1,721,454 research outputs found
Evaluation of gene editing strategies based on CRISPR/Cas9 to study specific alleles of LGALS3 associated with the risk of papillary thyroid carcinoma
No full textGalectin-3 is involved in thyroid carcinogenesis. The single nucleotide polymorphism
(SNP) rs4644 (c.191C>A, p.Pro64His) within its encoding gene, LGALS3, is
completely conserved across phylogenetically distant organisms, and it was predicted
to be damaging by analysis performed with Polyphen and SIFT. Thus, we
hypothesized that this SNP could affect the risk to develop DTC. The hypothesis was
corroborated by a case-control association study (1155 DTC cases and 1222 controls),
showing a protective role for the carriers of the Histidine-64 (OR=0.66; IC 95% 0.46-
0.93) versus the Proline-64 allele.
To better understand the role of this SNP, cell lines underwent gene editing for
altering the specific LGALS3 genotype.
The “generation” of the cell lines was performed with a Clustered Regularly
Interspaced Short Palindromic Repeats-associated Endonuclease 9 (CRISPR/Cas9)
system. Different vectors and approaches for DNA strand breaks induction, as well as
alternative strategies of delivery, were employed and compared to each others in order
to identify the most effective in terms of specificity, time, and costs.
Isogenic cell lines were obtained following gene editing although with variable
results, depending from the strategy employed.
Further steps will include the identification of possible off targets and the evaluation
of how global gene expression is affected by Pro to His variation. In the future these
cells will be helpful to understand the biologic role of this aminoacid change within
galectin-3
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Of Earthquakes and Identities: Naming Southern Italy in the Works of Sir William Hamilton and Patrick Brydone
No full textThe essay explores the multiple contaminations between scientific and literary languages, textual typologies and linguistic registers in the writings of Sir William Hamilton and Patrick Brydone
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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