematological parameters of MFds is needed. The aims of the study were to determine reference value for hematological and major serum parameters in a population of healthy MFds and to estimate the influence of age on these parameters. Eighty-one clinically healthy MFds (17 males and 64 females) in different ages were enrolled: group A (foals, n° 16, animals < 1 year old) group B (young animals, n° 36, from 1 to 3 years old), and group C (adult animals, n° 29, over 3 years old). Red blood cell count (RBC); hematocrit value (HCT); hemoglobin concentration (HGB); mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); hemoglobin concentration distribution width (HDW); RBC distribution width (RDW); total white blood cell (WBC); WBC differential count for neutrophils, lymphocytes, monocytes, eosinophils and basophils, and platelets (PLT); mean platelet volume (MPV); platelet volume distribution width; and plateletcrit (PCT) were analyzed. The biochemistry panel included aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), total serum protein (TP), albumin (ALB), cholesterol (CHOL), triglyceride (TGL), blood urea nitrogen (BUN), creatinine (CREA), glucose (GLU), Ca2+, phosphorus, Na+, Cl−, K+, and Mg2+. The effect of age on hematological parameters was investigated using one-way ANOVA test. Age of donkeys does not influence total WBC, HGB, HCT, platelet count and MPV, and PCT (P > 0.05). Some leukocyte populations such as eosinophils, monocytes, and basophils showed age-linked variations (P < 0.05). RBC count, RDW, and HDW decrease with age whereas MCV and MCH increase. Na+, K+, Cl−, Ca2+, phosphorus, ALP, GGT, CREA, GLUC, and CHOL decrease with age (P < 0.05), while AST and TP showed an increase with aging (P < 0.05). ALB reaches the lowest values in young donkeys and returns to values of foals in older animals (P < 0.05). Finally, a difference among groups for BUN and TGL was not found (P < 0.05). The results suggest how even for the MFd breed, age is a variable that affects different hematological and biochemical parameters. Compared to other donkey and horses, the MFd breed showed some differences that clinicians involved during conservation strategies need to be consider

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    PGF(2α), LH, testosterone, oestrone sulphate, and cortisol plasma concentrations around sexual stimulation in jackass.

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    Many hormones are involved in the regulation of male reproductive functions, controlling sexual behavior, and influencing sexual arousal, the onset of erection and ejaculation, and the post-ejaculatory detumescence. The aims of this study were to analyze the plasma concentrations of 15-ketodihydro-PGF(2α) (PGFM), LH, testosterone (T), oestrone sulphate (OS), and cortisol (C) in relation to sexual stimulation and to evaluate the possible correlations among circulating hormones and between hormones and semen characteristics in the donkey stallion. Thirteen sexually experienced Martina Franca jackass of proven fertility were enrolled and semen was collected through an artificial vagina. Plasma samples were collected at 12, 9, 6 and 3 min before oestrous jenny exposure, at the first erection in the mating arena in the presence of an oestrous jenny, during ejaculation, at dismounting, 3, 6, 9 and 12 min after ejaculation in box, and then every 10 min during the following 50 min. PGFM showed an increasing trend with significant differences between the pre-ejaculatory and post-ejaculatory period, suggesting a role of this hormone in the control of ejaculation. LH showed a significantly higher concentration at ejaculation compared to last samples, while T showed significantly higher levels at erection, ejaculation and dismounting, probably for its influence on these processes and on sexual behavior. Finally, OS did not show any difference in the period of observation, while C presented a significant increase only 22 minutes after erection. The only hormonal correlation found was a positive one between LH and T at erection and dismounting, while T and OS were positively correlated with total and progressive motility, respectively

    Plasma IGF-I concentrations in Holstein-Frisian heifers from birth to puberty.

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    The role of the IGF system is of particular importance in prenatal as well in postnatal growth and development. IGF-I is associated with increased somatic growth. The onset of puberty involves a complex interaction of pituitary, gonadal hormones and growth factors, such as IGF-I. The aim of the study was to study IGF-I plasma concentrations in Holstein-Friesian heifers from birth to puberty. The study was conducted on 6 female Holstein-Friesian (HF) heifers, born mature and viable. Blood samples were collected from the jugular vein into heparinized tubes, at 10 and 30 minutes (min), 3, 6, 12, 24 and 36 hours (h) after birth, at 7, 14, 21 and 28 days (d), every two weeks from 45 d to 105 d, and then monthly until after the occurrence of puberty, defined as the detection of a corpus luteum by rectal palpation. Separated plasma was stored until analyis by RIA for IGF-I. Possible IGF-I changes among each sampling time of study were statistically analysed by one way ANOVA, followed by the post Hoc Tukey test. The 6 heifer showed a normal post natal growth and development, and did not show diseases along the time of study. Because puberty occurred at different age among the 6 heifers (8-13 months of age), IGF-I plasma concentrations (means ± SD) profile from birth to puberty was subdivided in two separate figures, one reporting the profile of IGF-I levels from birth to 28 days of age (Figure 1), and another reporting IGF-I plasma levels from puberty back to 45 days of age (Figure 2). Results obtained in this study evidenced a significant (p<0.05) increase of IGF-I concentrations from birth to the time- frame between 10 min and the first 6 hours of age, probably due to the colostrums intake. Even if the colostral IGF-I are not absorbed by the intestinal mucosa, the elevated glucose status in colostrum-fed calves enhance the IGF-I hepatic synthesis resulting thus in elevated plasma IGF-I concentrations after colostrums intake [1]. From 7 to 28 days of age IGF-I return to values even lower to that recorded at birth, followed by a trend of increase from 45 days of age and with a significant (p<0.05) increases in the last 5 months before puberty, with high levels persisting until after puberty occurrence. In humans, IGF-I are reported to markedly increase during puberty with actions amplified mutually with sex steroids in the control of growth, muscle mass increases and skeletal mineralization [2]. 1) Hammon et al, 2012 Domest Anim Endocrinol 43, 171-85. 2) Mauras et al, 1996 Horm Res 45, 74-80. Endocrinologi

    Presentation of the “Parma Scale” for treatment evaluation in offenders with mental disorders

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    Routine treatment evaluation is still poorly implemented in the Italian forensic psychiatric practice. This is also a lack of reliable instruments, especially those sufficiently specific and sensitive to longitudinally measure behavioral and functional changes. Moreover, among the very few assessment tools specifically designed to investigate treatment progress of offenders with mental disorders and the changeability of their problematic/protective behaviors, the vast majority were developed in non-Italian countries and cultures. Therefore, given the high specificity of the Italian forensic psychiatric framework, the aim of this paper was to present a new Italian instrument, the “Parma Scale” for treatment evaluation of offenders with mental disorders, which was developed as a quick and easy forensic tool specifically designed for a dynamic assessment of treatment plans (supported by hope and oriented towards a functional, personal and social recovery) and for an accurate application in different forensic psychiatric settings
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