891 research outputs found
MIRACLE at ImageCLEFanot 2007: Machine Learning Experiments on Medical Image Annotation
No full textThis paper describes the participation of MIRACLE research consortium at the ImageCLEF Medical Image Annotation task of ImageCLEF 2007. Our areas of expertise do not include image analysis, thus we approach this task as a machine-learning problem, regardless of the domain. FIRE is used as a black-box algorithm to extract different groups of image features that are later used for training different classifiers based on kNN algorithm in order to predict the IRMA code. The main idea behind the definition of our experiments is to evaluate whether an axis-by-axis prediction is better than a prediction by pairs of axes or the complete code, or vice versa
MIRACLE at ImageCLEFannot 2008: Classification of Image Features for Medical Image Annotation
No full textThis paper describes the participation of MIRACLE research consortium at the ImageCLEF Medical Image Annotation task of ImageCLEF 2008. A lot of effort was invested this year to develop our own image analysis system, based on MATLAB, to be used in our experiments. This system extracts a variety of global and local features including histogram, image statistics, Gabor features, fractal dimension, DCT and DWT coefficients, Tamura features and coocurrency matrix statistics. Then a k-Nearest Neighbour algorithm analyzes the extracted image feature vectors to determine the IRMA code associated to a given image. The focus of our experiments is mainly to test and evaluate this system in-depth and to make a comparison among diverse configuration parameters such as number of images for the relevance feedback to use in the classification module
Structure of paused transcription complex Pol II–DSIF–NELF.
No full textMetazoan gene regulation often involves the pausing of RNA polymerase II (Pol II) in the promoter-proximal region. Paused Pol II is stabilized by the protein complexes DRB sensitivity-inducing factor (DSIF) and negative elongation factor (NELF). Here we report the cryo-electron microscopy structure of a paused transcription elongation complex containing Sus scrofa Pol II and Homo sapiens DSIF and NELF at 3.2 Å resolution. The structure reveals a tilted DNA–RNA hybrid that impairs binding of the nucleoside triphosphate substrate. NELF binds the polymerase funnel, bridges two mobile polymerase modules, and contacts the trigger loop, thereby restraining Pol II mobility that is required for pause release. NELF prevents binding of the anti-pausing transcription elongation factor IIS (TFIIS). Additionally, NELF possesses two flexible ‘tentacles’ that can contact DSIF and exiting RNA. These results define the paused state of Pol II and provide the molecular basis for understanding the function of NELF during promoter-proximal gene regulation
Fehler und Sicherheitskultur in der stationären Pflege
No full textCramer H. Fehler und Sicherheitskultur in der stationären Pflege. Bielefeld: Universität Bielefeld; 2014
An experimentally generated peptide database increases the sensitivity of XL-MS with complex samples
No full textCross-linking mass spectrometry (XL-MS) is steadily expanding its range of applications from purified protein complexes to more complex samples like organelles and even entire cells. One main challenge using non-cleavable cross-linkers is the so-called n2 problem: With linearly increasing database size, the search space for the identification of two covalently linked peptides per spectrum increases quadratically. Here, we report an alternative search strategy that focuses on only those peptides, which were demonstrated to cross-link under the applied experimental conditions. The performance of a parallel XL-MS experiment using a thiol-cleavable cross-linker enabled the identification of peptides that carried a cleaved cross-link moiety after reduction and hence were involved in cross-linking reactions. Based on these identifications, a peptide database was generated and used for the database search of the actual cross-linking experiment with a non-cleavable cross-linker. This peptide-focused approach was tested on protein complexes with a reported structural model and obtained results corresponded well to a conventional database search. An application of the strategy on in vivo cross-linked Bacillus subtilis and Bacillus cereus cells revealed a five- to tenfold reduction in search time and led to significantly more identifications with the latter species than a search against the entire proteome
Structures of transcription preinitiation complex engaged with the +1 nucleosome
No full textAbstract
The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how the PIC assembles within chromatin. In the yeast
Saccharomyces cerevisiae
, PIC assembly occurs adjacent to the +1 nucleosome that is located downstream of the core promoter. Here we present cryo-EM structures of the yeast PIC bound to promoter DNA and the +1 nucleosome located at three different positions. The general transcription factor TFIIH engages with the incoming downstream nucleosome and its translocase subunit Ssl2 (XPB in human TFIIH) drives the rotation of the +1 nucleosome leading to partial detachment of nucleosomal DNA and intimate interactions between TFIIH and the nucleosome. The structures provide insights into how transcription initiation can be influenced by the +1 nucleosome and may explain why the transcription start site is often located roughly 60 base pairs upstream of the dyad of the +1 nucleosome in yeast
Pflegerische Unterstützung kranker Kinder. Eine Analyse von Einschätzungsinstrumenten.
No full textCramer H, Wingenfeld K. Pflegerische Unterstützung kranker Kinder. Eine Analyse von Einschätzungsinstrumenten. Pflege & Gesellschaft. 2016;21(3):261-274
Ein neues Instrument zur Erfassung des pflegerischen Unterstützungsbedarfs von kranken Kindern und ihren Familien
No full textWingenfeld K, Cramer H. Ein neues Instrument zur Erfassung des pflegerischen Unterstützungsbedarfs von kranken Kindern und ihren Familien. Kinderkrankenschwester. Fachzeitschrift der Gesundheits- und Kinderkrankenpflege. 2014;33(12):474-477
Die Einschätzung des pflegerischen Unterstützungsbedarfs kranker Kinder und ihrer Eltern
No full textWingenfeld K, Cramer H. Die Einschätzung des pflegerischen Unterstützungsbedarfs kranker Kinder und ihrer Eltern. Veröffentlichungsreihe des Instituts für Pflegewissenschaft an der Universität Bielefeld. Vol P14-151. Bielefeld: Institut für Pflegewissenschaft an der Universität Bielefeld; 2014
Erprobung eines neuen Einschätzungsinstruments für die Pflege kranker Kinder und Jugendliche.
No full textWingenfeld K, Cramer H. Erprobung eines neuen Einschätzungsinstruments für die Pflege kranker Kinder und Jugendliche. Veröffentlichungsreihe des Instituts für Pflegewissenschaft an der Universität Bielefeld. Vol P16-153. Bielefeld: Institut für Pflegewissenschaft an der Universität Bielefeld; 2016
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