1,721,006 research outputs found
Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa.
No full textOdorant binding proteins (OBP) are soluble lipocalins produced in large amounts in the nasal mucosa of several mammalian species. Although OBPs can bind a large variety of odorous compounds, direct and exclusive involvement of these proteins in olfactory perception has not been clearly demonstrated. This study investigated the binding properties and chemical resistance of OBP to the chemically reactive lipid peroxidation end-product 4-hydroxy-2-nonenal (HNE), in an attempt to establish a functional relationship between this protein and the molecular mechanisms combating free radical cellular damage. Experiments were carried out on recombinant porcine and bovine OBPs and results showed that both forms were able to bind HNE with affinities comparable with those of typical OBP ligands (K(d) = 4.9 and 9.0 microm for porcine and bovine OBP, respectively). Furthermore, OBP functionality, as determined by measuring the binding of the fluorescent ligand 1-aminoanthracene, was partially lost only when incubating HNE levels and exposure time to HNE exceeded physiological values in nasal mucosa. Finally, preliminary experiments in a simplified model resembling nasal epithelium showed that extracellular OBP can preserve the viability of an epithelial cell line derived from bovine turbinates exposed to toxic amounts of the aldehyde. These results suggest that OBP, which is expressed at millimolar levels, might reduce HNE toxicity by removing from the nasal mucus a significant fraction of the aldehyde that is produced as a consequence of direct exposure to the oxygen present in inhaled air
Preparazione di differenti resine di affinità per la purificazione del recettore del complemento di tipo 1 (CR1) da piastrine di coniglio e neutrofili polimorfonucleati.
No full textPlatelet Lysate Promotes in Vitro proliferation of Equine Mesenchymal Stem Cells and Tenocytes
No full textAn innovative bovine odorant binding protein-based filtering cartridge for the removal of triazine herbicides from water
No full textOdorant binding protein (OBP) is a multi-functional scavenger for small hydrophobic molecules dissolved in the mucus lining the nasal epithelia of mammals, characterized by broad ligand binding specificity towards a large number of structurally unrelated natural and synthetic molecules of different chemical classes. Here, we demonstrate for the first time the application of OBP as the active element of an innovative filtering matrix for the removal of environmental pollutants such as triazine herbicides from water samples. The filtering device, obtained by coupling histidine-tagged bovine OBP to a nickel nitrilotriacetic acid (Ni-NTA) agarose resin, was characterized in terms of retention capacity for the herbicides atrazine, simazine, and propazine. Analysis of these herbicides at trace levels with solid-phase microextraction followed by gas chromatography-mass spectrometry using the selected ion monitoring mode proved the capabilities of the proposed device for the decontamination of surface and groundwater samples in the 0.2-2,300 μg/L concentration range, obtaining a reduction in the triazine content greater than 97 %, thus suggesting its possible use for the potabilization of water
A novel ventilated thermoplastic mesh bandage for post-operative management of large soft tissue defects: a case series of three dogs treated with autologous platelet concentrates
Full text linkA ventilated thermoplastic mesh bandage was used for the post-operative management
of large soft tissue defects in three dogs. Once the granulation tissue appeared, the
wounds were treated with liquid or jellified autologous platelet concentrates, Platelet
Rich Plasma (PRP) and Platelet Lysate (PL), to improve the wound healing process.
After cleaning the wound with sterile physiological solution, a dressing was performed
with several layers of cotton. A window through the layers of cotton was opened above
the wound. Then, the platelet concentrate was topically applied, and the bandage was
completed by placing, over the access window, a ventilated thermoplasticmeshmodeled
according to the size and shape of the wound. After 24 h, it was replaced by a low
adhesion bandage. The thermoplastic mesh avoids the direct contact between the
wound and the external layers of the bandage, preventing the drainage of the topical
agent and the removal of the growing healthy granulation tissue. The bandage proposed
in this study is easily applied by the veterinarian and well-tolerated by the animal, ensuring
high welfare standards in stressed patients presenting compromised clinical conditions
Deswapping bovine odorant binding protein.
No full textThe X-ray structure of bovine Odorant Binding Protein (bOBP) revealed its association as a domain swapped dimer. bOBP, devoid of any cysteines, contrasts with other mammalian OBPs, which are monomeric and possess at least one disulfide bridge. We have produced a mutant of bOBP in which a glycine residue was inserted after position 121. This mutation yielded a monomeric bOBP-121Gly+ in which domain swapping has been reverted. Here, we have subsequently introduced two mutations, Trp64Cys and His155Cys, in view to stabilize the putative monomer with a disulfide bridge. We have determined the crystal structure of this triple mutant at 1.65 A resolution. The mutant protein is monomeric, stabilized by a disulfide bridge between Trp64Cys and His155Cys, with a backbone superimposable to that of native bOBP, with the exception of the hinge and of the 10 residues at the C-terminus. bOBP triple mutant binds 1-amino-anthracene, 1-octen-3-ol (bOBP co-purified ligand) and other ligands with microM Kd values comparable to those of the swapped dimer
Il lisato piastrinico promuove la replicazione in-vitro delle cellule staminali mesenchimali e dei tenociti di cavallo.
No full textSPECT/CT imaging for the evaluation of the differentiation efficacy of canine adipose tissue derived mesenchymal stem cells (cAD-MSC) into osteogenic lineage
No full textThe purpose of our study was to isolate, amplify and characterize adipose-derived MSC from Beagle dogs, with a particular analysis of their osteogenic differentiation potential. SPECT/CT imaging was performed to observe the ability of cAD-MSC to promote ectopic bone formation on a three-dimensional scaffold.
cAD-MSCs were harvested from subcutaneous and visceral fat of Beagle dogs. Histological and phenotypic characterization was made before in vivo studies. Cells from young donors with the highest proliferative and osteogenic potential were chosen to evaluate in-vivo behavior of the cAD-MSC towards osteogenic differentiation. Each applied chitosan gelatin scaffold cross-linked with glutaraldehyde contained 2x105 cAD-MSC. After 2 days proliferation in regular medium and one week in osteomedium, scaffolds were implanted in 10 nude mice, 5-6 weeks old divided in 2 groups used for imaging studies. Ali mice received scaffold containing osteoinduced cells on the right side. The 5 mice from the second group were implanted also with an empty scaffold on the left side. The SPECT/CT whole body images were performed after two months. 80 Bq (100 μI) Tc-99m-MDP were injected into the tail vein of the mice. Two hours later imaging was made by laboratory SPECT/CT hybrid camera (nanoSPECT/CT, Mediso Ltd, Hungary).
SPECT/CT analysis of the dorsal region of mice containing an osteoinduced cAD-MSC-seeded scaffold showed Tc-99m-MDP uptake without exceptions. Bone-like structure was evidenced in the CT slices. On the contrary Tc-99m-MDP accumulation was not present in the empty scaffolds in the control mice. Histological analysis by von Kossa staining showed high mineral concentration in the osteoinduced cAD-MSC scaffolds.
Isolated cAD-MSC have osteogenic potential and have a possible application for the treatment of bone diseases in veterinary medicine. Our results demonstrate that direct in vivo imaging with Tc-99m-MDP is a valid noninvasive method for the evaluation of new bone formation in tissue engineering as it is adsorbed preferentially by newly formed minerals and for clinical studies to determine the bone formation dynamics
AGENTI ANTIBATTERICI E ANTIMICOTICI ED USI DERIVATI
No full textLa presente invenzione riguarda l’uso della proteina OBP (Odorant Binding Protein) e/o sue
varianti o di una composizione che le comprende per inattivare/inibire in un individuo il sistema di
comunicazione batterico Quorum Sensing (QS) mediante il binding/sequestramento di almeno una
Molecola Quorum Sensing (QSM).
L’uso secondo la presente è particolarmente utile nel trattamento e/o nella prevenzione delle
condizioni patologiche provocate dai batteri
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