1,721,031 research outputs found

    Post-thaw survival of ram spermatozoa and fertility after insemination as affected by prefreezing sperm concentration and extender composition

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    A study was conducted to investigate the effects of prefreezing sperm concentration using two extenders on post-thaw survival and acrosomal status of ram spermatozoa (Experiment 1) and fertility after intrauterine insemination with differing doses of semen (Experiment 2). In autumn (Northern hemisphere), semen was collected by artificial vagina from 8 adult Leccese rams and ejaculates of good quality semen were pooled. Two extender systems for cryopreservation were considered, one based on milk-lactose egg yolk (Milk-LY) and the other based on tris-fructose egg yolk (Tris-FY). Experiment 1 (2 x 6 factorial scheme) examined the in vitro characteristics of spermatozoa in relation to the Milk-LY and Tris-FY extenders and six prefreezing sperm concentrations (50, 100, 200, 400, 500 and 800 x 10(6) spermatozoa/mL). Experiment 2 (2 x 4 factorial) evaluated the influence of the Milk-LY vs Tris-FY extenders and four doses (20; 40, 80 and 160 x 10(6) spermatozoa/0.25 mL) corresponding to prefreezing spermatozoa concentrations of 100, 200, 400 and 800 x 106 spermatozoa/mL, on fertility of ewes inseminated in uterus by laparoscope. Prefreezing sperm concentration influenced (P <0.01) freezability of spermatozoa and affected negatively all the in vitro parameters at 800 x 106 spermatozoa/mL. Overall, Milk-LY tended to ensure higher viability and acrosomal integrity of spermatozoa after thawing at the intermediate sperm densities (range 100 to 500 x 106 spermatozoa/mL). At 500 x 106 spermatozoa/mL concentration corresponded the best condition for survival of spermatozoa (71.2%), acrosome integrity (71.5%) and acrosomal loss (6.0%). At the lowest sperm concentration (50 x 106 spermatozoa/mL), Tris-FY resulted in a higher survival rate than Milk-LY (61.3%, P < 0.05) and lower acrosomal loss (9.7%, P < 0.05). Milk-LY supported spermatozoa motility better than Tris-FY after incubation at sperm concentration between 50 and 400 x 106 spermatozoa/mL (0.05 > P < 0.01). Semen doses of 20 to 40 x 106 spermatozoa/ewe provided satisfactory fertility rates (64 to 81%). The increase of inseminate doses to 160 x 106 spermatozoa/ewe failed to improve fertility, actually tending to decrease lambing rates. (C) 2001 by Elsevier Science Inc

    Biological Health Markers Associated with Oxidative Stress in Dairy Cows during Lactation Period

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    This review aims to summarize and present different biological health markers in dairy cows during the lactation period. Biochemical health markers provide an indicator of how foreign chemical substances, whether external or internal, affect the animal’s health. To understand the relationship between dairy cow health issues and oxidative stress, various biomarkers of oxidative stress must be investigated. Biochemical and hematological factors play a significant role in determining the biological health markers of animals. A variety of biochemical parameters are dependent on various factors, including the animal’s breed, its age, its development, its pregnancy status, and its production status. When assessing the health of cattle, a blood test is conducted to determine the blood chemistry. To diagnose diseases in dairy animals, the blood biochemistry is necessary to determine the cause of many physiological, metabolic, and pathological problems. Observing blood alterations during pregnancy and at peak lactation may determine what factors lift oxidative stress in cows due to disturbances in feed intake and metabolic processes

    Effect of type of suckling and polyunsaturated fatty acid use on lamb production. 2. Chemical and fatty acid composition of raw and cooked meat

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    This study was carried out in order to examine the chemical and fatty acid composition of raw and cooked meat obtained from lambs raised under mothers or reared by artificial suckling with acidified milk replacers with or without polyunsaturated fatty acid (PUFA) supplementation. Meat samples were taken from twenty Gentile di Puglia male lambs subjected to the following feeding treatments: the control group received only maternal milk (MM, n.=6) while two groups were reared by artificial suckling with an acidified milk replacer (MR, n.=7) or with an acidified milk replacer supplemented with 10 ml/l of a PUFA enriched oil (MR+PUFA, n.=7). Lambs were slaughtered at 45 days of age. After 24 hours of refrigeration at 4 °C, the lumbar region was dissected from each right half-carcass and split into pieces, one of which was used raw while the other was cooked in a ventilated electric oven at 180 °C until an internal temperature of 75 °C was reached. Chemical and fatty acid analysis were performed on raw and cooked meat, while only raw meat was assessed for cholesterol. Cooking losses were also evaluated. Meat obtained from MR+PUFA fed lambs contained more fat (P<0.01) and less protein (P<0.05) than MM lambs. Nursing under mothers increased the total amount of saturated fatty acids (SFA), compared with both the MR group (P<0.05) and to the MR+PUFA one (P<0.01). In MM meat samples, fewer PUFAs (P<0.01) and omega-6 fatty acids (P<0.01) were found in comparison with both MR diets. The highest PUFA/SFA ratio of meat was recorded for the MR+PUFA group (0.27), with statistical differences respect to the MR group (0.21; P<0.05) and to the MM one (0.14; P<0.01). Lambs raised with maternal milk produced meat containing more cholesterol than the MR+PUFA group (85.89 vs 76.26 mg/100 g; P<0.05). The atherogenicity index of meat was higher following natural rearing in comparison with the MR+PUFA treatment (1.34 vs 1.05; P<0.05), while the PCL/PCE ratio was significantly higher in MR+PUFA samples than in both MM and MR ones (0.93 vs 0.77 and 0.76, respectively; P<0.05). Cooking cancelled the differences between treatments with regard to the all the dietetic parameters evaluated. In conclusion, artificial suckling with acidified milk replacers improves some meat quality features. Supplementation of milk replacers with PUFAs, although in a limited way, may improve the dietetic properties of lamb meat

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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