43 research outputs found
Microscopia de epifluorescencia: Filmes de membranas sinaptosomales de Triatoma infestans
No full textEstos experimentos forman parte de la tesis del Dr. Nicolás Colmano y han sido parcialmente publicados en una revista científica. Nicolás Guillermo Colmano. Tesis de Doctorado en Ciencias Biológicas (UNC). Título: “Caracterización biofísica de membranas naturales de insecto y mamífero que contienen R-GABA. Bases para el desarrollo de superficies funcionalizadas”. Colmano, N.G., Sanchez-Borzone ME, Turina A.V. (2022) Effects of Fipronil and surface behavior of neuronal insect and mammalian membranes. BBA-B 1864:183979 Las membranas naturales de sinaptosomas de Triatoma infestans fueron marcadas con DiIC18 y sembradas en la interfase agua aire desde donde fueron transferidas a soportes de vidrio silanizado y observadas por microscopia de epifluorescencia.Fil: Colmano, Guillermo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; ArgentinaFil: Sánchez, Mariela Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentin
Effects of Fipronil and surface behavior of neuronal insect and mammalian membranes
No full textThe effects of Fipronil as well as the comparison between synaptosomal natural membranes (NMs) of mammal (NMBt) and insect (NMTi), that contain the ionotropic receptor GABA, was studied. This is the first report of biophysical studies of insect NMs and one of the first on the effects of Fipronil over membranes. Our result showed that NMTi appears to be more disordered than NMBt, generates more compressible films at the air-water interphase and they both showed a 2D transition in its compression isotherm. Each NM showed different lateral distribution and characteristic domain shapes when observed by DiI-C18 epifluorescence microscopy. Laurdan GP images suggested that in NMTi films, Ld and Ld-Lo phases are evenly abundant whereas in NMBt the Ld-Lo phase predominates. The differences observed might be attributed to differences in NMs lipid composition. The lipophilic insecticide Fipronil penetrated both NMs packed up to bilayer-compatible lateral pressures. The interface rearrangement after πt affected the interaction of Fipronil mainly in NMTi. The capability of the compound to be incorporated in expanded Langmuir films and remain when they are compressed was also observed. Fluorescence microscopy images suggest a disordering effect in NMTi while it ordered NMBt, showing that Fipronil affected the lateral distribution of lipids in NMs. We suggest that changes in order and lipid lateral organization caused by Fipronil might contribute to its mechanism of action. Our experiments evidenced that differences in the physical state of insect and mammal membranes become relevant for the design of new compounds with potential biological activity.Fil: Colmano, Guillermo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; ArgentinaFil: Sánchez, Mariela Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; ArgentinaFil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Química. Cátedra de Química Biológica; Argentin
Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
No full textPhospholipid monolayers are often used to emulate biological membrane behavior and interactions with diverse compounds. Yet frequently, these monolayers consist of a single phospholipid or binary mixtures. These monolayers do not share many similarities with biological membranes, for this reason, the aim of this work is to construct Biomimetic Membranes (BM) and test whether they reproduce the behavior of Natural Membranes (NM) and their interaction with an insecticide of sanitary interest, Fipronil. The NMs were synaptosomal membranes of Bos taurus cerebral cortex (NM Bt) and cerebral ganglia membranes of Triatoma infestans nymphs (NMTi). The BMs were assembled with the major lipid components of their respective MN (BMBt and BMTi). Biomimetic Membranes π-A isotherms do not reflect the same behavior of NMs, they are devoided of transition and exhibit a smoother outline. The collapse pressure (πc) of BMBt was quite similar to that of NMBt, yet πc of BMTi was higher than the one observed in NMTi. The compression modulus of both BMs at π =30 mN/m was higher than the one found in NMs, this means that BMs at that π, are more condensed than the natural ones. The presence of fipronil in the subphase, even at low concentrations, causes an increase of ΔV, more evident in NMs. πcutoff essays showed that penetration of fipronil in NMs is only possible up to 34 mN/m, whereas in BMs this value increases to 50 mN/m in BMBt and 44 mN/m in BMTi, suggesting that the presence of proteins in NMs modifies the interaction of fipronil. Fluorescence anisotropy studies showed that BMs behaved similarly to NMs. Fipronil exerted no effect over DPH and TMA-DPH anisotropy values of NMs, but it did affect BMs. EFM images of transferred monolayers show that BMs allow a more homogeneous distribution of the DilC18 fluorescent probe than NMs, but they both exhibit non-defined condensed domains at π above 30 mN/m. Fipronil exerts changes in the distribution of the fluorescent probe in NMs and BMs and this effect was more noticeable at high π values (30 mN/m or higher). In Summary, even though BMs did not emulate their respective NMs perfectly, many parameters were successfully reproduced, and BMTi reproduced its natural counterpart more accurately.Fil: Colmano, Guillermo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Sánchez, Mariela Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaXLVIII Reunión Anual de la Sociedad Argentina de BiofísicaSan LuisArgentinaSociedad Argentina de Biofísic
El impacto del mercado nacional en los campesinos productores de nopal tunero en San Martín de las Pirámides entre 1980 y 2009
No full textTesis para optar por el grado de licenciatura en Etnología</p
Esclavos negros en la hacienda azucarera de San Nicolas Ayotla, Oaxaca
No full textTesis para obtener el grado de Doctor en Historia y Etnohistori
Simbolismo y culto religioso: Una interpretación sociocultural del ciclo agricola y festivo en San Nicolas Totolapan, Distrito Federal
No full textTesis para obtener el grado de Licenciatura en Etnohistori
"Intellectum agens" in the 14th Century
No full textEn este trabajo se estudian las líneas maestras de interpretación del intelecto agente en el s. XIV. Está dividido en tres partes: Averroísmo (Juan de Jandún, Marsilio de Padua, Pablo de Venecia y un anónimo). Nominalismo (Guillermo de Ockham y Juan de Buridán), eclecticismo (Teodoro Métochite y Pedro de Ailly), y reduccionismo (Nicolás Oresmes). Potencialismo (Gil de Roma, Radolfo Brito, Walter Burley y un anónimo).The «intellectum agens» in 14th century. In this paper we study the agent intellect in the XIV century. This work is divided into 3 different parts: Averroism (loannes de Janduno, Marsilius Paduanus, Paulus of Venice and an anonymous author). Nominalism (Gulielmus of Ockham and loannes Buridanus), eclecticism (Theodorus Metochites and Petrus of Ailly) and reductionism (Nicolas Oresmes). Potencialism (Aegidius Romanus, Radulfus Brito, Walter Burley and an another anonymous author)
Adapting authoritarianism: institutions and co-optation in Egypt and Syria
No full textThis PhD thesis compares Egypt and Syria’s authoritarian political systems. While the tendency in social science political research treats Egypt and Syria as similarly authoritarian, this research emphasizes differences between the two systems with special reference to institutions and co-optation. Rather than reducibly understanding Egypt and Syria as sharing similar histories, institutional arrangements, or ascribing to the oft-repeated convention that “Syria is Egypt but 10 years behind,” this thesis focuses on how events and individual histories shaped each states current institutional strengthens and weaknesses. Specifically, it explains the how varying institutional politicization or de-politicization affects each state’s capabilities for co-opting elite and non-elite individuals.
Beginning with a theoretical framework that considers the limited utility of democratization and transition theoretical approaches, the work underscores the persistence and durability of authoritarianism. Chapter two details the politicized institutional divergence between Egypt and Syria that began in the 1970s. Chapter three and four examines how institutional politicization or de-politicization affects elite and non-elite individual co-optation in Egypt and Syria. Chapter five discusses the study’s general conclusions and theoretical implications.
This thesis’s argument is that Egypt and Syria co-opt elites and non-elites differently because of the varying degrees of institutional politicization in each governance system. Rather than view one country as more politically developed than the other, this work argues that Syria’s political institutions are more politicized than their Egyptian counterparts. Syria’s political arena is, thus, described as politicized-patrimonialism. Syria’s politicized-patrimonial arena produces uneven co-optation of elites and non-elites as they are diffused through competing institutions. Conversely, the Egyptian political arena remains highly personalized as weak institutions and individuals are manipulated and molded according to the president’s ruling clique. This is referred to as personalized-patrimonialism. As a consequence, Egypt’s political establishment demonstrates more flexibility in ad hoc altering and adapting its arena depending on the emergence of crises.
This study’s theoretical implications suggest that, contrary to modernization and democratization theory’s adage that institutions lead to a political development, politicized institutions within a patrimonial order actually hinder regime adaptation because consensus is harder to achieve and maintain. It is within this context that Egypt’s de-politicized institutional framework advantages its top political elite. In this reading of Egyptian and Syrian politics, Egypt’s personalized political arena is more adaptable than Syria’s. These conclusions do not indicate that political reform is a process underway in either state
El intelecto agente en el s. XIV
No full textIn this paper we study the agent intellect in the XIV century. This work is divided into 3 different parts: Averroism (Ioannes de Janduno, Marsilius Paduanus, Paulus ofVenice andan anonymous author). Nominalism (Gulielmus of Ockham and Ioannes Buridanus), eclecticism (Theodorus Metochites and Petrus of Ailly) and reductionism (Nicolas Oresmes). Potencialism (Aegidius Romanus, Radulfus Brito, Walter Burley andan another anonymous author).En este trabajo se estudian las líneas maestras de interpretación del intelecto agente en el s. XIV. Está dividido en tres partes: Averroísmo (Juan de Jandún, Marsilio de Padua, Pablo de Venecia y un anónimo). Nominalismo (Guillermo de Ockham y Juan de Buridán), eclecticismo (Teodoro Métochite y Pedro de Ailly), y reduccionismo (Nicolás Oresmes). Potencialismo (Gil de Roma, Radolfo B1ito, Walter Burley y un anónimo)
Perspectives of the River Plate around the time of Rosas : an analysis based upon the personal correspondence, private memoirs and published accounts of British settlers, as well as works by creole authors
No full textThis thesis draws inspiration from the emergence of cultural studies as an academic
pursuit, in addition to the current renewal of interest in the relationship between
literary works and their socio-cultural milieux, to bring together an assortment of
textual traces pertaining to the River Plate around the era of Juan Manuel de Rosas,
governor of Buenos Aires and de facto dictator of Argentina for most of the period
1829-1852. The main texts analysed range from private documents relating to two
Scottish settler families, through accounts published by British citizens with first-hand
knowledge of the region (Un inglés, Cinco años en Buenos Aires and
Beaumont, Travels in Buenos Ayres and the Adjacent Provinces), to three influential
pieces of early Argentinian literature (Echeverria's El matadero, Mármol's Amalia
and Sarmiento's Facundo). One justification of this apparently eclectic approach lies
in the prominence accorded to the incomer in the thought of liberal Platine
intellectuals, a concern evinced in their literary production.
The methodology involves examining the representation of certain
fundamental topics across this range of written artefacts, observing frequent points of
thematic convergence amongst the various texts. In this fashion, I construct an image
of the River Plate region around the Rosas period, whilst also appraising the degree
to which early British settlers matched the idealized notion of the immigrant present
in liberal creole writings.
The study is divided into four main chapters, supplemented by an
introduction, conclusion and appendix. The first chapter summarizes the historical
context of the young Platine republics; the second deals with the themes of society,
community and family, the third focuses upon religion; the fourth considers
perspectives of politics, dictatorship and civil war. The appendix consists of an
unpublished settler autobiography, a remarkable account of the tribulations faced on
a daily basis in the developing Argentina
