5,705 research outputs found
The investigation of the properties and behaviour of superficial bladder cancer by an in vitro explant tumour system, and its use in the response to conventional and novel intravesical cytotoxic agents /cTimothy John Crook.
No full textAim: To produce a four-dimensional model of superficial transitional cell carcinoma of the bladder in vitro for the assessment of therapeutic agents used as intravesical therapy, and which is consistent and amenable to rationalised experiments. Introduction: Superficial transitional cell carcinoma of the bladder is more common compared to the invasive type, but has a high probability of recurrence. The various modalities of control include cytoscopic resection or diathermy ablation, as well as intravesical chemotherapeutic adjuncts such as the anthracyclines, Mitomycin C and immunotherapy with BCG. The laboratory investigation of these intravesical agents is by assays on monolayers of tumour cell lines. Animal models are able to give more accurate representations of clinical tumours in certain cancer types. However, there is no reliable animal model of superficial transitional cell carcinoma because of the difficulty in keeping the tumour both non-invasive and consistent in size and tumour grade. Methods: The MGH-U1 cell line was transfected with green fluorescent protein in its parental and resistant forms. Rat bladder explant cultures were established, and the explants seeded with the transfected cell lines. Visualisation of the resulting colonies was by fluorescent confocal microscopy. Fluorescence intensity and area of the colonies were recorded for each time point. Cytotoxic drugs were applied to the tumour colonies for 1 hour and the effects observed over several days. Results: The MGH-U1 cell line stably expressed Green Fluorescent Protein, and maintained its characteristics. Tumour colonies were successfully established on the explants, and visualised for up to 11 days after seeding. Mean area of the colony was used as a measure of colony growth. Experiments with cytotoxic agents showed that high concentrations of drugs similar to those in use clinically could be applied to the model, with continuing growth of the colonies.</p
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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The improvisation of Hal Crook
No full textThe purposes of this essay are to (1) transcribe and analyze four improvised solos by contemporary jazz trombonist Hal Crook, (2) provide numerous examples of melodic, harmonic, rhythmic and stylistic aspects of his playing, (3) categorize Crook as a major figure in contemporary jazz trombone, and (4) interview Mr. Crook about the state of jazz trombone, his improvisational philosophy, and the value of jazz in higher education. The paper highlights the improvisational style of Hal Crook on a variety of standard songs written before 1950 and includes the solo transcriptions on the following: "Because of You," "Darn That Dream," "My Funny Valentine," and "Suddenly It's Spring."</p
BBC Radio Censorship Rows: Lessons From History
No full textTim Crook examines three major crises to have shaken the Corporation and concludes: ‘Mistakes are not permitted, rarely forgiven, and the future is less certain and secure for the BBC than it has ever been in its 92-year history
The Secret Lives Of A Secret Agent: The Mysterious Life and Times of Alexander Wilson
No full textAlexander Wilson was a major spy fiction writer of the 1920s and 1930s but disappeared and published nothing more after 1940. His 9-year-old son was told he had been killed at El Alamein in 1942. More than 60 years later that young boy, Mike Shannon, asks Goldsmiths academic Tim Crook to find out more.
This unique investigation into the life of a secret agent and writer of espionage reveals a significant author ‘lost to history’ and whose intelligence legend masked a double life more dramatic, complex, romantic and tragic than any character or plot conjured by the world of spy fiction.
The book analyses the rituals of espionage, the ethics of intelligence work, and its relationship with popular spy fiction in the 20th century. It assesses the cultural capital of fictional and journalistic representations of intelligence operations, and constitutes an original body of research in studying a significant and important 'literary spy' overlooked and hitherto not recognised by previous and existing academic criticism of the subjec
sj-pdf-1-jhs-10.1177_17531934221098504 - Supplemental material for Exploration of rehabilitation through the use of virtual reality interventions for patients with hand conditions: a scoping review
No full textSupplemental material, sj-pdf-1-jhs-10.1177_17531934221098504 for Exploration of rehabilitation through the use of virtual reality interventions for patients with hand conditions: a scoping review by Stefanie Frances Gibb (née Andrew), Carol Julie Clark, Zoë Alexandra Sheppard and Timothy Bernard Crook in Journal of Hand Surgery (European Volume)</p
Money piece by Timothy P. Agnew, chief executive officer of the Finance Author
No full textMoney piece by Timothy P. Agnew, chief executive officer of the Finance Authority of Maine, about the increased availability of credit for Maine\u27s small businesses
DUSP16 is an epigenetically regulated determinant of JNK signalling in Burkitt's lymphoma
No full textBACKGROUND: The mitogen-activated protein kinase (MAPK) phosphatases or dual specificity phosphatases (DUSPs) are a family of proteins that catalyse the inactivation of MAPK in eukaryotic cells. Little is known of the expression, regulation or function of the DUSPs in human neoplasia. METHODS: We used RT-PCR and quantitative PCR (qPCR) to examine the expression of DUSP16 mRNA. The methylation in the DUSP16 CpG island was analysed using bisulphite sequencing and methylation-specific PCR. The activation of MAPK was determined using western blotting with phospho-specific antibodies for extra-cellular signal-related kinase (ERK), p38 and c-Jun N-terminal kinase (JNK). The proliferation of cell lines was assessed using the CellTiter 96 Aqueous One assay. RESULTS: The expression of DUSP16, which inactivates MAPK, is subject to methylation-dependent transcriptional silencing in Burkitt’s Lymphoma (BL) cell lines and in primary BL. The silencing is associated with aberrant methylation in the CpG island in the 50 regulatory sequences of the gene blocking its constitutive expression. In contrast to BL, the CpG island of DUSP16 is unmethylated in other non-Hodgkin’s lymphomas (NHLs) and epithelial malignancies. In BL cell lines, neither constitutive nor inducible ERK or p38 activity varied significantly with DUSP16 status. However, activation of JNK was increased in lines with DUSP16 methylation. Furthermore, methylation in the DUSP16 CpG island blocked transcriptional induction of DUSP16, thereby abrogating a normal physiological negative feedback loop that limits JNK activity, and conferred increased cellular sensitivity to agents, such as sorbitol and anthracycline chemotherapeutic agents that activate JNK. CONCLUSION: DUSP16 is a new epigenetically regulated determinant of JNK activation in BL. British Journal of Cancer (2010) 103, 265-274. doi: 10.1038/sj.bjc.6605711 www.bjcancer.com Published online 15 June 2010 (C) 2010 Cancer Research U
Timothy Meyer serves as a contributing author for UN report
No full textAssistant Professor Timothy Meyer served as a contributing author for the United Nations Industrial Development Organization\u27s report titled Networks for Prosperity: Connecting Development Knowledge Beyond 2015. The document, which was released during November, analyzes the nexus between the global connectedness of a country and its economic success, sustainability and government effectiveness. Meyer was one of only approximately 20 academic and practical experts from around the world selected to serve as a contributor after a global call for proposals.
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Selected Contributions of Sister Mary Berenice Beck, O.S.F. to Nursing in the United States, 1923-1956
No full textby Sister M. Timothy Costello.Typescript.Thesis (M.S.N.)--Catholic University of America.Bibliography: leaves 44-47.Also available in microfilm
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