1,720,962 research outputs found
Identification of bifidobacteria from fermented milk products
No full textSix samples of fermented milk preparations were examined for the presence of bifidobacteria. Identification was based on fermentation tests, genetic relatedness studies and electrophoretic analysis. Contrary to label information, Bifidobacterium animalis was the only species present
Bifidobacterium saeculare: a New Species Isolated from Feces of Rabbit
No full textIn a previous investigation, some bifidobacteria isolated from rabbit feces were characterized as “unassigned homology group I”. New evidence derived from studies on DNA-DNA homology, phenotypic characters and electrophoretic patterns of proteins and of isozymes suggests that “unassigned homology group I” represents a new species of the genus Bifidobacterium, described in this paper as Bifidobacterium saeculare (type strain ATCC 49392). © 1991, Gustav Fischer Verlag, Stuttgart · New York. All rights reserved
Phase variations in Bifidobacterium animalis
No full textStrains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as Bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. The rate of transition in a switching system from opaque to transparent colonies and vice versa was determined. Differences in protein components and in penicillin-binding proteins (PBPs) of the cells from different colony types are shown. © 1992 Springer-Verlag New York Inc
Different electrophoretic patterns of cellular soluble proteins in Bifidobacterium animalis
No full textTwenty-nine strains of Bifidobacterium animalis, a species found only in animal habitats, were studied. Strains from known origins such as rat, rabbit and chicken feces and strains isolated from extrabody environments such as sewage and fermented milk products were examined. The intestinal origins of strains isolated from sewage and fermented milk products were determined by means of the comparison of electrophoretograms of cellular soluble proteins. Unknown origins of strains were recognized as being rabbit or chicken intestinal tract
Characterization of the plasmid pVS809 from Bifidobacterium globosum
No full textA plasmid from a B. globosum strain was cut with 38 restriction enzymes and a physical map was constructed. Out of a total of 121 clones from curing experiments, plasmid was lost in 58% and 100% for acridine orange and ethidium bromide curing agent respectively. The plasmid does not exist as a chromosomal integrated form. An attempt to determine phenotypic characters encoded by the plasmid was made by electrophoretic analyses of the total proteins
Effect of growth temperature on the biosynthesis of cell wall proteins from Bifidobacterium globosum
No full textSeventy strains of Bifidobacterium globosum isolated from gastrointestinal tracts of different animals were studied. Strains were grown at temperatures ranging from 25 to 46.5°C in order to examine changes both in the expression of bifidobacterial outer proteins (BIFOPs) and in their hydrophobic properties. It was observed that the expression of BIFOPs found on the cell-surface changes according to growth temperature, with quantitative and/or qualitative variations. Generally speaking, it was observed that BIFOP expression at low-growth temperature was considerably attenuated, while at medium- and high-growth temperature it increased. Furthermore, at high-growth temperatures, the presence of a new common protein was detected in all the strains studied. Cells from B. globosum strains grown under different temperature conditions were studied in terms of their cellular hydrophobicity properties. At medium-growth temperature, the cell hydrophobicity was strictly correlated with BIFOP expression, while at low and high-growth temperatures, the presence of BIFOP only partially influenced the hydrophobic features
Bifidobacterial cell wall proteins (BIFOP) in Bifidobacterium globosum
No full textNearly 150 strains of Bifidobacterium globosum were isolated from faeces of calf, chicken, lamb, rabbit and rat, from sewage, from rumen content and from human infant faeces between 1962 and 1973 and scored by SDS-PAGE for the presence of cell-wall-related proteins, i.e. BIFOP (bifid outer proteins); their apparent molecular masses ranged from 94.5 to 34 kDa and were designated A to L. Purified preparations from six of these ten proteins were employed to produce polyclonal rabbit antisera for use in immunoblots to investigate the interrelationships of the major antigens, A, B and C (94.5-85.5 kDa) and their distribution in strains of various origin. Two antigens differently migrating (or polymorphic forms) reacted with anti-BIFOP F serum (called F- and F+); the identity of BIFOP E with respect to these antigens was studied with anti-E serum. Only one antigen in all strain preparations reacted to anti-BIFOP H serum, which was raised against an antigen purified from a 13.5-MDa plasmid-bearing strain from rumen. © 1993
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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