3,193 research outputs found
Living at Gele Scheikunde: How to redevelop former campus buildings by integrating social & ecological values?
No full textThis research looks at Gele Scheikunde - a TU Delft chemistry department building constructed in 1945. The complex served for educational purposes until 2012 and then was sold by TU for redevelopment.The ambition of the city of Delft is to increase the housing stock, furthermore, the number of TU campus users has grown significantly in recent years. This brings up a dilemma between densification and quality of life. For these reasons, abandoned or non-used buildings like TU faculty building Gele Scheikunde is envisioned for housing purpose. According to the official regulations and the Commission for Environmental Impact Assessment (MER), there is great potential in this university area to develop an innovative sustainable neighbourhood. Thus, the question that arises is: How to redevelop the former campus buildings?The goal is to research on how to cope with abandoned campus buildings by including social and ecological values into the transformation and re-adaption design. The approach for the research was to involve stakeholders in the co-creation process. This co-creation process was facilitated through the Minecraft workshop. During the workshop, not only experts (e.g. architects, designers, planners) but also external parties (e.g. neighbours, users, ecologists), decided over the built environment. However, a co-creation approach is not limited to one method. There are various ways to involve stakeholders: interviews, surveys, questionnaires and workshops, with or without heritage games. For this research, a combination of the above was chosen: interviews, surveys and a gaming workshop with Minecraft.This approach provided information on what is valued in the case study, why it is valued, what should be kept and what can be demolished. Besides all stakeholders shared their opinion on what would be the best “new program”.Gele ScheikundeHeritage4allArchitecture, Urbanism and Building Sciences | Heritage & Architectur
Strong biofilm production, antibiotic multi-resistance and high gelE expression in epidemic clones of Enterococcus faecalis from orthopaedic implant infections
No full textEnterococcus faecalis is an opportunistic pathogen, which today represents one of the leading aetiologic agents of nosocomial infections and, increasingly, of implant infections. Here, in a collection of 43 E. faecalis isolated from implant orthopaedic infections, virulence-related phenotypes (biofilm and gelatinase production) and genotypes (gelE and esp) were studied to characterize epidemic clones identified and grouped by ribotyping. The presence of the esp gene and a marked and steady biofilm formation ability appeared to be the features associated with the clonal spreading, as well as a conspicuous gelatinase production, whereas the simple presence of gelE appeared non-specific of the epidemic clones. Antibiotic multi-resistance and strong biofilm production abilities together with a high phenotypic expression of gelatinase are an important equipment of E. faecalis to colonize peri-prosthesis tissues and to spread out as causative agents of implant orthopaedic infections
Gele Scheikunde: Altering the existing
No full textThis project is about transforming existing buildings that are facing demolition. In opposition to the constant renewing of our physical world for better performing building, this graduation proposes a gradual mutation of a locality.Architecture, Urbanism and Building Sciences | Explorela
Gele Scheikunde: Altering the Existing
No full textThis project is about transforming existing buildings that are facing demolition. In opposition to the constant renewing of our physical world for better performing building, this graduation proposes a gradual mutation of a locality.Architecture, Urbanism and Building Sciences | Explorela
Figure 1 in Intestinal preservation in a birdlike dinosaur supports conservatism in digestive canal evolution among theropods
No full textFigure 1. Daurlong wangi holotype. (a), whole specimen. (b), skull. (c), detail of orbit region. (d), feather remains associated to the thoracic vertebrae. (e), anuran skeleton. Scale bars: 20 mm (b), 10 mm (c).Published as part of Wang, Xuri, Cau, Andrea, Guo, Bin, Ma, Feimin, Qing, Gele & Liu, Yichuan, 2022, Intestinal preservation in a birdlike dinosaur supports conservatism in digestive canal evolution among theropods, pp. 1-10 in Scientific Reports (19965) 12 (1) on page 2, DOI: 10.1038/s41598-022-24602-x, http://zenodo.org/record/736270
De broze gele trui: botstatus van wielrenners
No full textAlthough cycling has positive health effects, including improvement of cardiovascular fitness, it does not have a favourable effect on bone health. In professional cyclists, the bone mineral density (BMD) will actually have decreased at the end of an intensive season. As a professional cyclist's career progresses, there will be a further decrease in BMD particularly in the hip and lumbar spine. The forward leaning position, the repetitive movement at low intensity, and the fact that body weight is carried by the bicycle all contribute to non-stimulation of bone formation. The cyclists' low body weight, which is desirable for climbers in particular, and possibly their dietary pattern may exacerbate this negative effect. To reduce the risk of fractures both during and after a cycling career, it is desirable that more attention is paid to strengthening of the bones. The effectiveness of training programmes to strengthen the bones should be investigated in carefully designed research studies.RST/Applied Radiation & Isotope
Enterococcus faecalis metalloprotease compromises epithelial barrier and contributes to intestinal inflammation.
No full textMatrix metalloproteases (MMPs) mediate pathogenesis of chronic intestinal inflammation. We characterized the role of the gelatinase (GelE), a metalloprotease from Enterococcus faecalis, in the development of colitis in mice.Germ-free, interleukin-10-deficient (IL-10(-/-)) mice were monoassociated with the colitogenic E faecalis strain OG1RF and isogenic, GelE-mutant strains. Barrier function was determined by measuring E-cadherin expression, transepithelial electrical resistance (TER), and translocation of permeability markers in colonic epithelial cells and colon segments from IL-10(-/-) and TNF(?ARE/Wt) mice. GelE specificity was shown with the MMP inhibitor marimastat.Histologic analysis (score 0-4) of E faecalis monoassociated IL-10(-/-) mice revealed a significant reduction in colonic tissue inflammation in the absence of bacteria-derived GelE. We identified cleavage sites for GelE in the sequence of recombinant mouse E-cadherin, indicating that it might be degraded by GelE. Experiments with Ussing chambers and purified GelE revealed the loss of barrier function and extracellular E-cadherin in mice susceptible to intestinal inflammation (IL-10(-/-) and TNF(?ARE/Wt) mice) before inflammation developed. Colonic epithelial cells had reduced TER and increased translocation of permeability markers after stimulation with GelE from OG1RF or strains of E faecalis isolated from patients with Crohn's disease and ulcerative colitis.The metalloprotease GelE, produced by commensal strains of E faecalis, contributes to development of chronic intestinal inflammation in mice that are susceptible to intestinal inflammation (IL-10(-/-) and TNF(?ARE/Wt) mice) by impairing epithelial barrier integrity
The chromosomes of two Drosophila races: D. nasuta nasuta and D. n. albomicana
No full textHeterochromatin distribution and differentiation in metaphase chromosomes of two morphologically identical Drosophila races, D. nasuta nasuta and D. n. albomicana, have been studied by C- and N-banding methods. -The total heterochromatin values differ only slightly between these races. However, homologous chromosomes of the two Drosophila forms show striking differences in the size of heterochromatin regions and there is an alternating pattern in D. n. nasuta and D. n. albomicana of chromosomes which contain more, or respectively less heterochromatin than their counterparts in the other race. -Three different N-banding patterns could be obtained depending on the conditions of the method employed: One banding pattern occurs which corresponds to the C-banding pattern. Another pattern is the reverse of the C-band pattern; the euchromatic chromosome regions and the centromeres are stained whereas the pericentric heterochromatin regions remain unstained. In the Y chromosomes of both races and in chromosome 4 of D. n. albomicana, however, the heterochromatin is further differentiated. In the third N-banding pattern only the centromeres are deeply stained. Furthermore, between the races, subtle staining differences in the pericentric heterochromatin regions can be observed as verified in F1 hybrids. On the basis of C- and N-banding results specific aspects of chromosomal differences between D. n. nasuta and D. n. albomicana are discussed
Investigation of gelatinase gene expression and growth of Enterococcus faecalis clinical isolates in biofilm models
No full textObjectives: Enterococcus faecalis is the major reason for biofilm-related infections and it also interacts with Staphylococcus aureus in biofilms. Gelatinase (gelE) enzyme is an important virulence factor of E. faecalis for biofilm formation. This study aimed to compare the biofilm producing E. faecalis isolates from urine and urinary catheters. The influence of S. aureus on the growth of E. faecalis biofilm cells was also investigated in a dual biofilm model in vitro. Another aim was to evaluate E. faecalis gelE gene expression during biofilm formation. Materials and Methods: Firstly, crystal violet staining was used to measure the total biofilm biomass of the isolates. Secondly, plate counting was performed to determine the biofilm formation ability of E. faecalis isolates and the effect of S. aureus on E. faecalis biofilm formation. Finally, the gelE expression profile of the isolates was assessed by quantitative real time-polymerase chain reaction. Results: According to crystal violet staining and plate counting, all E. faecalis isolates were biofilm producers and the number of E. faecalis sessile cells increased in the presence of S. aureus. Among the 21 E. faecalis isolates, ten expressed high levels of the gelE gene, while eight of them had low expression profiles (p<0.05). Conclusion: When they grow together, S. aureus may give some advantages to E. faecalis such as increasing sessile cell growth. The expression of the gelE gene was not affected by E. faecalis biofilm formation of the isolates collected from the patients with urinary tract infections.WoSScopu
- …
