6,153 research outputs found
Attraversando il Sahel: Elena Dak in cammino con i nomadi
No full textThe essay intends to examine the most recent reportage written by travel writer Elena Dak, published by Corbaccio in 2016, dedicated to Peulh Wodaabe nomads, better known as Bororo. The experience of nomadism, firmly rejecting a ‘touristic’ idea of visit, is experienced and narrated by a woman’s voice as an exception that can facilitate
the contact with the customs and traditions of a migrant culture, featured by ritual traditions, social practices and dances of extraordinary significanc
Replication Data for: "Conjugal plasmid transfer in the plant rhizosphere in the One Health context"
No full textThe dataset contains data related to the publication by Riva et al. entitled "Conjugal plasmid transfer in the plant rhizosphere in the One Health context" published on Frontiers in Microbiology in 2024 (https://doi.org/10.3389/fmicb.2024.1457854). Dataset refers to the data about the ability of strain Klebsiella variicola EEF15 RIF-R to colonize the lettuce rhizosphere and leaf endosphere. Data refer to bacterial counts on selective plates. Spreadsheet and readme files are available
Malte sostenibili a base di loppa d'altoforno attivata alcalinamente: studio della durabilità e delle proprietà smart
No full textUrban consolidation centres and competition among logistics service providers in a Hotelling approach
No full textUrban cities are affected by negative externalities coming from urban mobility and goods distribution, such as air pollution and traffic congestion. In recent years, many European municipalities had introduced market-based measures, aiming at externalities’ internalisation, such as vehicle pollution charges (e.g., the Ecopass system in Milan, the Low Emission Zones in London, etc.) together with structural solutions which, however, end up adding complexity (i.e., transhipment, delivery times, handling costs, etc.) to urban supply chains, such as the introduction of urban consolidation centres (UCCs) to cover last mile deliveries. By using a simple Hotelling spatial model, we analyse the effect of semi-public UCCs on urban competition among logistics service providers (LSPs) assuming heterogeneous urban retailers with respect to demand elasticity to delivery times. We show that for increasing public shares/interests into UCCs' ownership (implying lower service costs), LSPs that apply for UCC-based services are likely to increase their market shares even if competition among LSPs is relatively low (i.e., high differentiation) and retailers' demand elasticity to delivery times is relatively high. From a normative perspective, our framework does confirm that pollution charges and semi-public UCCs are substitutes in order to promote a more environmental-friendly urban freight logistics
Encuentros con Elena Poniatowska
No full textThe author analyzes testimonial literature from the perspective of female literature through his meeting with Elena Poniatowska. An analysis of reality vs. Fiction in Elena, Jesusa and Tinisima.El autor analiza, desde su encuentro con Elena Poniatowska, la vertiente de la literatura testimonial como literatura de mujeres. Un análisis interior de la relación entre realidad y ficción, entre Elena, Jesusa o Tinísima
Methods for the genetic manipulation of marine bacteria
No full textGenetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the biotechnological potential associated with environmental bacteria and to utilize the functional properties of specific genes when inserted into an appropriate host. In the past years, marine bacteria have received increasing attention because they represent a fascinating reservoir of genetic and functional diversity that can be utilized to fuel the bioeconomy sector. However, there is an urgent need for an in-depth investigation and improvement of the genetic manipulation tools applicable to marine strains because of the paucity of knowledge regarding this. This review aims to describe the genetic manipulation methods hitherto used in marine bacteria, thus highlighting the limiting factors of the different techniques available today to increase manipulation efficiency. In particular, we focus on methods of natural and artificial transformations (especially electroporation) and conjugation because they have been successfully applied to several marine strains. Finally, we emphasize that, to avoid failure, future work should be carried out to establish tailored methodologies for marine bacteria.How to cite: Zeaiter Z, Mapelli F, Crotti E, et al. Methods for the genetic manipulation of marine bacteria. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.03.003. Keywords: Conjugation, Electroporation, Genetic manipulation, Genome engineering, Marine bacteria, Metagenomic library, Natural competence, Next-generation DNA sequencing, Single-cell genome analysis, Suicide vectors, Transformatio
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