184,804 research outputs found
Silencing disease genes in the laboratory and the clinic
No full textSynthetic nucleic acids are commonly used laboratory tools for modulating gene expression and have the potential to be widely used in the clinic. Progress towards nucleic acid drugs, however, has been slow and many challenges remain to be overcome before their full impact on patient care can be understood. Antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) are the two most widely used strategies for silencing gene expression. We first describe these two approaches and contrast their relative strengths and weaknesses for laboratory applications. We then review the choices faced during development of clinical candidates and the current state of clinical trials. Attitudes towards clinical development of nucleic acid silencing strategies have repeatedly swung from optimism to depression during the past 20 years. Our goal is to provide the information needed to design robust studies with oligonucleotides, making use of the strengths of each oligonucleotide technology
Personal Papers (MS 80-0002)
No full textCase study of a business firm that wishes to remain anonymous, referred to as "Hadley Company". This document was prepared by Associate Professor E. R. Corey for a class discussion in Ind. Mktg. 215 at Harvard Business School
Effect of chemical modifications on modulation of gene expression by duplex antigene RNAs that are complementary to non-coding transcripts at gene promoters
No full textAntigene RNAs (agRNAs) are small RNA duplexes that target non-coding transcripts rather than mRNA and specifically suppress or activate gene expression in a sequence-dependent manner. For many applications in vivo, it is likely that agRNAs will require chemical modification. We have synthesized agRNAs that contain different classes of chemical modification and have tested their ability to modulate expression of the human progesterone receptor gene. We find that both silencing and activating agRNAs can retain activity after modification. Both guide and passenger strands can be modified and functional agRNAs can contain 2?F-RNA, 2?OMe-RNA, and locked nucleic acid substitutions, or combinations of multiple modifications. The mechanism of agRNA activity appears to be maintained after chemical modification: both native and modified agRNAs modulate recruitment of RNA polymerase II, have the same effect on promoter-derived antisense transcripts, and must be double-stranded. These data demonstrate that agRNA activity is compatible with a wide range of chemical modifications and may facilitate in vivo applications. <br/
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Alien Registration- Corey, Sterling R. (Portland, Cumberland County)
No full texthttps://digitalmaine.com/alien_docs/24105/thumbnail.jp
"Closing the R&D Gap, Evaluating the Sources of R&D Spending"
No full textBoth spending and tax policies have been implemented in the United States with the goal of stimulating private sector research and development (R&D). Karier questions whether current R&D policy, especially the research and experimentation tax credit, can contribute to closing the gap between nondefense expenditures on R&D in the United States and such expenditures in other countries, such as Japan and Germany. He also explores possible changes to our current R&D policy to make it more effective.
Pengembangan panduan konseling Islami berbasis model Gerald Corey
No full textPenelitian ini dilatarbelakangi oleh ketiadaan panduan konseling Islami yang jelas dan sistematis. Tujuan penelitian adalah mengembangkan panduan konseling Islami berbasis Model Gerald Corey. Penelitian menggunakan penelitian dan pengembangan (R & D) berdasarkan model Bord & Gall dengan modifikasi oleh Sukmadinata. Tahap pengembangan meliputi: studi pendahuluan, pengembangan produk, validasi ahli dan pengguna. Teknik pengumpulan data terdiri dari kuisioner, observasi dan wawancara. Jenis data yang digunakan berupa data kuantitatif dan kualitatif. Hasil uji validasi Panduan konseling Islami berbasis Model Gerald Corey dari para ahli dan para calon pengguna menunjukkan nilai rata-rata empat (4) dengan kategori sangat tepat/sesuai. Dengan demikian, panduan koseling Islami berdasarkan model Gerald Corey telah memenuhi kriteria sangat tepat/sesuai dan layak untuk dipergunakan
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
An Integrative Approach to the Study and Application of Affect and Cognition
No full text(Statement of Responsibility) by R. Corey Remle(Thesis) Thesis (B.A.) -- New College of Florida, 1992(Electronic Access) RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE(Bibliography) Includes bibliographical references.(Source of Description) This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.(Local) Faculty Sponsor: Callahan, Charlen
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