1,720,975 research outputs found
Raman and FTIR imaging of lung tissue: methodology for controlsamples
No full textThe paper describes Raman and FTIR images of normal lung tissue of an infant patient which is a prerequisite to understand the biochemical changes that accompany pediatric lung pathologies and malformations. A Raman image of 3420 spectra was recorded from a 5.7 mm x 6 mm tissue section in mapping mode using 785 nm excitation laser and a step size of 100 mu m. A Fourier transform infrared (FTIR) image of 4096 spectra was recorded from the same sample at a lateral resolution of 63 mu m using a spectrometer coupled to a focal plane array detector. Whereas the analysis of the FTIR image was complicated by dispersion artifacts, the analysis of the Raman image was impaired by intense spectral contributions of hemoglobin which overlap with spectral contributions of other proteins, lipids and nucleic acids. Data analysis procedures were optimized to reduce these effects. A multiplicative signal correction was performed to normalize the spectra within each dataset. Then, pseudo color images were constructed using cluster analyses. The most significant variations within both images were assigned to different concentrations of red blood cells. Furthermore, mucus and blood vessels were identified in the vibrational spectroscopic images. The different sensitivity of Raman and FTIR imaging for lung tissue constituents supports the combined use of both modalities
Meckel's diverticulum masked by a long period of intermittent recurrent subocclusive episodes
No full textMeckel's diverticulum (MD) is the most frequent congenital abnormality of the small bowel and it is often difficult to diagnose. It is usually asymptomatic but approximately 4% are symptomatic with complications such as bleeding, intestinal obstruction, and inflammation. The authors report a case of a 7-year-old boy with a one-year history of recurrent periumbilical colicky pain with associated alimentary vomiting, symptoms erroneously related to a cyclic vomiting syndrome but not to MD. The clinical features and the differential diagnostic methods employed for diagnosis of MD are discussed. © 2009 The WJG Press and Baishideng. All rights reserved
Meckel's diverticulum masked by a long period of intermittent recurrent subocclusive episodes
No full textRaman and FTIR imaging of lung tissue:bronchopulmonary sequestration
No full textBronchopulmonary sequestration (BPS) involves abnormal pulmonary tissue lacking trancheobronchial connection and having a separate vascular supply that arises from the thoracic or abdominal aorta. For the first time, BPS is studied by Raman and Fourier-transform infrared (FTIR) imaging. Cryosections were mounted on calcium fluoride substrates from tissue samples of twoBPS patients.Ramanimages were collected at a step size of 66–100 μmto assess thewhole tissue section, andat a lower step size of 10 μm to resolve details in selected areas. FTIR images were collected at resolutions of 63 and 4 μm per pixel. Data sets were segmented by cluster analyses, and the spectra of each cluster were compared. The spectra revealed higher red blood cell content and lower collagen and lipid content in BPS than in an additional tissue section from the BPS margin
Hepatopulmonary fusion in a newborn. An uncommon intraoperatory finding during right congenital diaphragmatic hernia surgery; case description and review of literature.
No full textHepatic pulmonary fusion is a rare malformation associated with right congenital diaphragmatic hernia (CDH), often only discovered during surgical repair of the defect. Fourteen previous cases have been reported in the literature. We describe a case of a full term male newborn with prenatal ultrasound diagnosis of right CDH who underwent a thoracoscopy converted to a thoracotomy, due to this rare aforementioned intraoperative incidental finding. We reviewed the previous reported literature, especially focusing on the chosen surgical approach, concluding that an early and appropriate preoperative imaging investigation may be crucial for the best management of these kinds of patients
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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