7,576 research outputs found
Reconstruction of the Original Text of Chang-tzŭ Hsiao-yao-yu-p'ien 荘子逍遥遊篇
Full text linkThe author makes a study into the four fables which are told in the present text of Hsiao-yao-yu-p’ien 逍遥遊篇 of Chuang-tzŭ 荘子. In examining the phraseology, such as the continuation of words, the sequence of themes and the sentence structure, the author notices first of all that in the first fable so many insertions were added to the text by later writers that the original meaning is likely to be misunderstood. Secondly, some parts of the original text of the second and third fables may have been left out in the course of time. Finally, there are considerable number of misplaced sentence in the fourth fable. Consequently, the author believes that the first three fables represent the philosophy of Chuang Chou 荘周 himself, but that the fourth one does not.Lu Tê-ming 陸徳明 wrote in his Ching-tien-shih-wên-hsü-lu 経典釈文序録 that the principal philosophy of Chuang Chou can be explained in four concepts—hsiao-yao 逍遥, tzŭ-jan 自然, wu-wei 無為 and ch’i-wu 斉物. Among 33 extant chapters of Chuang-tzŭ, there are two entitled Hsiao-yao and Ch’i-wu respectively. Bearing these facts in mind, one can assume that the original text of Chuang-tzŭ consisting of 52 chapters would have contained two chapters which were entitled Tzŭ-jan, Wu-wei respectively.In conclusion, the author further assumes that in the original Chuang-tzŭ, the first fable of the present Hsiao-yao-yu belonged to the chapter Hsiao-yao and the second and third fables to the Wu-wei.journal articl
The concept of peace in the Tao Te Ching
Full text linkThis thesis represents a first attempt to analyze Lao Tzu's main method of resolving the social and political problems in Ch'un Ch'iu and Warring States Periods. Lao Tzu, the founder of Taoism, suggested many solutions that could bring an end to the conflicts and to the ending of the disunity of China. Examples of these include the reform or abolishment of some ancient Chinese institutions as well as new principles for the enhancement and preservation of life. All these ideas were to become crystallised in Tao Te Ch'ing, which became an important religious text. In particular, the aspect of Lao Tzu's methods for solving the social and political problems of China in the Warring States Period needs more attention. In each chapter of the Tao Te Ch'ing there was a main emphasis on peace as the main method of developing social cohesion and as a cure to all fundamental human problems. Therefore, Lao Tzu's ideas about peace and his methods of solving the problems of the Warring States period are significant and from the main focus of the thesis
supplemental_Table – Supplemental material for Risk and protective factors for adolescents’ illicit drug use: A population-based study
No full textSupplemental material, supplemental_Table for Risk and protective factors for adolescents’ illicit drug use: A population-based study by Jung-Yu Liao, Chiu-Mieh Huang, Charles Tzu-Chi Lee, Hsiao-Pei Hsu, Chiu-Ching Chang, Che-Jen Chuang and Jong-Long Guo in Health Education Journal</p
Stimulation of GCMa Transcriptional Activity by Cyclic AMP/Protein Kinase A Signaling Is Attributed to CBP-Mediated Acetylation of GCMa
No full textIdentification of HDAC regulating hGCMa activity
No full text 人類胎盤特殊轉錄因子hGCMa，屬於GCM基因家族，在胎盤的發育上扮演重要的角色；hGCMa促進胎盤中細胞滋養層(cytotrophoblast)細胞融合形成融合滋養層(syncytiotrophoblast)，為胎兒的發育所必須。
在實驗室先前研究中發現，在PKA的刺激之下，組蛋白乙醯轉移酶(histone acetyl-transferase，HAT)中的CBP會與hGCMa的結合並促進CBP對hGCMa進行乙醯化。而當hGCMa乙醯化後其蛋白的穩定度與轉錄活性皆上升，必定會誘發下游的訊息傳遞的進行，因而推測應有一個負調控機制存在，對hGCMa進行去乙醯化以降低hGCMa的活性。而在此次研究中發現，加入組蛋白去乙醯酶(histone deacetylase，HDAC)的抑制劑trichostatin A (TSA)後，hGCMa的乙醯化狀態增加且轉錄活性也更為上升。接著更進一步確定了HDACα, HDACγ, HDACκ和HDACλ可與hGCMa相結合而促進hGCMa進行去乙醯化。因為HDACγ呈現了較強的去乙醯化的效果與結合能力，透過進一步的短暫表現(transient expression)的實驗發現了HDACγ可抑制hGCMa的轉錄活性。
上述結果顯示乙醯化的hGCMa可做為數個去乙醯酶(deacetylase)分子的受質(substrate)。本篇報告同時呈現了一個新的機制－－hGCMa的活性透過組蛋白乙醯轉移酶及去乙醯酶兩大類分子而達到後轉譯(posttranslational)層次上的調控。Human GCMa is a zinc-containing transcription factor that plays a critical role for placenta development. hGCMa regulates the fusion of cytotrophoblasts into a syncytiotrophoblast layer, which is indispensable for the proper development of fetus.
In our previous studies, we have demonstrated that PKA stimulates the association of hGCMa with CBP and increases hGCMa acetylation by CBP. Because hGCMa acetylation enhances its stability and transcriptional activity, there should be a negative regulation system, such as a deacetylation mechanism, to reverse hGCMa activity. In the current study, we observed that trichostatin A (TSA), an inhibitor of multiple histone deacetylases (HDACs), enhances the acetylation state of hGCMa and hGCMa-mediated transcriptional activity. We further found that hGCMa can be deacetylated through the interaction with HDACα, HDACγ, HDACκ and HDACλ Because HDACγ displayed the strongest deacetylation effect and binding efficiency, we also demonstrated that HDACγinhibits hGCMa-mediated transcriptional activity in transient expression experiments.
These results suggest that acetylated hGCMa is a substrate for several deacetylases and reveal a new mechanism for the posttranslational regulation of hGCMa activity by histone acetyl-transferase and deacetylases.目 錄 I
圖表目錄 III
縮寫表 IV
中文摘要 VI
英文摘要 Abstract VII
第一章 緒論
1.1 胎盤 1
1.2 gcm轉錄因子基因家族 4
1.3 轉錄因子相關的後轉譯修飾 8
1.4 組蛋白去乙醯酶 (Histone deacetylase) 11
1.5 研究動機及目標 12
第二章 材料及方法
2.1 重組質體的構築 14
2.2 細胞培養 15
2.3 短暫轉染 (Transient transfection) 15
2.4 細胞蛋白萃取及處理 16
2.5 西方墨點法 (Western blotting) 17
2.6 共同免疫沉澱法 (Co-immunoprecipatation) 17
2.7 蛋白表現 18
2.8 GST沈澱試驗 (GST pull-down assay) 19
2.9 螢光報告基因Luciferase活性檢測 (Luciferase assay) 19
2.10 免疫染色 (Immunostaining) 20
第三章 實驗結果
3.1 TSA促進了hGCMa的乙醯化與轉錄活性。 22
3.2 HDAC分子與hGCMa的交互作用及去乙醯化作用。 24
3.3 HDACγ與hGCMa在活體內外的交互作用。 27
3.4 定位HDACγ與hGCMa的結合區域。 29
3.5 HDACγ抑制hGCMa的轉錄活性。 30
第四章 討論與總結 32
第五章 圖表 37
第六章 參考文獻 4
Predicting hospitalization for heat-related illness at the census-tract level: Accuracy of a generic heat vulnerability index in phoenix, Arizona (USA)
No full textabstract: Background: Vulnerability mapping based on vulnerability indices is a pragmatic approach for highlighting the areas in a city where people are at the greatest risk of harm from heat, but the manner in which vulnerability is conceptualized influences the results.
Objectives: We tested a generic national heat-vulnerability index, based on a 10-variable indicator framework, using data on heat-related hospitalizations in Phoenix, Arizona. We also identified potential local risk factors not included in the generic indicators.
Methods: To evaluate the accuracy of the generic index in a city-specific context, we used factor scores, derived from a factor analysis using census tract–level characteristics, as independent variables, and heat hospitalizations (with census tracts categorized as zero-, moderate-, or highincidence) as dependent variables in a multinomial logistic regression model. We also compared the geographical differences between a vulnerability map derived from the generic index and one derived from actual heat-related hospitalizations at the census-tract scale.
Results: We found that the national-indicator framework correctly classified just over half (54%) of census tracts in Phoenix. Compared with all census tracts, high-vulnerability tracts that were misclassified by the index as zero-vulnerability tracts had higher average income and higher proportions of residents with a duration of residency < 5 years.
Conclusion: The generic indicators of vulnerability are useful, but they are sensitive to scale, measurement, and context. Decision makers need to consider the characteristics of their cities to determine how closely vulnerability maps based on generic indicators reflect actual risk of harm.Corresponding Author:
Wen-Ching Chuang
Arizona State University
[email protected]
Adopting the two-branch network to video-text tasks
Full text linkThis Thesis was approved for publication on 2018-04-23 at 16:33.DSpace SAF Submission Ingestion Package generated from Vireo submission #12431 on 2018-08-31 at 17:21:14Made available in DSpace on 2018-09-04T20:36:51Z (GMT). No. of bitstreams: 2
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Previous issue date: 2018-04-23Modeling visual context and its corresponding text description with a joint embedding network has been an effective way to enable cross-modal retrieval. However, while abundant work has been done for image-text tasks, not much exists with regards to the video domain. We hope to adopt a nonlinear embedding model, the two-branch network, to the video-text tasks in order to show its robustness. Two kinds of tasks are explored, bidirectional video-sentence retrieval and video description generation. For the retrieval task, we use nearest neighbor search to get the corresponding video or text with respect to the query. For video captioning, we incorporate the two-branch network in a traditional LSTM model with an additional embedding loss term in order to demonstrate its ability of preserving a semantic structure between video and text.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-05-01The student, Hsiao-Ching Chang, accepted the attached license on 2018-04-23 at 16:08.The student, Hsiao-Ching Chang, submitted this Thesis for approval on 2018-04-23 at 16:14.Embargo set by: Seth Robbins for item 107294
Lift date: 2020-09-04T20:37:00Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 107294
Lift date: 2020-09-04T20:42:08Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 107294 on 2020-09-05T09:15:20Z
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