1,721,008 research outputs found
Mushroom Tyrosinase in Polyelectrolyte Multilayers as an Optical Biosensor for o-diphenols
No full textDetermination of phenolic derivatives is very important in medical, food and environmental samples
because of their relevant significance in health care and pollution monitoring. Tyrosinase-based biosensors
are promising tools for this purpose because of several advantages with respect to currently used
detection methods.
A key aspect in the development of a biosensor is the effective immobilization of the enzyme. In this
work, ordered tyrosinase films on an optical transparent support were immobilized by a “layer-by-layer”
(LbL) assembly, alternating the enzyme with the polycation polymer poly(dimethyldiallylammonium
chloride). As confirmed by UV–vis spectroscopy, the LbL deposition allowed a high loading of enzyme.
The immobilized tyrosinase functionality was proven and its kinetic parameters were spectrophotometrically
determined. The prepared biosensor was used to optically detect the o-diphenolic compound
l-3,4-dihydroxyphenyl-alanine (l-DOPA) and exhibited good repeatability and time stability. The sensing
properties of the system were studied by means of both absorption and fluorescence spectroscopy.
The bioassay based on the absorbance measurements gave a LOD of 23M and a linear response up to
350M. The bioassay based on the fluorescence measurements gave a LOD of 3Mand a linear response
in the range of tens of micromolar (the exact value depends on the number of mushroom tyrosinase
layers). Biosensor sensitivity could be modulated varying the number of the immobilized enzyme layers
Interaction between probiotics and intestinal human epithelial cells: adhesion and induction of immune-responsive genes.
No full textAntibiotic resistence genes delivered by lactic Acid Bacteria isolated from ready to eat (RTE) food
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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