1,721,210 research outputs found
Investigating the role of extracellular vesicles and glycosylation in cancer metastasis
Full text linkBreast and colorectal cancers present significant challenges for the UK healthcare system, placing a substantial burden on the National Health Service (NHS). Early detection is crucial for improving survival rates and reducing healthcare strain, especially as metastasis, the primary cause of mortality in these cancers, remains poorly understood. Protein glycosylation, a post-translational modification of carbohydrate structures, is a significant contributor to cancer progression and metastasis. Small extracellular vesicles (sEVs) have emerged as key players in cancer development and metastasis, facilitating intercellular communication through bioactive cargo transfer. Despite mounting evidence, the importance of sEV glycosylation in cancer and metastasis is often overlooked. Liquid biopsies, including sEV biomarker analysis, offer promising non-invasive diagnostic approaches. Given the prevalence of glycoproteins among blood cancer biomarkers, exploring the glycosylation of sEVs as biomarker targets could revolutionise early detection strategies for breast and colorectal cancer.
Efforts initially focused on optimising single-vesicle flow cytometry for sEV characterisation to ensure maximum signal and sample integrity. Subsequent analysis of breast and colorectal epithelial cells and their derived sEVs revealed pronounced HPA lectin binding in the metastatic phenotypes. A lectin microarray identified increased lectin binding of LCA and TL in ‘CD81-positive’ sEVs derived from breast cancer-associated cell lines in comparison to normal cells. Validation through single-vesicle flow cytometry confirmed these observations, prompting investigations into the diagnostic application of these lectins in distinguishing plasma-enriched sEVs from breast cancer patients and ‘healthy’ individuals. However, lectins alone did not show diagnostic significance, in contrast to the diagnostic capability of the well-established breast cancer marker EpCAM.
Overall, these results underscore the importance of optimising single-vesicle flow cytometry for comprehensive characterisation of sEVs. They also highlight the HPA lectin binding patterns of breast and colorectal metastatic cell phenotypes, which mirror the observations of their derived sEVs but with nuanced specificity attributed to tetraspanin composition. Additionally, other glycosylated targets, as recognised by TL and LCA, show increased abundance in breast cancer cell-derived sEVs. Moreover, the diagnostic capability of these lectins and EpCAM in distinguishing plasma-enriched sEVs derived from breast cancer patients from those derived from ‘healthy’ individuals is demonstrated
INVESTIGATING THE ROLE OF EXTRACELLULAR VESICLES AND GLYCOSYLATION IN CANCER METASTASIS
Full text linkBreast and colorectal cancers present significant challenges for the UK healthcare system, placing a substantial burden on the National Health Service (NHS). Early detection is crucial for improving survival rates and reducing healthcare strain, especially as metastasis, the primary cause of mortality in these cancers, remains poorly understood. Protein glycosylation, a post-translational modification of carbohydrate structures, is a significant contributor to cancer progression and metastasis. Small extracellular vesicles (sEVs) have emerged as key players in cancer development and metastasis, facilitating intercellular communication through bioactive cargo transfer. Despite mounting evidence, the importance of sEV glycosylation in cancer and metastasis is often overlooked. Liquid biopsies, including sEV biomarker analysis, offer promising non-invasive diagnostic approaches. Given the prevalence of glycoproteins among blood cancer biomarkers, exploring the glycosylation of sEVs as biomarker targets could revolutionise early detection strategies for breast and colorectal cancer.
Efforts initially focused on optimising single-vesicle flow cytometry for sEV characterisation to ensure maximum signal and sample integrity. Subsequent analysis of breast and colorectal epithelial cells and their derived sEVs revealed pronounced HPA lectin binding in the metastatic phenotypes. A lectin microarray identified increased lectin binding of LCA and TL in ‘CD81-positive’ sEVs derived from breast cancer-associated cell lines in comparison to normal cells. Validation through single-vesicle flow cytometry confirmed these observations, prompting investigations into the diagnostic application of these lectins in distinguishing plasma-enriched sEVs from breast cancer patients and ‘healthy’ individuals. However, lectins alone did not show diagnostic significance, in contrast to the diagnostic capability of the well-established breast cancer marker EpCAM.
Overall, these results underscore the importance of optimising single-vesicle flow cytometry for comprehensive characterisation of sEVs. They also highlight the HPA lectin binding patterns of breast and colorectal metastatic cell phenotypes, which mirror the observations of their derived sEVs but with nuanced specificity attributed to tetraspanin composition. Additionally, other glycosylated targets, as recognised by TL and LCA, show increased abundance in breast cancer cell-derived sEVs. Moreover, the diagnostic capability of these lectins and EpCAM in distinguishing plasma-enriched sEVs derived from breast cancer patients from those derived from ‘healthy’ individuals is demonstrated
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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