1,720,954 research outputs found
Harnessing label-free, second harmonic generation microscopy for research and diagnosis of osteosarcoma
No full textOsteosarcoma (OS), the most common primary cancer of bone, predominantly affects paediatric patients and is associated with poor prognoses. OS is characterised by the production of an immature bone matrix, resulting in aberrant extracellular matrix (ECM) components, including collagen. In recent years, label-free optical techniques have emerged as promising tools for non-invasive tumour identification, by detecting structural alterations in the ECM. This thesis explores the potential of label-free, second harmonic generation (SHG) microscopy as a tool for research and diagnosis of OS, aiming to enhance our understanding of this malignancy and provide valuable insights for OS diagnosis and treatment. The central hypothesis of this study is that label-free SHG microscopy can identify disease-specific matrix signatures in OS.In the first results chapter, initial optimisation of SHG imaging and analysis protocols are described, enabling extraction of collagen fibre parameters from healthy and malignant bone biopsies. The optimised protocol highlights the importance of imaging parameters, including field of view size, for accurate quantification of collagen fibre parameters within human bone biopsies. Quantified collagen parameters included: fibre length, width, straightness, and orientation. The second results chapter presents the diagnostic capability of SHG microscopy and quantitative analyses of collagen in distinguishing OS biopsies from healthy bone and other skeletal malignancies. Here, SHG microscopy revealed a significant reduction in collagen fibre lengths in OS compared to healthy bone (average fibre length – bone: 27.88 µm 2.18, OS: 23.77 µm 1.00, p < 0.001) – a phenomenon which was exacerbated in late-stage OS biopsies. These findings provide increased awareness of the role of collagen alterations in OS stage and disease progression, highlighting the diagnostic and prognostic potential of aberrant collagen identified via SHG imaging. In the third results chapter, combined use of SHG microscopy and an established preclinical model of OS – c-fos transgenic mice – enabled temporal characterisation of OS-associated collagen phenotypes, enquiring whether collagen aberrations in this mouse model reflect human manifestations of the disease. This research set out to advance our understanding of OS pathology and introduce the examination of aberrant collagen using label-free, SHG microscopy as a powerful diagnostic tool. It underscores the critical role of precise imaging parameters and offers a bridge between animal models and human disease, laying the foundation for possible innovative advancements in OS research and patient care
Dataset in support of the thesis 'Harnessing label-free, non-linear microscopy for research and diagnosis of osteosarcoma'
No full textData associated with my doctoral thesis. More specifically, collagen fibre parameters extracted from second harmonic generation images of clinical bone and osteosarcoma biopsies. </span
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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