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Glycan profiles of the male donkey urethra
No full textThe urethra of the male has a dual function, acting as a route foe urine and seme
Differential glycopattern in the horse pelvic and penile urethra epithelium
No full textThe urethra of the male has a dual function acting as a route for urine and semen. Often the male urethra corresponds to the preferntial entrance for pathogenic agents that can affect both the...
Rabbit metabolic response and selected meat quality traits: Evaluation of dietary PLX®23 and LycoBeads® feed supplement.
No full textEffects of dietary supplementation of verbascoside and lycopene, alone and in combination, on some blood parameters, plasma oxidative status and meat quality traits in intensively reared rabbits were investigated. The test lasted 60 days and was conducted on 200 weaned-rabbits, divided into four groups of 50 animals each (5 animals each cage × 10 repetitions). The control group (CON) received a fattening feed without any feed supplements, whereas the experimental groups received the following: the first, an integration in 22 g of PLX ® 23 (Lippia citriodora extract; VB group) per ton of feed; the second, an integration in 100 g of LycoBeads ® (Solanum lycopersicum extract; LIC group) per ton of feed; and the third, an integration of both feed supplements in combination at the same doses (LIC+VB group). The dietary supplementation with Lippia citriodora and Solanum lycopersicum resulted in an improvement of the blood lipid profile, oxidative plasma markers, and hepatic and renal activity of treated growing rabbits. Feed additives also produced meat with a lower content in SFA and an increased PUFA content, and inhibited the lipid oxidation improving the oxidative stability of rabbit meat. The dietary supplementation, even reporting no effects on productive parameters and carcass yield, showed an improvement on several blood parameters, as indicator of animal welfare, and on quality and healthy meat markers. © 2018 Blackwell Verlag Gmb
Expression of heat shock proteins 60, 70, 90 in equine granulosa cells
No full textIt is well known that heat shock proteins (HSPs) are not only mediators of heat stress but also regulators of cell proliferation (1) differentiation (2) and apoptosis (3). During the equine..
EXPRESSION OF L-TYPE VOLTAGE CALCIUM CHANNELS IN EQUINE GRANUOSA CELLS: A MOLECULAR AND FUNCTIONAL STUDY
No full textThe ovary is dependent on pituitary gonadotropins to ensure folliculogenesis which in turn relies on intracellular calcium signalling, induced by follicular stimulating hormone to promote oocyte activation and maturation [1]. The intracellular calcium concentration [Ca2+]I rise is due in part to L-voltage-gated calcium channels (L-VOCCs). The role of L-VOCCs in the ovarian follicular dynamic has been assessed in the granulosa cells (GCs) of several animals [2] but not in the mare, whose follicular growth is primarily controlled by the changing day length that drives the reproductive seasonality. This study aims to evaluate the presence, quantification, and localization of L-VOCCs in equine GCs recovered from follicles of different diameters and their involvement in modulating [Ca2+]i during follicles development. GCs were recovered from follicles of ovaries obtained during the year from a local slaughterhouse. An average of 5±1 mares were analyzed each month. Based on the cyclic periodicity, the number of follicles varied. Follicles were categorized as small, medium, and large [3]. GCs from each type of size-follicle were pooled and processed by a) western blot, to assess the expression and quantify L-VOCCs employing a rabbit affinity-purified primary antibody against the α1 subunit of L-VOCCs and a biotinylated universal secondary antibody (Vectastain Elite ABC Universal Kit, Burlingame, USA). The positive hybridization was revealed by a DAB substrate kit (Vector-Laboratories, Burlingame, USA); b) immunohistochemistry, to localize L-VOCCs by the same primary antibody used in the western blot procedure and a FITC-conjugated secondary antibody; c) fluorescent measurement of L-VOCCs activity and cytosolic Ca2+ dynamics employing Fura-2-AM fluorescent dye and Bay k-8644 and Nifedipine, which are agonist and antagonist, respectively, of the L-VOCCs using a QuantiCell 900 integrated image system (VisiTech International, Sunderland, UK). Data were analyzed by parametric tests. Results showed that all categories of GCs expressed L-VOCCs, which, as aspected, appeared as doublet bands at different molecular weights. Fluorescence immunohistochemistry evidenced positive signals at the surface of GCs. The quantification of the L-VOCC level showed that small follicles increased their expression in February (January vs February, P<0.05), just before the beginning of the equine breeding season. Moreover, it has been found that the used agonist and antagonist of the L-VOCCs induced a significant (P<0.001) change in the [Ca2+]i in follicles of all sizes. Large follicles resulted in a greater change in [Ca2+]I after addition of Bayk K-8644 or Nifedipine when compared to the basal condition. The results obtained indicate the existence of a regulatory system of the function of the L-VOCCs associated with the functional state of the follicle during its growth and maturation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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