8,776 research outputs found
Targeted metabolomics data
No full textThe chemicals, standards and extraction protocol used as the same as given in Loupit et al. (2022) The analysis protocol was the same as described by Loupit et al. (2020) with some modifications and a different column (Agilent ZORBAX RRHD SB-C18 (2.1 mm x 100 mm, 1.8 μm)).
Loupit G, Prigent S, Franc C, De Revel G, Richard T, Cookson SJ, Fonayet JV. 2020. Polyphenol Profiles of Just Pruned Grapevine Canes from Wild Vitis Accessions and Vitis vinifera Cultivars. Journal of Agricultural and Food Chemistry 68, 13397-13407.
Loupit G, Prigent S, Prodhomme D, Spilmont AS, Hilbert G, Franc C, de Revel G, Ollat N, Valls Fonayet J, Cookson SJ. 2022. Identifying early metabolite markers of successful graft union formation in grapevine Horticultural Research 9, uhab070
Nitrate as a signal in Arabidopsis
No full textThis thesis describes an investigation of nitrate signalling in Arabidopsis thaliana leaf cells. The expression and activity of nitrate transporters and assimilatory enzymes are under complex regulation by mechanisms that are not yet fully understood. Experiments were designed to investigate the relationships between cytosolic ion activity changes and the regulation of nitrate transporters and assimilatory enzymes.Two different approaches were used. Firstly, cytosolic ion activity changes were measured under light-dark transitions, treatment known to affect the regulation of nitrate transporters and assimilatory enzymes. These changes were initially studied using ion-selective microelectrodes. Light-dark transitions were shown to cause changes in cytosolic nitrate activity in mesophyll cells. Dark treatment caused an increase in cytosolic nitrate activity, light treatment a decrease. Plants without functional nitrate reductase (NR) did not show such changes and a higher constant level of cytosolic nitrate activity was observed compared with wild type, suggesting that NR activity has a role in cytosolic nitrate activity changes. These results are consistent with a signalling role for cytosolic nitrate activity in the regulation of nitrate transporters and assimilatory enzymes. The role of NR in influencing cytosolic pH was investigated using pH-selective microelectrodes. The microelectrode measurements were extremely difficult and time consuming so as an alternative pH-indicator dyes were tested but were not distributed in the cytosol of mesophyll cells.The second approach involved the use of nitrate-inducible luciferase reporter plants. A photomultiplier tube/fibre optic cable system was developed and used to quantify luminescence from these plants. A low-light imaging camera was also used. Both systems showed that the reporter plants provided were unsuitable for the study of nitrate-induction. Alternative nitrate-inducible reporter plants were produced, which were shown to be nitrate-inducible and suitable for nitrate-induction studies.The results presented provide supporting evidence for the hypothesis that NR-dependent changes in cytosolic nitrate activity may function as signals in Arabidopsis mesophyll cells.</p
Visualising xylem vessels connections formed one year after grafting with different techniques
No full textGrapevine grafting is an important technique in viticulture and the formation of vascular connections between the scion and rootstock is essential for successful grafting. This study investigates the three-dimensional (3D) structure of the graft interface produced by commonly used techniques, namely, the omega, full cleft, cleft, and Whip and Tongue.
X-ray micro-computed tomography was used to determine anatomy of the graft union based on tissue x-ray density and functional xylem vessels were labelled with the contrast agent. The total volume of the graft interface, the volume of air spaces/necrotic tissues and the distribution of functional xylem vessels was studied.
Functional xylem vessels connect the scion and the rootstock in all grafting types, but in some grafts, this connection is not continuous around the entire circumference of the graft interface. We found that the proportion of air spaces/necrotic tissues relative to the graft interface volume was not affected by grafting type. Omega grafts were found to be more compact, with shorter graft interfaces and as a consequence a smaller absolute volume of air spaces/necrotic tissues at the graft interface.
By combining 3D morphological and functional analysis, this study presents a step forward in addressing the questions facing modern viticulture
The 'true use of reading' : Sarah Fielding and mid eighteenth-century literary strategies.
No full textPhDThe aim of this thesis is to explore, by examining her life and
works, how Sarah Fielding (1710-68) established her identity as an author.
The definition of her role involves her notions of the functions of
writing and reading.
Sarah Fielding attempts to invite readers to form a sense of ties
by tacit understanding of her messages. As she believes that a work
of literature is produced through collaboration between the writer and
the reader, it is an important task in her view to show her attentiveness
toward reading practice. In her consideration of reading, she has two
distinct, even opposite views of her audience: on the one hand a familiar
and limited circle of readers with shared moral and cultural values and
on the other potential readers among the unknown mass of people. The
dual targets direct her to devise various strategies. She tries to
appeal to those who can endorse and appreciate her moral values as well
as her learning. Her writings and letters testify that she is sensitive
to the demands of the literary market, trying to lead the taste of readers
by inventing new forms.
The thesis opens with an overview of Sarah Fielding's career,
followed by a consideration of her critical attention to the roles of
reading. I go on to examine the narrative structures and strategies
she deploys, with a particular emphasis on her use of the epistolary
method. The following chapter deals with her attention to the reading
of the moral message tangibly embodied in her educational writing. It
is followed by an analysis of the activity which earned her a reputation
as a learned woman. Various as the forms of her works are, they invariably
reflect her attempt to balance herself between the two demands of
inventiveness and familiarity
First person - Sarah Alghamdi
No full textABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Sarah Alghamdi is first author on ‘ Contribution of model organism phenotypes to the computational identification of human disease genes’, published in DMM. Sarah is a PhD student in the lab of Robert Hoehndorf at King Abdullah University of Science and Technology, Thuwal, Saudi Arabia, investigating artificial intelligence, specifically knowledge representation and reasoning over biomedical data
Portrait of the English anthropologist Gregory Bateson, New Guinea, 1929 [picture] /
No full textPart of the collection: Sarah Chinnery photographic collection of New Guinea, England and Australia.; Gregory Bateson, famous English anthropologist, New Guinea research in Bainings and Sepik, eventually lived and worked in the United States. Author of "Naven" and other works. -- Accompanying notes from family.; Inscription: "1929" -- On label. "Gregory Bateson, 'Naven' and other works" -- In red ink.; Sarah Chinnery no.: Part 2.; Also available in an electronic version via the internet at: http://nla.gov.au/nla.pic-vn4506462
Portrait of the anthropologist Professor Hortense Powdermaker from Queens, New York, in New Guinea, 1929 [picture] /
No full textPart of the collection: Sarah Chinnery photographic collection of New Guinea, England and Australia.; Inscriptions: "Professor Hortense Powdermaker, (Queens N.Y., U.S.A.) 'Life in Lesso [i.e. Lesu]' and other works" --In red ink. "1929" -- In pencil.; Professor Hortense Powdermaker, American anthropologist 1929 research in Lesu, New Ireland, New Guinea. Author of "Life in Lesu" and other works. -- Accompanying notes from family.; Sarah Chinnery no.: Part 2.; Also available in an electronic version via the internet at: http://nla.gov.au/nla.pic-vn4506463
Portrait of Bill Harney the "Keeper of Uluru", Black Rock, Victoria, ca. 1955, 3 [picture] /
No full textPart of the collection: Sarah Chinnery photographic collection of New Guinea, England and Australia.; Bill Harney, Patrol Officer, Northern Territory. Later was keeper of Uluru, poet, author, at Chinnery's Black Rock home. -- Accompanying notes from family.; Condition: Scratched.; Also available in an electronic version via the internet at: http://nla.gov.au/nla.pic-vn4554174
Sarah Fielding: Satire and Subversion in the Eighteenth-Century Novel
No full textThis study of Sarah Fielding (1710―68) is an original contribution to Fielding scholarship that has a dual purpose: to support those who are striving to re-introduce her to the modern literary landscape in an effort to restore her eighteenth-century literary standing, and to firmly establish Fielding as an early feminist writer. It is argued here that throughout her oeuvre Fielding challenged prevailing traditions that denied women a choice, particularly in education, employment and marriage. These themes are also considered in the political treatises of Mary Astell (1666―1731) and Mary Wollstonecraft (1759―97), who are now widely recognised as feminist writers.
It is further argued that Fielding’s subversion in fiction of the English patriarchal system is underscored by her unorthodox performance in the literary arena. This is fully explored alongside her use of sentimentalism as a literary tool with which she challenges her seemingly inhumane society. Fielding’s interest in ‘the Labyrinths of the Mind’ (in modern terms, human psychology) will also be addressed as will her placement in the history of feminism and her placement in the sentimental novel tradition. Fielding’s performance as a literary critic will be compared with the few female authors who, like her, dared to publish literary criticism during her writing career. Accordingly, extracts from Fielding’s novels and her two critical pamphlets will be thoroughly examined.
An updated biography of Fielding that is also included here will provide evidence for a further claim, that her fiction is autobiographical in part. A comprehensive account of Fielding’s performance as a literary critic forms the final chapter of this work. It is the first full-length examination of her contribution to the genre and includes an appraisal of her recently unearthed critical pamphlet entitled A Comparison Between the Horace of Corneille and The Roman Father of Mr. Whitehead (1750) that is yet to be formerly attributed to her. Ultimately this study of Fielding will go far beyond what has previously been written about this remarkable eighteenth-century author, particularly regarding her feminist activity
Untargeted metabolomics raw data
No full textSemi-polar compounds were extracted, including primary and secondary metabolites, using automated high-throughput ethanol extraction procedures at the MetaboHUB-Bordeaux Metabolome (https://metabolome.u-bordeaux.fr/) from 35 mg of fresh powder, following previously established protocols (Luna et al., 2020). All samples were randomised and injected alternately with extraction blanks (prepared without plant material and used to rule out potential contaminants detected by untargeted metabolomics), and 13 Quality control samples that were prepared by mixing 10 µL from each sample. Quality control samples were injected every 8 runs and used for the correction of signal drift during the analytical batch, and the calculation of coefficients of variation for each metabolomic feature so only the most robust ones are retained for chemometrics (Broadhurst et al., 2018).
Untargeted analysis was performed on a UHPLC Vanquish (Thermo Fisher Scientific) coupled to a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific). One µL of sample was injected on a Phenomenex Luna® Omega Polar C18 column (50 x 2.1 mm, 1.6 µm) at 40°C and a gradient of solvent A (milliQ water – 0.1 % formic acid) and solvent B (acetonitrile – 0.1% formic acid) with a flow of 0.5 mL min-1 was used. The gradient elution was set as follows: 0-11.5 min: 1-40% solvent B; 11.5-12.5 min: 40-95% solvent B; 12.5-14 min: 95% solvent B; 14.5-16 min: 1% solvent B.
The mass spectrometry data was acquired in negative polarity at 140.000 FWHM resolution with an automatic gain target at 3e6 and maximum IT of 100 ms. The source conditions were as follow: Spray voltage: 3000 V; Sheath gas: 45 a.u; Auxiliary gas: 15 a.u; Capillary temperature: 320°C; Probe heater temperature: 250°C; S-lens RF level: 100. The experiments were in full scan (mass range: 70-1050 m/z) – data depending MS2 with top three precursors and normalized collision energies of 15, 30, 45 using a dynamic exclusion of 5 s
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