473 research outputs found
Next-Generation Sequencing in Tumor Diagnosis and Treatment
No full textNext-Generation Sequencing (NGS) allows for the sequencing of multiple genes at a very high depth of coverage [...]
Special Issue: Next-Generation Sequencing in Tumor Diagnosis and Treatment II
No full textNext-generation sequencing (NGS) allows for the sequencing of multiple genes at a very high depth of coverage [...]
Thyroid-like metastases to the scalp from a papillary renal cell carcinoma: A case report
No full textThe skin can host metastatic tumors originating from different organs. We report a case of metastatic renal cell carcinoma to the scalp in a 73-year-old man with features very similar to those of thyroid papillary carcinoma. The histogenesis in relation to its structure is discussed
Should We Test Cancer Susceptibility Genes in Routinely Used Multigene Panels? A Case of Synchronous Lung Adenocarcinoma and Breast Cancer Associated With Germline CHEK2 Mutation
No full textNo abstract availabl
Extracutaneous Merkel cell carcinomas harbor polyomavirus DNA
No full textMerkel cell carcinoma is a neuroendocrine tumor, with characteristic morphological and immunohistochemical features. Originally reported as primary carcinoma of skin, it has been described in numerous other sites such as lymph nodes, oral cavity, breast, vaginal walls, and salivary glands. Recent studies have revealed in cutaneous Merkel cell carcinomas a clonally integrated polyomavirus, named Merkel cell polyomavirus. The aim of the present study was to verify the presence of Merkel cell polyomavirus in 5 cases of primary Merkel cell carcinomas of lymph nodes and 1 case of parotid gland to investigate similarities or differences among Merkel cell carcinomas from various sites. Cases studied were 5 primary Merkel cell carcinomas in lymph nodes, 1 in the parotid gland, and 12 in the skin. Twelve cases of primary and metastatic small cell carcinoma of the lung were also investigated. Immunohistochemistry for keratin 20, chromogranin, synaptophysin, and thyroid transcription factor 1 was performed in all cases. Viral DNA was studied using polymerase chain reaction assay and the products evaluated in agarose gel and sequenced. Cytokeratin 20 and Merkel cell polyomavirus were detected in all cases of primary Merkel cell carcinoma irrespective of their site of origin. On the contrary, all cases of pulmonary small cell carcinoma were negative for both Merkel cell polyomavirus and cytokeratin 20. It appears that cutaneous and extracutaneous Merkel cell carcinomas share similar histologic, immunohistochemical, and molecular features. This is further evidence that Merkel cell carcinomas are a multiorgan carcinoma and that Merkel cell polyomavirus might play a role in the pathogenesis of this neoplasm
A neurogenic model of adult brain cancer in Drosophila
No full textPrimary brain cancers are characterised by high cellular heterogeneity, with a subset of undifferentiated and highly tumourigenic cells responsible for cancer aggressiveness and relapse. Despite obvious anatomical differences between humans and flies, the structural and functional analogy of the respective nervous systems and the conservation of the cellular and molecular aberrations at the basis of the disease make Drosophila an excellent model for human brain cancer. Early inactivation of the tumour suppressor gene PTEN is frequent in primary glioblastoma, the most aggressive form of adult brain cancer whose origin is still controversial. This results in the inhibition of the polarity protein Lgl1 due to aPKC hyper-activation. Dysregulation of this molecular axis is sufficient to reprogramme human neural progenitors into cancer stem cells.
After having confirmed that the PTEN/aPKC/Lgl axis is conserved in Drosophila, we have disrupted it in type II neuroblasts, a cell population with a lineage comparable to that of mammalian neural stem cells, obtaining aggressive tumours that persist and keep growing in the adult leading the animals to premature death. This neurogenic model recapitulates many phenotypic traits of human brain cancers, included high proliferation rate, accumulation of undifferentiated neural cells, local invasiveness and genetic instability. Work in progress and future perspectives will also be presented
gene decreases anthracnose ontogenic resistance of unripe strawberry fruit stages
No full textRapid alkalinization factor (RALF) genes encode for ubiquitous small peptides that stimulate apoplastic alkalinization through interaction with malectin-like receptor kinase. RALF peptides may act as negative regulators of plant immune response, inhibiting the formation of the signal receptor complex for immune activation. Recently RALF homologues were identified in different fungal pathogen genomes contributing to host infection ability. Here, FaRALF-33-like gene expression was evaluated in strawberry fruits inoculated with Colletotrichum acutatum, Botrytis cinerea, or Penicillium expansum after 24 and 48 h post-infection. To investigate the role of FaRALF-33-like in strawberry susceptibility, transient transformation was used to overexpress it in white unripe fruits and silence it in red ripe fruits. Agroinfiltrated fruits were inoculated with C. acutatum and expression, and histological analysis of infection were performed. Silencing of FaRALF-33-like expression in C. acutatum-inoculated red fruits led to a delay in fruit colonization by the fungal pathogen, and infected tissues showed less penetrated infective hyphae than in wild-type fruits. In contrast, C. acutatum-inoculated white unripe fruits overexpressing the FaRALF-33-like gene decreased the ontogenic resistance of these fruits, leading to the appearance of disease symptoms and penetrated subcuticular hyphae, normally absent in white unripe fruits. The different response of transfected strawberry fruits to C. acutatum supports the hypothesis that the FaRALF-33-like gene plays an important role in the susceptibility of fruits to the fungal pathogen C. acutatum.Fil: Merino, Maria Cecilia. Universidad de Bologna; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guidarelli, Michela. Universidad de Bologna; ItaliaFil: Negrini, Francesca. Universidad de Bologna; ItaliaFil: De Biase, Dario. Universidad de Bologna; ItaliaFil: Pession, Annalisa. Universidad de Bologna; ItaliaFil: Baraldi, Elena. Universidad de Bologna; Itali
El lugar de la mujer en Platón y Aristóteles según Moller Okin
No full textEn Women in Western Political Thought, Susan Moller Okin busca mostrar, mediante el análisis de una serie de autores paradigmáticos del pensamiento político occidental, que la causa de que las mujeres continuemos siendo ciudadanas de segunda radica en que la mujer ha sido pensada casi siempre desde una perspectiva funcionalista: ninguno de los autores puede pensar a la mujer fuera del ámbito privado y como madres-esposas. Partiendo de esta hipótesis, este trabajo pretende reconstruir la lectura que la autora realiza de Platón y AristótelesIn Women in Western Political Thought, Susan Moller Okin attempts to demonstrate, by analysing a series of paradigmatic Western political thinkers, that what causes women to remain second-class citizens is the fact that they have ususally been seen from a functionalist mode of thought. Neither author is able to consider woman outside the private sphere and her role as wive/mother. From this starting hypothesis, this article intends to reconstruct Moller Okin?s reading of Plato and AristotleFil: Napoli, Magdalena Marisa. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación. Instituto de Investigaciones en Humanidades y Ciencias Sociales (UNLP-CONICET); Argentina.Fil: Di Biase, Luján. Universidad Nacional de La Plata
Scientific publications in gastroenterology and hepatology in Western Europe, USA and Japan in the years 1992-1996: A global survey
No full textAim: To evaluate number and quality of publications in
gastroenterology, hepatology and digestive endoscopy
from Western Europe (Belgium, Denmark, France, Germany,
Great Britain, Italy, the Netherlands, Spain, Sweden,
Switzerland), Japan and USA over a recent 5-year
period. Methods: We screened by computer for full liver/
gastrointestinal-related articles and reviews the top 40%
of journals (according to the annual rating of the SCI
Journal Citation Reports; Institute for Scientific Information
database) in most clinical and basic science disciplines
in the years 1992–1996. To be credited with an article,
a given country had to be the site of the first institution
where the work was conducted. Papers were rated
according to the impact factor of the Institute for Scientific
Information and to the ratio impact factor/mean
European impact factor. Data were also normalized for
nondefense research and development expenditure. Results
and Conclusions: As randomly tested, the computer
search had an error of B 5–10%. In Europe, Great Britain
achieved the highest total impact factor and the highest
number of papers. Most of the British impact factor
came from publications in British journals. The total USA
impact factor exceeded that of Europe by 20%. The average
impact factor for a single paper was highest for the
USA and, in Europe, for Germany. The temporal trend of
total impact factor showed Spain improving by 9% per
year, with Germany and Italy also displaying a substantial
growth. Expressed per funds allocated in nondefense
research and development, Great Britain and the USA
had the highest cumulative impact factor
Erratum to: Incidence of neuroepithelial primary brain tumors among adult population of Emilia-Romagna Region, Italy
No full textUnfortunately, some of the participants of the PERNO Study Group are missing in the original publication of the article. The correct details are given below: Participants: Agati R., Ambrosetto G., Bacci A., Baldin E., Baldrati A., Barbieri E., Bartolini S., Bellavista E., Bisulli F., Bonora E., Bunkheila F., Carelli V., Cerasoli S., Crisci M., Dall’Occa P., de Biase D., Ferro S., Franceschi C., Frezza G., GrassoV., Leonardi M., Marucci G., Morandi L., Mostacci B., Palandri G., Pasini E., Pastore Trossello M., Pession A., Poggi R., Riguzzi P., Rinaldi R., Rizzi S., Romeo G., Spagnolli F., Tinuper P., Trocino C., Visani M. (Bologna), Dall’Agata M., Faedi M., Frattarelli M., Gentili G., Giovannini A., Iorio P., Pasquini U., Galletti G., Guidi C., Neri W., Patuelli A.., Strumia S. (ForlÍ-Cesena), Casmiro M., Gamboni A., Rasi F. (Faenza, RA), Cruciani G. (Lugo, RA), Cenni P., Dazzi C., Guidi A.R., Zumaglini F. (Ravenna), Amadori A., Pasini G., Pasquinelli M., Pasquini E., Polselli A., Ravasio A., Viti B. (Rimini), Sintini M. (Cattolica, RN), Ariatti A., Bertolini F., Bigliardi G., Carpeggiani P., Cavalleri F., Meletti S., Nichelli P., Pettorelli E., Pinna G., Zunarelli E. (Modena), Artioli F., Bernardini I., Costa M., Greco G., Guerzoni R., Stucchi C. (Carpi, MO), Iaccarino C., Ragazzi M., Rizzi R., Zuccoli G. (Reggio Emilia), Api P., Cartei F., Fallica E., Granieri E., Latini F., Lelli G., Monetti C., Saletti A., Schivalocchi R., Seraceni S., Tola M.R., Urbini B. (Ferrara), Giorgi C., Montanari E. (Fidenza, PR), Cerasti D., Crafa P., Dascola I., Florindo I., Giombelli E., Mazza S., Ramponi V., Servadei F., Silini EM., Torelli P. (Parma), Immovilli P., Morelli N., Vanzo C. (Piacenza), Nobile C. (Padova)
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