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Novel Approaches for the Application of Mitochondrial Respirometry in Human Studies
No full textThough mitochondrial research has become more prominent in the last forty years, most methodologies to study mitochondria have been focused on cell and animal models. Respirometry, the assessment of mitochondrial function measured as oxygen consumption by Complex IV of the electron transport chain, is the “gold standard” for mitochondrial assessment, yet it is rarely utilized in human samples. This is due to two fundamental methodological challenges: (1) samples for respirometry must be processed and ran within hours of acquisition on specialized and complex equipment, and (2) human sampling for respirometry must be acquired using invasive tissue biopsies. While we as a field realize the importance of oxygen consumption as a measurement of mitochondrial function, the current methodologies are now limiting the types of biological and clinical questions we can ask and answer. Addressing the first challenge preventing widespread adoption of respirometry, the recently developed RIFS (Respirometry In previously-Frozen Specimen) assay allows for the measurement of maximal respiratory capacity from cells or tissues which have been previously frozen. This allows samples to be processed and then stored or biobanked for later respirometry assessment, ultimately making respirometry methodologies significantly easier, cheaper, and more accessible. To the second challenge though, there has been minimal breakthrough.In this thesis we will cover novel methodologies overcoming challenges which are paramount to the expansion of respirometry methodologies for clinical applications. We will discuss the RIFS protocol in detail, describe the methodologies developed to perform a first-of-its-kind high-throughput and high-resolution respirometry study of the human brain, and will detail the development of a technique to measure mitochondrial respiration in cells human salivary leukocytes. Together, these chapters present novel methodologies designed to overcome the primary limitations of performing respirometry in human studies
Mitochondrial Heterogeneity in Metabolic Diseases
No full textMitochondria have distinct architectural features and biochemical functions consistent with cell-specific bioenergetic needs. However, as imaging and isolation techniques advance, heterogeneity amongst mitochondria has been observed to occur within the same cell. Moreover, mitochondrial heterogeneity is associated with functional differences in metabolic signaling, fuel utilization, and triglyceride synthesis. These phenotypic associations suggest that mitochondrial subpopulations and heterogeneity influence the risk of metabolic diseases. This review examines the current literature regarding mitochondrial heterogeneity in the pancreatic beta-cell and renal proximal tubules as they exist in the pathological and physiological states; specifically, pathological states of glucolipotoxicity, progression of type 2 diabetes, and kidney diseases. Emphasis will be placed on the benefits of balancing mitochondrial heterogeneity and how the disruption of balancing heterogeneity leads to impaired tissue function and disease onset
O conceito de revelação na Teologia Latino-Americana: uma abordagem a partir de João Batista Libanio
No full textThis master research has as a goal to analyse the concept of revelation on the Latin- Americam theology, from the approach of João Batista Libanio theologian. It will verify how Libanio systematized the notion of revelation in the Teology of the release and which implications of this systematization for the Christian praxis and for the theology itself. For that, it approaches in the first chapter, the notion of revelation in the conciliar magisterium since the Council of Trent to the Council of Vatican II, reaching the corresponding translation of this concept in Latin-American Theology, specially through the approach done by Libanio. The second chapter tries to understand how Libanio creates the concept of revelation within the logical of historical liberation and which categories the author uses to see the divine action in the human history. The third chapter, according to Libanio’s theology, thematizes the revelation in the view of the option for the poor people, from the disclosure of the poverty as consequence of the sin and from the Basics Ecclesial Communities. ) CEBs. Finally, in the last chapter, tries to show the God’s image that emerge from one view of revelation from the context of preferential option for the poor people and by its release and as Libanio, starting from God’s idea who reveals himself in the release of the poor people, he tries to elaborate one new Fundamental Theology.A presente pesquisa de mestrado tem como objetivo analisar o conceito de revelação na teologia latino-americana, a partir da abordagem do teólogo João Batista Libanio. Trata-se de verificar como Libanio sistematizou a noção de revelação dentro da Teologia da Libertação e quais as implicações dessa sistematização para a práxis cristã e para a própria teologia. Para isso, aborda-se no primeiro capítulo, a noção de revelação no magistério conciliar a partir do Concílio de Trento até o Concílio Vaticano II, chegando à consequente tradução desse conceito na teologia latino-americana, especialmente por meio da abordagem realizada por Libanio. No segundo capítulo busca-se entender como Libanio elabora o conceito de revelação dentro da lógica da libertação histórica e quais categorias o autor usa para sondar o agir divino na história humana. O terceiro capítulo, seguindo a teologia de Libanio, tematiza a revelação na perspectiva da opção pelos pobres, da denúncia da pobreza como consequência do pecado e a partir das Comunidades Eclesiais de Base (CEBs). Por fim, no último capítulo, busca-se esboçar a imagem de Deus que emerge de uma visão da revelação a partir do contexto de opção preferencial pelos pobres e pela sua libertação e como Libanio, partindo da ideia do Deus que se revela na libertação dos pobres, procura elaborar uma nova Teologia Fundamental
O conceito de revela??o na Teologia Latino-Americana : uma abordagem a partir de Jo?o Batista Libanio
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Previous issue date: 2016-03-17This master research has as a goal to analyse the concept of revelation on the Latin-
Americam theology, from the approach of Jo?o Batista Libanio theologian. It will verify how
Libanio systematized the notion of revelation in the Teology of the release and which
implications of this systematization for the Christian praxis and for the theology itself. For
that, it approaches in the first chapter, the notion of revelation in the conciliar magisterium
since the Council of Trent to the Council of Vatican II, reaching the corresponding
translation of this concept in Latin-American Theology, specially through the approach done
by Libanio. The second chapter tries to understand how Libanio creates the concept of
revelation within the logical of historical liberation and which categories the author uses to
see the divine action in the human history. The third chapter, according to Libanio?s theology, thematizes the revelation in the view of the option for the poor people,from the disclosure of the poverty as consequence of the sin and from the Basics Ecclesial Communities.) CEBs. Finally, in the last chapter, tries to show the God?s image that emerge from one view of revelation from the context of preferential option for the poor people and by its release and as Libanio, starting from God?s idea who reveals himself in the release of the poor people, he tries to elaborate one new Fundamental Theology.A presente pesquisa de mestrado tem como objetivo analisar o conceito de revela??o na teologia latino-americana, a partir da abordagem do te?logo Jo?o Batista Libanio. Trata-se de verificar como Libanio sistematizou a no??o de revela??o dentro da Teologia da Liberta??o e quais as implica??es dessa sistematiza??o para a pr?xis crist? e para a pr?pria teologia. Para isso, aborda-se no primeiro cap?tulo, a no??o de revela??o no magist?rio conciliar a partir do Conc?lio de Trento at? o Conc?lio Vaticano II, chegando ? consequente tradu??o desse conceito na teologia latino-americana, especialmente por meio da abordagem realizada por Libanio. No segundo cap?tulo busca-se entender como Libanio elabora o conceito de revela??o dentro da l?gica da liberta??o hist?rica e quais categorias o autor usa para sondar o agir divino na hist?ria humana. O terceiro cap?tulo, seguindo a teologia de Libanio, tematiza a revela??o na perspectiva da op??o pelos pobres, da den?ncia da pobreza como consequ?ncia do pecado e a partir das Comunidades Eclesiais de Base (CEBs). Por fim, no ?ltimo cap?tulo, busca-se esbo?ar a imagem de Deus que emerge de uma vis?o da revela??o a partir do contexto de op??o preferencial pelos pobres e pela sua liberta??o e como Libanio, partindo da ideia do Deus que se revela na liberta??o dos pobres, procura elaborar uma nova Teologia Fundamental
B&B Höntän kartano : sosiaalisen median markkinointisuunnitelma
No full textTämän toiminnallisen opinnäytetyön tavoitteena oli luoda ensimmäisen vuoden markkinointisuunnitelma sekä lanseerausmarkkinointisuunnitelma suunnitteilla olevalle B&B-majatalolle Siilinjärven Hamulaan. Toimeksiantajana toimii Osto- ja myyntiliike Vilin valinta ky, jonka yrittäjäpariskunta osti vuonna 2014 Hamulasta pienen kartanon. Pariskunta huomasi kartanossa olevan potentiaalia pienimuotoisen B&B-majatalon pyörittämiselle, joten he päättivät alkaa suunnitella tätä tarkemmin. Pariskunnalla ei itsellään ole varsinaista kokemusta markkinoinnista, joten he päättivät antaa sen puolen opinnäytetyön aiheeksi.
Opinnäytetyön teoriaosuudessa käsitellään markkinointia, markkinointisuunnitelman tekoa, lanseerausmarkkinointia sekä markkinointia sosiaalisessa mediassa, erityisesti Facebookissa. Opinnäytetyössä on hyödynnetty esimerkiksi SWOT-analyysia sekä markkinointi-mixiä eli 7P:n kilpailukeinomallia.
Markkinointisuunnitelman tekemistä varten tehtiin työpöytätutkimusta Siilinjärven matkailu- ja majoitustilanteesta sekä selvitettiin sähköpostikyselyllä seitsemältä eri puolilla Suomea toimivalta B&B-majatalolta heidän markkinointikeinojaan. Lisäksi haastateltiin toimeksiantajayrityksen yrittäjää Jarkko Ahokasta, jotta saatiin selville mitä he markkinointisuunnitelmalta toivovat.
Opinnäytetyöstä tulee olemaan hyötyä toimeksiantajalle pidemmällä aikavälillä, sillä he voivat hyödyntää työssä esiteltyä markkinointistrategiaa ja markkinointisuunnitelmaa myös tulevaisuudessa markkinointia suunnitellessaan. Lisäksi yrityksen on tärkeä panostaa aktiivisuuteen sosiaalisessa mediassa myös tulevaisuudessa, jotta se pystyy ylläpitämään asiakassuhteita ja asiakkaiden kiinnostusta, joten opinnäytetyössä esitellyt toiminnot sosiaalisessa mediassa auttavat tässä yritystä jatkossakin.The aim of this thesis was to create a marketing plan for the first year as well as one for the launch for a B&B guesthouse under planning in Hamula, Siilinjärvi. The client organization is Osto- ja myyntiliike Vilin valinta ky whose entrepreneurs bought a small mansion from Hamula in 2014. Soon they discovered that the mansion has huge potential for running a B&B guesthouse so they decided to start planning it more. The entrepreneurs don’t have any proper experience in marketing so they decided to give it to the author as a topic for thesis.
The theoretical part of this thesis consists of marketing, making of the marketing plan, launch marketing and marketing in social media, especially in Facebook. In this thesis the author used for example SWOT-analysis and the 7P’s Marketing Mix.
For the basis of the marketing plan the author made a desk research of the travel and accommodation situation in Siilinjärvi and made an e-mail research for seven functioning B&B guesthouses around Finland to find out about their successful marketing ways. The author also interviewed the entrepreneur Jarkko Ahokas to discover what the client organization wanted from this thesis and the marketing plan.
The client organization can benefit from this thesis in a long-term timespan because they can utilize the market-ing strategy and the marketing plan introduced in this thesis while planning marketing in the future. It is also important for the client organization to be active in social media to maintain the relationships with clients and the clients’ interest so the actions introduced in this thesis will also help them on that in the future
Yritysjärjestelmän käyttöönoton menestystekijät
No full textThis thesis examines the critical success factors of ERP project implementation.
Special focus is on procurement, sales and warehouse processes.
KCI Special Cranes was used as case company during 2001-2002.
The purpose of the project is to identify and model the key processes of 2 business units as well as parameter them in Movex ERP system.
The processes will then be implemented and results gathered during the project will be compared against the critical success factors from literature review.
The author acted as a member of the project team.
The processes were mapped using IDEFO-standard and the system was parameterized accordingly.
The results indicated that Movex ERP system suits well for project based business.
The most significant success factors were identified as understanding of strategic goals, top management dedication, project team, system supplier selection, ramp down of old IT-systems, and avoiding of modifications.
The following enhancements for the project execution were suggested: better communication, basic computer skills training, and financial compensation for project, members based on project success.Tämä diplomityö käsittelee yritysjärjestelmän käyttöönottoa ja projektin menestystekijöitä.
Tutkimus on tehty KCI Erikoisnostureiden Hyvinkään yksikössä vuosina 2001-2002.
Diplomityöntekijä oli projektiryhmän jäsen vastuualueenaan osto-, myynti- ja varastoprosessit.
Työn tarkoituksena on toteuttaa kohdeyrityksen kahdessa toimintayksikössä yritysjärjestelmän käyttöönotto osto-, myynti- ja varastoprosessien osalta ja vertailla projektin aikana kerättyjä kokemuksia järjestelmäprojektien yleisesti tunnettuihin menestystekijöihin.
Tutkimus toteutettiin osallistumalla järjestelmäprojektiin projektiryhmän jäsenenä.
Yrityksen liiketoimintaprosessit mallinnettiin IDEFO-standardin mukaisesti, järjestelmä parametroitiin, testattiin ja otettiin käyttöön.
Projektin perusteella todettiin, että Movex-yritysjärjestelmä soveltuu hyvin projektiliiketoiminnan ohjaukseen.
Projektin menestykseen vaikuttaneista tekijöistä merkittävimmät olivat strategisten tavoitteiden ymmärtäminen, johdon sitoutuminen, projektiryhmä, järjestelmän valinta, vanhojen järjestelmien alasajo, järjestelmän testaaminen ja modifikaatioiden välttäminen.
Projektin toteutukseen esitettiin seuraavia parannusehdotuksia: kommunikoinnin parantaminen, henkilökunnan tietojenkäsittelytaitojen parantaminen koulutuksilla ja projektiryhmän sitouttaminen projektiin myös rahallisesti tulospalkkauksen avulla
Kahvila Sataman Tornin alkoholitarjonnan uudistaminen
No full textOpinnäytetyö on toiminnallinen opinnäytetyö, jonka tavoitteena oli muokata Kahvila Sataman Tornin alkoholitarjontaa toiminnan kannattavuuden parantamiseksi ja luoda tuotteita, joiden avulla tuotiin lisäarvoa yrityksen alkoholimyynnille. Lisäarvoa antaviksi tuotteiksi varmistuivat yrityksen nimikkobooli ja drinkkilista.
Kahvila osallistui opinnäytetyön toteutukseen antamalla käyttöön alkoholituotteiden osto- ja myyntiluvut, joiden perusteella yksittäisten tuotenimikkeiden kannattavuutta analysoitiin. Kahvila osallistui myös lisäarvotuotteiden tuotekehitykseen.
Työssä keskityttiin tuotevalikoiman kannattavuuden analysoinnin menetelmiin. Yleisen kannattavuuslaskennan menetelmien lisäksi yksittäisten tuotenimikkeiden kannattavuutta analysoitiin ABC -analyysilla. Tekijällä on työkokemusta useista eri anniskeluravintoloista joiden tuotevalikoimien laajuus ja painopiste erosivat toisistaan merkittävästi, joten hänellä oli jo hyvä käsitys siitä, millainen alkoholituotevalikoima olisi paras case-yritykselle.
Lopputuloksena syntyi lista ehdotuksia tuotevalikoiman kannattavuuden parantamiseksi, jota yritys voi toteuttaa itsenäisesti ja joka toimii ohjenuorana tulevaisuudessa. Yritykselle suunnitellut lisäarvopalvelut toteutettiin yhteisesti opinnäytetyön tekijän sekä yrityksen toimesta.The subject of the bachelor's thesis is the renewal of the selection of alcoholic beverages to increase profitability. In addition to the renewal of the selection, products were developed in order to bring additional value to the sales of alcoholic beverages. The products that were developed during this process were the firm's signature punch and a cocktail menu.
The cafe provided purchases and sales figures for the alcoholic beverages, these figures were used in the profitability analysis of mentioned beverages. The cafe also took part in the product development phase of the products chosen to bring additional value to sales.
The theoretical section of the thesis focuses on the methods of analyzing profitability. In addition to general profitability analysis, more advanced analysis was implemented using the ABC -analysis method. The author of this thesis has worked in multiple different premises licensed to serve alcohol, many of which have wildly differing selections of alcoholic beverages. This puts the author in a good position to analyze the case firm's selection and make decisions that affect it.
The result was a list of suggestions that the case firm could implement independently and that could serve as guidelines in the future. The added-value products were developed jointly by the author and the case firm
How to Create Value in the Phonographic Industry for Consumers Between the Ages of 20-25? : A Study on whether Consumers Who Find Value in Artists Show More Behavioural Intent to Buy than Those Who Do Not, with Regard to the Phonographic Industry
No full textThe aim of this study was first to establish what factors influence the value perception of 20-25 year-olds, with regard to the phonographic industry. The second aim was to establish whether value perception has an impact on purchase disposition, with regard to the phonographic industry.
The method applied in the study was that of qualitative research. However the qualitative data was quantified to enable the comparison and presentation of the data. The data was gathered by the use of in-depth interviews which were carried out with a convenience sample of 18 respondents. The theoretical frameworks used for the interviews were the means-end chain, the multi-attribute attitude model and the model of reasoned action.
As a result of the data gathered, the research revealed attributes, consequences and final values that generate consumers’ value perception. The attributes found to create value were songs which endure the test of time, knowing the artist, the message of the songs/artist, skill and originality. The consequences of the attributes found to create value, were most notably appreciation, collectability, accompaniment, philanthropy and integrity. The attributes and consequences that led to the final values of self fulfilment, belonging, and satisfaction, were most significant factors for value perception. The study also found that perceived value influences the consumers’ buying disposition, with regard to the phonographic industry. The respondents showed more behavioural intent to purchase artists’ music in which they found value.
As a result of the findings, the author recommends marketing endeavours to focus on value creation rather than distribution and pricing.Tämä lopputyö tutki ensin mitkä tekijät vaikuttavat 20-25 vuotiaiden oletettuun arvoon
levyteollisuudessa. Sitten lopputyö tutki mikäli oletettu arvo vaikuttaa ostohalukkuuteen.
Tutkimusmenetelmänä käytettiin laadullista menetelmää, jossa vastaajia haastateltiin
henkilokohtaisesti. Tutkimuksen tuloksena löydettiin tekijöita jotka vaikuttavat olettettuun
arvoon. Tutkimus myös löysi, että olettettu arvo vaikuttaa osto halukkuuteen. Suosituksena
tutkimuksesta, musiikin markkinoinin pitäisi keskittyä arvon luomiseen
