55 research outputs found
Contamination Controls for Analysis of Root Canal Samples by Molecular Methods : An Overlooked and Unsolved Problem
No full textIntroduction: It has been almost 20 years since molecular methods were first described for the analysis of root canal microbial flora. Contamination control samples are essential to establish DNA decontamination before taking root canal samples, and this review assessed those studies. Methods: Using PubMed, a search was conducted for studies using molecular microbial analysis for the investigation of endodontic samples. Studies were grouped according to the cleaning protocol, acquisition methods, and processing of control samples taken to check for contamination. Results: Of 136 studies applying molecular analysis to root canal samples, 21 studies performed surface cleaning and checking nucleotide decontamination with contamination control samples processed by polymerase chain reaction. Only 1 study described disinfection, sampling from the access cavity,, and processing by polymerase chain reaction and reported the result; that study reported that all samples contained contaminating bacterial DNA. Conclusions: Cleaning, disinfection, and checking for contamination are basic scientific prerequisites for this type of investigation; yet, this review identifies it as an overlooked issue. On the basis of this review, we call for improved scientific practice in this field.</p
Survival against the odds: microbiology of root canals associated with post-treatment disease
No full textDestroyed and Forgotten: The Biologische Versuchsanstalt and its Scientists
No full textThis article recounts for the first time the story of a scientific institute and its researchers, pioneering experimental biology in Vienna, following concepts in morphology formulated by Roux and Davenport. Founded in 1902 as a private research institute by a triumvirate of wealthy members of the Viennese bourgeoisie of Jewish descent, the Biologische Versuchsanstalt, better known as the Vivarium, executed a then novel interdisciplinary research program interconnecting biology, zoology, botany, physiology and physical chemistry. While presenting the biographies of the scientists, the author retraces a broad family based network of the cultural elite of Fin-de-Siecle Vienna. Reaching far beyond the boundaries of natural sciences he underlines the importance of this network for a better understanding of the socio-cultural and scientific setting in Vienna at the turn of the century. Apart from providing a first insight into the scientific achievements of the Vivarium and its leading members such as Przibram, Portheim, Figdor, Kammerer, the elder Pauli and Steinach, the author regards this institute as a significant example for a scientific institution destroyed by the Nazis in 1938 and consequently deleted from the collective memory of the scientific community. With his article the author hopes to stimulate further work on the institute.This article recounts for the first time the story of a scientific institute and its researchers, pioneering experimental biology in Vienna, following concepts in morphology formulated by Roux and Davenport. Founded in 1902 as a private research institute by a triumvirate of wealthy members of the Viennese bourgeoisie of Jewish descent, the Biologische Versuchsanstalt, better known as the Vivarium, executed a then novel interdisciplinary research program interconnecting biology, zoology, botany, physiology and physical chemistry. While presenting the biographies of the scientists, the author retraces a broad family based network of the cultural elite of Fin-de-Siecle Vienna. Reaching far beyond the boundaries of natural sciences he underlines the importance of this network for a better understanding of the socio-cultural and scientific setting in Vienna at the turn of the century. Apart from providing a first insight into the scientific achievements of the Vivarium and its leading members such as Przibram, Portheim, Figdor, Kammerer, the elder Pauli and Steinach, the author regards this institute as a significant example for a scientific institution destroyed by the Nazis in 1938 and consequently deleted from the collective memory of the scientific community. With his article the author hopes to stimulate further work on the institute
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