1,721,036 research outputs found
Fetal adnexa derived stem cells from domestic animal: progress and perspectives.
No full textThe fetal adnexa such as umbilical cord, amnion and amniotic fluid have been proposed as ideal sources of different stem cell lineages. Use of adnexal tissue has many potential advantages, including the noninvasive nature of the isolation procedure, the large tissue mass from which cells can be harvested with high efficiency and the potential of these cells to differentiate. Moreover, particularly in human medicine, the harvesting of these tissues is more ethically acceptable making these sources of stem cells very attractive for regenerative therapies and biotechnological applications. The adnexal tissue cells preserve some of the characteristics of the primitive embryonic layers from which they originate. Indeed, many studies indicate that these stem cells exhibit some features of embryonic stem cells as expression of embryonic markers and proliferation capability, without showing immunogenicity. However, the differentiation potential of these cells, either in vivo or in vitro, is intermediate between the pluripotent embryonic stem cells and the multipotent adult stem cells. Non-embryonic extra-fetal derived stem cells have opened new perspectives for developmental biology and for regenerative medicine, not only in humans but also in animals. In this update, we report the state of the art of fetal adnexa-derived stem cells from domestic animals and analyze their applications and potential uses in veterinary medicine
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Characterization of equine amnion-derived Mesenchymal Stem Cells (MSCs) after cryopreservation
No full textDerivation of Presumptive Stem Cells from the Bovine Amnion
No full textAmnion is a noncontroversial, inexhaustible source that can be harvested noninvasively at low cost; moreover, presumptive stem cells are not acutely rejected when transplanted. As in human medicine, stem cells isolated from animal term placenta are promising candidates for disease treatment, specifically for their plasticity and their reduced immunogenicity and high anti-inflammatory potential. The aim of this work was to obtain, for the first time, presumptive stem cells from the epithelial (AECs) and mesenchymal (AMCs) portions of the bovine amniotic membrane to be used for clinical applications, in particular for the treatment of mastitis. AECs and AMCs were isolated following the method used by Lange Consiglio et al. (2010) in horse. Our preliminary results show that cells from epithelial portion displayed a polygonal morphology whereas those from mesenchymal site were fibroblastic-like. Both cell lines were able to adhere to the plastic dishes and resulted positive for MSC-markers (CD44, CD29, CD105, CD73 and CD166) and negative for the hematopoietic marker (CD34) from passage 1 (P1) to P3. Obtained cells showed a pluripotent profile by expressing c-Myc, Oct-4 but not Nanog at every passage studied. AECs and AMCs MHC-I at every passage whereas only AMCs did not express MHC-II at the first passage. Further studies, including pre-clinical and a deeper evaluation of immunological properties, need to be performed for the in vivo applications in order to better understand the role of these cells in cellular therapy
Tenogenic differentiation of equine mesenchymal progenitor cells under indirect co-culture.
No full textPurpose: Adult bone marrow mesenchymal stem cells (BM-MSCs) are a potential cell source for tendon repair in direct cell therapy and tissue engineering investigations. The purpose of this study was to evaluate the tenogenic induction of undifferentiated BM-MSCs under indirect co-culture technique with trimmed native tendon tissue. Since the horse represents a preferred species to study tendon regenerative strategies, this work was conducted on equine BM-MSCs. Methods: Equine BM-MSCs were co-cultured in a transwell system with tendon tissue fragments. The BM-MSC tenogenic differentiation was evaluated by cytochemical staining and real time PCR for gene expression. Cell viability in tendon fragments and cultured cells was analyzed. Results: Our results indicate that under indirect co-culture with native and healthy tendon tissue the BM-MSCs expressed tendon-specific markers such as decorin, tenomodulin, tenascin-C, and collagen type I. They also retained a tenocyte-like phenotype during monolayer culture. Conclusions: Data are very encouraging for future in vitro investigations into committing cells to the tenogenic lineage without adding growth factors or serum to the culture medium for both cell therapy and tissue engineering
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
The epithelial-mesenchymal transition (EMT) in equine amniotic multipotent progenitor cells induces changes of the cell glycan profile.
No full textTechnique for repeated collection of blood that has just perfused the pineal gland of sheep.
No full textA long catheter was inserted into the superior sagittal sinus of sheep and was maneuvered to the level of the rectus sinus. The catheter was then secured to the head, neck, and shoulder of the animal. The distal end of the catheter was connected to a 3-way valve through which blood samples were collected and heparin was injected. Blood loss during surgery was minimal; recovery was quick and complete. The simple surgical technique enabled the collection of blood that has just perfused the pineal gland
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