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Ultrastructure of human cumulus oophorus: a transmission electron microscopic study on oviductal oocytes and fertilized eggs.
No full textAbstract
The aim of the present study was to assess the heterogeneity of cumulus cells that occurs in human cumuli associated with oviductal oocytes and fertilized eggs. Transmission electron microscopy was used to study the cumulus masses surrounding both unfertilized oocytes and fertilized eggs (a pronuclear and a 4-cell stage) recovered at different intervals after ovulation. The specimens were obtained by flushing the oviducts of normal cycling women who underwent surgical sterilization. The cumuli were expanded due to large and irregular intercellular spaces; small linear gap junctions were seen at cell contacts, whereas annular gap junctions were found only in the cytoplasm of some cells. Both types of junction were less abundant in fertilized specimens. Cells surrounding fertilized eggs projected numerous long, thin microvilli into the intercellular spaces. As a rule, the inner layer of the cumulus mass (corona cells) was composed of cells whose surface was relatively smooth. Cumulus cells showed oval nuclei with one or more nucleoli. The cytoplasm of most cells possessed abundant organelles typical of steroidosynthesis: (i) mitochondria with tubular or villiform cristae; (ii) a well-developed smooth endoplasmic reticulum; and (iii) electron dense lipid droplets often surrounded by a few concentric membranes of smooth endoplasmic reticulum and/or in close contact with microtubules and microfilaments. Microperoxisome-like structures were also present. After fertilization, an enhancement of the steroidosynthetic characteristics occurred in the outer layers of the cumulus mass, but not in the corona cells, which still appeared capable of protidosynthesis. Together, these morphological features support the hypothesis that the cumulus of oviductal oocytes and particularly of fertilized eggs, luteinizes like parietal granulosa cells, generating a steroid hormonal micro-environment in the oviduct which may affect fertilization and zygote segmentation. Cumulus cells showing spermiophagic activity, as well as activated macrophages, leukocytes and red blood cells, were also found in the cumulus mass. The macrophages may play a local role both by phagocytic activity and by modulating the steroid secretion of the neighbouring cumulus cells which occurs in the ovarian follicle and corpus luteum. In conclusion, the cumulus mass surrounding tubal oocytes and fertilized eggs appears to be a heterogeneous and dynamic system, in which the micro-environment for fertilization and early embryo development is provided by diverse cell populations in addition to the oviductal cells
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
STUDY OF TIME COURSE OF EGG RETENTION ACTIVITY IN RABBIT OVIDUCT
No full textChanges in egg retention activity of the oviduct were assessed during the first 5 days after HCG[human chorionic gonadotropin]induced ovulation in rabbits. Dextran microspheres were used as ovum surrogates. They were injected into the peritoneal cavity at 0, 10, 32, 41, 50, 64, 72 and 80 h following HCG. The distribution of ova and surrogates in the genital tract was assessed 24 h following surrogate injection in all groups and at 8 h following surrogate injection in animals injected 50 h after HCG. The distribution of microspheres injected up to 50 h after HCG was similar to that of eggs. Surrogates were not retained at the ampullary-isthmic junction (AIJ) shortly after passage of ova through this region. Spheres injected 38 h after ovulation reached the eggs at the proximal isthmus in 8 h or less. Surrogates injected at 64 h following HCG or later were retained at the AIJ, indicating resumption of retaining activity at this level. Egg passage through the AIJ is probably associated with a temporary reduction of the retaining activity lasting for approximately 16 h. Retaining activity at the AIJ is regained before eggs pass from the proximal isthmus into the uterus
EFFECTS OF DELAYED TRANSFER AND TREATMENT WITH ESTROGEN ON THE TRANSPORT OF MICROSPHERES BY THE RAT OVIDUCT
No full textStarch or dextran blue microspheres were transferred microsurgically to the influndibulum of the oviduct on Days 1, 2, or 3 of pregnancy of control and oestradiol-treated rats. The animals were killed a few hours to several days after transfer to assess the number and distribution of ova and microspheres in the tract. After transfer on Day 1 of pregnancy, microspheres and eggs crossed the ampullary-isthmic junction (AIJ) 18 h after ovulation. After transfer on Day 2 of pregnancy, more than 50% of microspheres were retained in the ampulla, indicating that the AIJ changes again 34 h after ovulation. Treatment with oestradiol did not advance the passage of eggs or microspheres across the AIJ but caused accelerated transport through the isthmus as soon as the eggs or microspheres reached this segment. Dextran blue microspheres were seen to move back and forth in the isthmus of control anaesthetized rats at a frequency of 5-6 times/min. Between 7 and 20 h after treatment with oestradiol the frequency of these movements was significantly augmented, indicating that increased frequency of contractions of the smooth muscle of the isthmus precedes and accompanies accelerated transport of ova through this segment
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