1,720,957 research outputs found
Induction of a multifactorial resistance phenotype by high paclitaxel selective pressure in a human ovarian carcinoma cell line
No full text
Biovailability of VEGF in tumor shed vesicles depends on vesicle burst induced by acidic pH
No full textTumor angiogenesis is regulated by a dynamic crosstalk
between tumor cells and the host microenvironment.
Because membrane vesicles shed by tumor cells
are known to mediate several tumor–host interactions,
we determined whether vesicles might also stimulate
angiogenesis. Vesicles shed by human ovarian carcinoma
cell linesCABAI and A2780 stimulated the motility
and invasiveness of endothelial cells in vitro. Enzymelinked
immunosorbent assay and Western blot analysis
revealed relevant amounts of vascular endothelial
growth factor (VEGF) and the two matrix metalloproteinases
MMP-2 and MMP-9, but not fibroblast growth
factor-2, contained in shed vesicles. An A2780 cell–
derived clone transfected to overexpress VEGF shed the
same amount of vesicles as did a control clone, but
contained significantly more VEGF within the vesicles.
Despite a greater amount of VEGF in vesicles of the overexpressing
clone, vesicles of both clones stimulated endothelial
cell motility to comparable levels, suggesting
that VEGF was stored within the vesicle and was unavailable.
Only following vesicle burst induced by acidic
pH (a characteristic of the tumormicroenvironment) was
VEGF released, leading to significantly higher stimulation
of cell motility. Thus, tumor-shed membrane vesicles
carry VEGF and release it in a bioactive form in
conditions typical of the tumor microenvironment
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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