1,720,971 research outputs found
Failure to detect Helicobacter pylori in nasal mucous in Helicobacter pylori positive dyspeptic patients.
No full textStomach biopsies and samples of nasal mucus were cultured in patients with dyspeptic symptoms who underwent endoscopy to evaluate the possible route of transmission of Helicobacter pylori (H pylori). 42 patients were examined. For each patient two biopsies from the stomach corpus and antrum were taken and, before endoscopy, one nasal swab was obtained. Biopsy samples were tested for urease test, microbiological culture, and histological examination. The nasal swab was processed for microbiological examination. H pylori was not found in the nasal mucus of any of the patients, including the 36 who had H pylori in gastric biopsies
TWELVE ABERRANT STRAINS OF STAPHYLOCOCCUS AUREUS SUBSP. AUREUS FROM CLINICAL SPECIMENS.
No full textA new biovar of Staphylococcus aureus subsp. aureus was isolated from human clinical specimens and
described on the basis of studies of 12 isolates that were compared with 11 standard reference strains. Both
DNA hybridization experiments and numerical taxonomy analysis demonstrated that these strains were strictly
related to S. aureus subsp. aureus; however, they were significantly different from the latter. The atypical
strains belonging to the new biovar can be distinguished from typical S. aureus subsp. aureus strains by their
cx-chymotrypsin, ca-glucosidase, P-N-acetylglucosaminidase, lipase (C-14), and leucine arylamidase enzymatic
activities and novobiocin resistance. Thus, the combination of a-glucosidase and 13-N-acetyl-glucosaminidase is
more useful for distinguishing these S. aureus strains from the other, typical ones
ORTHO-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE UREASE INDOLE BROTH, A NEW COMPOSITE TUBE MEDIUM FOR SALMONELLA SCREENING
No full textA new composite broth medium combining o-nitrophenyl-i-D-galactopyranoside (ONPG) and urease and
indole tests in a single tube is described. High-level agreement with individual conventional tests was recorded
in comparative studies with 2,412 cultures of members of the family Enterobacteriaceae, i.e., 100% agreement
with the exception of Hafnia spp. (96.3% agreement) for the ONPG test and Citrobacter, Enterobacter, and
Hafnia spp. (75, 86.4, and 98.2% agreement, respectively) for the urease test. The new medium seems
especially promising as a screen for Salmonella subgroup I which encompasses most pathogenic Salmonella
species other than the Arizona subgroup
In vitro activity of ciprofloxacin against aerobic bacteria isolated in a southern european hospital
No full text
T-MOD PATHWAY, A REDUCED SEQUENCE FOR IDENTIFICATION OF GRAM-NEGATIVE URINARY-TRACT PATHOGENS
Full text linkIn this paper, we describe a reduced sequence of identification that includes T-mod medium, a selective and
differential isolation medium which allows accurate presumptive identification of the most common gramnegative
bacteria encountered in urine samples. The present study, performed on bacteria isolated from 1,762
independent urine samples, has shown that a few selected tests (lysine and ornithine decarboxylase, urease and
trehalose fermentation tests) improve the identification accuracy of T-mod, making it possible both to identify
the less frequent species and to prevent some misidentifications of Klebsiella pneumoniae and Proteus mirabilis.
The proposed work flow agreed with conventional identification protocols to a 99.3% extent and allowed
identification of 87.4% of the isolates directly from the primary plate, 11.4% after 1 to 3 additional tests, and
1.2% after an identification gallery
NEW PLATE MEDIUM FOR SCREENING AND PRESUMPTIVE IDENTIFICATION OF GRAM-NEGATIVE URINARY-TRACT PATHOGENS
Full text linkA new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is
described. The medium combines adonitol fermentation, phenylalanine deaminase, and P-glucuronidase tests
and allows the indole and cytochrome oxidase tests to be performed directly from the plates. High-level
agreement with individual conventional tests was recorded in comparative studies with 504 cultures of
gram-negative rods. There was 100% agreement, except for the Providencia spp. indole spot test (61.6%
agreement). Adonitol fermentation by Providencia species could not be determined. Escherichia coli, Klebsiella
pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa were identified with a high efficiency (100, 85.7,
83.5, and 100% agreement, respectively) without further testing. There was 96% overall agreement for the 267
infected urine samples tested
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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