146 research outputs found
Adicion de la figura de la adopcion internacional en el Codigo civil del estado de Guerrero
No full textOverlapping and specialized roles of tomato phytoene synthases in carotenoid and abscisic acid production
No full textCarotenoids are plastidial isoprenoids required for photoprotection and phytohormone production in all plants. In tomato (Solanum lycopersicum), carotenoids also provide color to flowers and ripe fruit. Phytoene synthase (PSY) catalyzes the first and main flux-controlling step of the carotenoid pathway. Three genes encoding PSY isoforms are present in tomato, PSY1 to PSY3. Mutants have shown that PSY1 is the isoform providing carotenoids for fruit pigmentation, but it is dispensable in photosynthetic tissues. No mutants are available for PSY2 or PSY3, but their expression profiles suggest a main role for PSY2 in leaves and PSY3 in roots. To further investigate isoform specialization with genetic tools, we created gene-edited lines defective in PSY1 and PSY2 in the MicroTom background. The albino phenotype of lines lacking both PSY1 and PSY2 confirmed that PSY3 does not contribute to carotenoid biosynthesis in shoot tissues. Our work further showed that carotenoid production in tomato shoots relies on both PSY1 and PSY2 but with different contributions in different tissues. PSY2 is the main isoform for carotenoid biosynthesis in leaf chloroplasts, but PSY1 is also important in response to high light. PSY2 also contributes to carotenoid production in flower petals and, to a lesser extent, fruit chromoplasts. Most interestingly, our results demonstrate that fruit growth is controlled by abscisic acid (ABA) specifically produced in the pericarp from PSY1-derived carotenoid precursors, whereas PSY2 is the main isoform associated with ABA synthesis in seeds and salt-stressed roots.This work was funded by grants from Spanish Agencia Estatal de Investigación MCIN/AEI /10.13039/501100011033 and European Commission NextGeneration EU/PRTR and PRIMA programs to M.R.-C. (PID2020-115810GB-I00 and UToPIQ-PCI2021-121941). M.R.-C. is also supported by Consejo Superior de Investigaciones Cientificas (202040E299) and Generalitat Valenciana (PROMETEU/2021/056 and AGROALNEXT/2022/067). M.E. and E.B.-E. received predoctoral fellowships from MCIN/AEI (BES-2017-080652) and Colombia’s Colciencias Doctorado Exterior program (MINCIENCIAS885/2020), respectively.Peer reviewe
A new approach and gold standard toward author disambiguation in MEDLINE
No full textAuthor-centric analyses of fast-growing biomedical reference databases are challenging due to author ambiguity. This problem has been mainly addressed through author disambiguation using supervised machine-learning algorithms. Such algorithms, however, require adequately designed gold standards that reflect the reference database properly. In this study we used MEDLINE to build the first unbiased gold standard in a reference database and improve over the existing state of the art in author disambiguation. Following a new corpus design method, publication pairs randomly picked from MEDLINE were evaluated by both crowdsourcing and expert curators. Because the latter showed higher accuracy than crowdsourcing, expert curators were tasked to create a full corpus. The corpus was then used to explore new features that could improve state-of-the-art author disambiguation algorithms that would not have been discoverable with previously existing gold standards. We created a gold standard based on 1900 publication pairs that shows close similarity to MEDLINE in terms of chronological distribution and information completeness. A machine-learning algorithm that includes new features related to the ethnic origin of authors showed significant improvements over the current state of the art and demonstrates the necessity of realistic gold standards to further develop effective author disambiguation algorithms. An unbiased gold standard can give a more accurate picture of the status of author disambiguation research and help in the discovery of new features for machine learning. The principles and methods shown here can be applied to other reference databases beyond MEDLINE
High versus low intensity active music therapy in patients hospitalized at the Montserrat clinic
No full textIntroducción:
La musicoterapia es una intervención clínica basada en evidencia, con eficacia en la reducción de síntomas como ansiedad, depresión y modulación de procesos neuroquímicos que promueven el bienestar. Con estudios en los que pasa desapercibido el rol que podría jugar la intensidad de sesiones, en estos efectos. Especialmente en población hospitalizada, teniendo que una estadía limitada llevaría a buscar la mejor concentración posible de sesiones.
Materiales y métodos:
Se realizó un experimento clínico aleatorizado con dos grupos de intervención: alta y baja frecuencia de musicoterapias semanales. La muestra incluyó pacientes hospitalizados en la Clínica Montserrat que cumplieron con criterios de inclusión y exclusión. La asignación a los grupos se realizó de manera aleatoria y las mediciones se realizaron utilizando escalas de síntomas ansiosos, depresivo y estrés, cuestionarios de sensaciones a la musicoterapia y una pregunta sobre la satisfacción de vida.
Resultados:
Ambos grupos resultaron comparables tras la aleatorización y ambos presentaron mejoras significativas en ansiedad, depresión y satisfacción con la vida después de la intervención. De estos, los de alta frecuencia mostraron una reducción significativa en los niveles de estrés en comparación los de baja frecuencia (p<0.05). Finalmente, observando que las expectativas positivas hacia la musicoterapia se asociaron a una mayor reducción de síntomas.
Discusión:
En el análisis de los resultados, se encontró que ambos grupos (alta y baja frecuencia) no presentaron diferencias significativas en las mediciones iniciales de satisfacción de vida, malestar emocional (DASS-21) ni sensaciones relacionadas con la musicoterapia (CISMA). Sin embargo, tras las intervenciones, el grupo de alta frecuencia mostró una disminución significativa en los niveles de estrés (p=0,027, r=0,26). Además, se observaron correlaciones positivas entre una mayor expectativa hacia la musicoterapia y mejores resultados en satisfacción de vida, puntajes totales de DASS-21 y sensaciones positivas (CISMA).
Este estudio demuestra que la musicoterapia activa, especialmente con una alta frecuencia de sesiones, es una intervención eficaz para reducir el estrés y mejorar el bienestar emocional en pacientes hospitalizados con enfermedad mental. Los resultados resaltan la importancia de considerar la frecuencia de la musicoterapia al diseñar programas de intervención en el contexto hospitalario, y sugieren que una mayor frecuencia de sesiones podría ser beneficiosa para los pacientes.Instituto Colombiano del Sistema Nervioso Clinica MontserratEspecialista en PsiquiatríaEspecializaciónIntroduction:
Music therapy is an evidence-based clinical intervention, effective in reducing symptoms such as anxiety, depression and modulating neurochemical processes that promote well-being. Studies have overlooked the role that session intensity could play in these effects. Especially in the hospitalized population, having a limited stay would lead to seeking the best possible concentration of sessions.
Materials and methods:
A randomized clinical experiment was conducted with two intervention groups: high and low frequency of weekly music therapies. The sample included patients hospitalized at the Montserrat Clinic who met inclusion and exclusion criteria. Group assignment was randomized and measurements were made using scales of anxiety, depression and stress symptoms, questionnaires of sensations of music therapy and a question about life satisfaction.
Results:
Both groups were comparable after randomization and both showed significant improvements in anxiety, depression and life satisfaction after the intervention. Of these, high-frequency participants showed a significant reduction in stress levels compared to low-frequency participants (p<0.05). Finally, positive expectations towards music therapy were associated with a greater reduction in symptoms.
Discussion:
In the analysis of the results, it was found that both groups (high and low frequency) did not present significant differences in the initial measurements of life satisfaction, emotional distress (DASS-21) or sensations related to music therapy (CISMA). However, after the interventions, the high-frequency group showed a significant decrease in stress levels (p=0.027, r=0.26). In addition, positive correlations were observed between a higher expectation towards music therapy and better results in life satisfaction, total DASS-21 scores and positive sensations (CISMA).
This study demonstrates that active music therapy, especially with a high frequency of sessions, is an effective intervention to reduce stress and improve emotional well-being in hospitalized patients with mental illness. The results highlight the importance of considering the frequency of music therapy when designing intervention programs in the hospital context, and suggest that a higher frequency of sessions could be beneficial for patients
El mundo alucinante como pastiche: mar y chachachá
No full textLa crítica literaria ha caracterizado al cubano Reinaldo Arenas principalmente como un escritor paródico. Esta categoría resulta pertinente para analizar el carácter intertextual de novelas como El mundo alucinante (1967), reescritura de las Memorias de Servando Teresa de Mier. La parodia, por su parte, suele ser entendida como una reescritura que tiene un objetivo definido y una intención, siempre política y clara. Sin embargo, el énfasis exclusivo en lo paródico ha desviado la atención de la crítica de un rasgo estético igualmente decisivo: la aparente gratuidad transversal en su obra. Esta monografía propone leer El mundo alucinante desde el concepto de pastiche, procedimiento de hipertextualidad comúnmente pensado como menor a la parodia, por ser un tipo de reescritura de ‘puro divertimento’ y aparente sin motivo. El trabajo de grado examina aquellas instancias en las que la narrativa incomoda por sus excesos, detalles “que sobran”, personas “sin función” y palabras repetidas hasta el exceso. La literatura de Arenas está llena de gratuidades, cuyo fin podemos entender con las palabras de José Lezama Lima; es un “placer aristocrático de desagradar”. Tal perspectiva llena un vacío crítico al reconocer a Arenas desde un aparato crítico-teórico que no busca su valor, casi mercantil, ni una lógica de productividad y servicio en un autor como Reinaldo Arenas, quien encarnó la improductividad: un hombre sexualmente improductivo, un cubano antirrevolucionario, un poeta que se apoya en la provocación gratuita y la risa del sinsentido. El autor tuvo una posición marginal frente a un régimen que exige fuerza y reproducción revolucionaria. Así, la monografía se propone a utilizar las metáforas del mar y el chachachá como los procedimientos de transgresión textual y exceso –pastiche– en El mundo alucinante.Literary critics have mainly characterized Cuban author Reinaldo Arenas as a parodic writer. This category is relevant for analyzing the intertextual nature of novels such as El mundo alucinante (1967), a rewriting of Memorias by Servando Teresa de Mier. Parody, for its part, is usually understood as a rewriting that has a defined objective and intention, always political and clear. However, the exclusive emphasis on the parodic has diverted the attention of critics from an equally decisive aesthetic feature: the apparent gratuitousness that runs through his work. This monograph proposes reading El mundo alucinante from the concept of pastiche, a procedure of hypertextuality commonly thought of as inferior to parody, as it is a type of rewriting for ‘pure entertainment’ and apparently without motive. The thesis examines those instances in which the narrative is uncomfortable due to its excesses, “superfluous” details, “functionless” characters, and words “repeated to excess”. Arenas's literature is full of gratuitousness, the purpose of which we can understand in the words of José Lezama Lima: it is an “aristocratic pleasure of displeasing”. This perspective fills a critical void by recognizing Arenas from a critical-theoretical apparatus that does not seek his almost commercial value, nor a logic of productivity and service in an author like Reinaldo Arenas, who embodied unproductivity: a sexually unproductive man, an anti-revolutionary Cuban, a poet who relies on gratuitous provocation and the laughter of nonsense. The author had a marginal position in the face of a regime that demanded strength and revolutionary reproduction. Thus, the monograph proposes to use the metaphors of the sea and the cha-cha-cha as procedures of textual transgression and excess—pastiche—in El mundo alucinante.Pregrad
Perspectives of the River Plate around the time of Rosas : an analysis based upon the personal correspondence, private memoirs and published accounts of British settlers, as well as works by creole authors
No full textThis thesis draws inspiration from the emergence of cultural studies as an academic
pursuit, in addition to the current renewal of interest in the relationship between
literary works and their socio-cultural milieux, to bring together an assortment of
textual traces pertaining to the River Plate around the era of Juan Manuel de Rosas,
governor of Buenos Aires and de facto dictator of Argentina for most of the period
1829-1852. The main texts analysed range from private documents relating to two
Scottish settler families, through accounts published by British citizens with first-hand
knowledge of the region (Un inglés, Cinco años en Buenos Aires and
Beaumont, Travels in Buenos Ayres and the Adjacent Provinces), to three influential
pieces of early Argentinian literature (Echeverria's El matadero, Mármol's Amalia
and Sarmiento's Facundo). One justification of this apparently eclectic approach lies
in the prominence accorded to the incomer in the thought of liberal Platine
intellectuals, a concern evinced in their literary production.
The methodology involves examining the representation of certain
fundamental topics across this range of written artefacts, observing frequent points of
thematic convergence amongst the various texts. In this fashion, I construct an image
of the River Plate region around the Rosas period, whilst also appraising the degree
to which early British settlers matched the idealized notion of the immigrant present
in liberal creole writings.
The study is divided into four main chapters, supplemented by an
introduction, conclusion and appendix. The first chapter summarizes the historical
context of the young Platine republics; the second deals with the themes of society,
community and family, the third focuses upon religion; the fourth considers
perspectives of politics, dictatorship and civil war. The appendix consists of an
unpublished settler autobiography, a remarkable account of the tribulations faced on
a daily basis in the developing Argentina
Revising identification: fairy tales that transform tradition from within
No full textThis dissertation examines contemporary revisions of four classic fairy tales: “Snow White,” “Sleeping Beauty,” “Little Red Riding Hood,” and “Bluebeard,” drawing on José Esteban Muñoz’s concept of “disidentification” to describe the revisionary work they perform. Muñoz provides a model of subversion that illuminates the work done in these revisions, which both relish the pleasure to be found in the fantasy of fairy tales and work to short circuit their anachronistic ideology, to deconstruct and rewrite their heteronormative scripts. The author argues that fairy-tale revisions can transform the genre from within through a careful dance of adherence to and deviation from its formula. Their work is both formal and thematic, a subversion of fairy-tale form and of sexual politics. Because the fairy tale operates largely by encouraging identification (especially in young audiences) with certain roles, producing social conformity, these radical revisions resist social and sexual prescription and in so doing embrace and empower a range of gender and sexual identities.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2018-12-01The student, Esther Dettmar, accepted the attached license on 2016-11-15 at 19:12.The student, Esther Dettmar, submitted this Dissertation for approval on 2016-11-17 at 10:51.This Dissertation was approved for publication on 2016-11-17 at 13:24.DSpace SAF Submission Ingestion Package generated from Vireo submission #10251 on 2017-02-28 at 14:36:22Made available in DSpace on 2017-03-01T16:36:48Z (GMT). No. of bitstreams: 3
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Cre-LoxP-regulated expression of monoclonal antibodies driven by an ovalbumin promoter in primary oviduct cells
No full textAbstract Background A promoter capable of driving high-level transgene expression in oviduct cells is important for developing transgenic chickens capable of producing therapeutic proteins, including monoclonal antibodies (mAbs), in the whites of laid eggs. Ovalbumin promoters can be used as oviduct-specific regulatory sequences in transgenic chickens, but their promoter activities are not high, according to previous reports. Results In this study, while using a previously characterized ovalbumin promoter, we attempted to improve the expression level of mAbs using a Cre/loxP-mediated conditional excision system. We constructed a therapeutic mAb expression vector, pBS-DS-hIgG, driven by the CMV and CAG promoters, in which the expression of the heavy and light chains of humanized immunoglobulin G (hIgG) is preceded by two floxed stuffer reporter genes. In the presence of Cre, the stuffer genes were precisely excised and hIgG expression was induced in pBS-DS-hIgG-transfected 293T cells. In chicken oviduct primary culture cells, hIgG was expressed after transfection of pBS-DS-hIgG together with the ovalbumin promoter-driven Cre expression vector. The expression level of hIgG in these cells was increased 40-fold over that induced directly by the ovalbumin promoter. On the other hand, hIgG was not induced by the ovalbumin promoter-driven Cre in chicken embryonic fibroblast cells. Conclusions The Cre/loxP-based system could significantly increase ovalbumin promoter-driven production of proteins of interest, specifically in oviduct cells. This expression system could be useful for producing therapeutic mAbs at high level using transgenic chickens as bioreactors.</p
Specific sets of geranylgeranyl diphosphate synthases and phytoene synthases control the production of carotenoids and ABA in different tomato tissues
No full textPlant carotenoids are plastid-synthesized isoprenoids with roles as photoprotectants, pigments, and precursors of bioactive molecules such as the hormone abscisic acid (ABA). The first step of the carotenoid biosynthesis pathway is the production of phytoene from geranylgeranyl diphosphate (GGPP), catalyzed by phytoene synthase (PSY). GGPP produced by plastidial GGPP synthases (GGPPS) is channeled to the carotenoid pathway by direct interaction of GGPPS and PSY enzymes. Three plastid-localized GGPPS isoforms (referred to as SlG1-3) and three PSY enzymes (PSY1-3) are present in tomato (Solanum lycopersicum). Our previous work showed that SlG1 and PSY3 function together in the roots, whereas the rest of the isoforms are required in aerial tissues. Here we generated and analyzed combinations of double mutants lacking PSY1 or PSY2 and SlG2 or SlG3 to investigate the contribution of specific GGPPS and PSY pairs to the production of carotenoids and ABA in different tissues of the tomato plant. Despite that the loss of individual enzymes was found to trigger compensatory mechanisms that complicate interpretation of the results, the results confirm a major role for SlG3 in providing GGPP to PSY2 for housekeeping carotenoid biosynthesis in leaves, whereas SlG2 and PSY1 become most relevant when a more active production is required in flowers and breaker fruits, i.e., at the onset of ripening. We could also confirm that ABA production in the fruit pericarp is more dependent on PSY1 activity than on total carotenoid levels and that fruit size correlates with ABA levels accumulated in ripe rather than breaker fruits
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