196,862 research outputs found
Letter from M. E. Wright, Prairie Mer Rouge, Louisiana, to Sally Cotten, September 1, 1884
No full textLetter from M. E. Wright, Prairie Mer Rouge, Louisiana, to Alice Cotten Houston, April 25, 1880
No full text
Letter from M. E. Wright, Prairie Mer Rouge, Louisiana, to Alice Cotten Houston, February 21, 1886
No full textLetter from M. E. Wright, Prairie Mer Rouge, Louisiana, to Alice Cotten Houston, December 5, 1880
No full textLipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus
No full textEndotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells) in the presence of entry-competent adenovirus particles. Toxicity can be observed with as little as 100 ng/ml free LPS or 100 pg/ml LPS when the LPS is assembled into polylysine/adenovirus complexes. Simple and effective methods of removing the contaminating LPS using either a polymyxin B resin or Triton X-114 extraction are described. Treatment of DNA samples to remove LPS eliminates the toxicity to primary cells
Letter from M. E. Wright, Prairie Mer Rouge, Louisiana, to Alice Cotten Houston, March 1, 1885
No full textLetter from M. E. Wright, Prairie Mer Rouge, Louisiana, to Mercer Cotten, February 6, 1881
No full textLetter from M. E. Wright, Prairie Mer Rouge, Louisiana, to Mercer and Sally Cotten, January 16, 1881
No full textLetter from W. M. Green, Columbus, Mississippi, to Mary Wright, February 1, 1864
No full textW. M. Green also forwarded to his sister a letter from Robert Cotten, Washington, Arkansas, to Bishop Green, December 23, 1863
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