170 research outputs found
Tobias Nicklas, Friedrich V. Reiterer, Joseph Verheyden (éd.), in coll. with Heike Braun, Deuterocanonical and Cognate Literature. Yearbook 2009. The Human Body in Death and Resurrection, Berlin – New York, Walter de Gruyter, 2009
No full textGrappe Christian. Tobias Nicklas, Friedrich V. Reiterer, Joseph Verheyden (éd.), in coll. with Heike Braun, Deuterocanonical and Cognate Literature. Yearbook 2009. The Human Body in Death and Resurrection, Berlin – New York, Walter de Gruyter, 2009. In: Revue d'histoire et de philosophie religieuses, 90e année n°3, Juillet-Septembre 2010. pp. 395-396
Sapientia Salomonis (Weisheit Salomos). Eingeleitet, übersetzt und mit interpretierenden Essays versehen von Karl-Wilhelm Niebuhr, Walter Ameling, Folker Blischke, Mareike V. Blischke, Alfons Fürst, Rainer Hirsch-Luipold, Heinz-Günther Nesselrath, Maren R. Niehoff und Friedrich Reiterer ; herausgegeben von Karl-Wilhelm Niebuhr, Tübingen, Mohr Siebeck, 2015
No full textGrappe Christian. Sapientia Salomonis (Weisheit Salomos). Eingeleitet, übersetzt und mit interpretierenden Essays versehen von Karl-Wilhelm Niebuhr, Walter Ameling, Folker Blischke, Mareike V. Blischke, Alfons Fürst, Rainer Hirsch-Luipold, Heinz-Günther Nesselrath, Maren R. Niehoff und Friedrich Reiterer ; herausgegeben von Karl-Wilhelm Niebuhr, Tübingen, Mohr Siebeck, 2015. In: Revue d'histoire et de philosophie religieuses, 96e année n°3, Juillet-Septembre 2016. pp. 332-333
TwisterSearch: Supporting Social Search with Tabletop and Mobile Displays
No full textThis demo presents TwisterSearch, a system for co-located and collaborative Web search that was designed in accordance with the canonical model of social search by Evans and Chi (2009). In a first phase before search, participants frame the context of the intended search and thereafter gather initial information requirements on a tabletop. These requirements are then refined through discussion and yield the foundation for the search task itself, which is performed in parallel on multiple mobile displays. These private devices are used to search the Web for evidence files and to populate the visual workspace of the tabletop with them. Moreover, the personal device allows both a highly parallel search as well as a tightly-coupled collaborative search, to enable seamless switching between collective and solitary search activities. In our TwisterSearch demo, participants can have a firsthand experience of these different individual phases of social search
The role of the lectin VIP36 in the early secretory pathway
No full textLectins are of emerging importance for quality control and intracellular transport of glycoproteins in mammalian cells. One of the most prominent lectins involved in intracellular transport is ERGIC-53, which belongs to the family of L-type lectins. ERGIC-53 mediates the ER export of several glycoproteins like cathepsin Z, α1-antitrypsin (α1-AT) or blood coagulation factors. VIP36 belongs to the same family as ERGIC-53, but its cellular function remains poorly understood. VIP36 is a type I membrane protein. It cycles within the early secretory pathway and binds high mannose glycans. In order to gain insight into the function of VIP36 we decided to search for a luminal interaction partner for VIP36.
We used a YFP-protein fragmentation complementation (YFP-PCA) based FACS screen of a human adult liver library to unravel an interaction partner for VIP36. Complementation of YFP is irreversible. Therefore, the YFP-PCA is well suited to detect weak interactions, like those between mammalian lectins and glycoproteins. YFP2-VIP36 was used as the bait in our screen. The human liver library was tagged with YFP1. Our screen identified α1-AT as an interaction partner for VIP36. VIP36 recognized high mannose containing α1-AT, which is consistent with the previously obtained results about the glycan affinity of VIP36. This interaction was increased upon inhibition of complex glycosylation by kifunensine. The complex formed by α1-AT and VIP36 was localized to the Golgi and the ER. α1-AT was previously identified as a cargo for ERGIC-53. Knockdown of ERGIC-53 slowed down α1-AT transport, consistent with a role for ERGIC-53 in ER export of α1-AT. In contrast, knockdown of VIP36 accelerated transport of endogenous α1-AT in HepG2 cells. This effect was specific for α1-AT, as the non-glycosylated protein albumin showed no acceleration in transport. In addition, VIP36 knockdown did not affect general protein secretion. This finding makes it unlikely that VIP36 acts as an anterograde cargo receptor for α1-AT. Further studies on the dynamics of the complex formed by VIP36 and α1-AT revealed that VIP36 recycles α1-AT back to the ER, which argues for a role of VIP36 in post-ER quality control. This notion is further supported by the finding that the chaperone BiP co-immunoprecipitated with the complex of VIP36 and α1-AT. This chaperone was previously described as an interaction partner for VIP36. This argues for a complex consisting of VIP36 and BiP acting together in post-ER quality control to detect misfolded α1-antitrypsin in the Golgi and retrieve it back to the ER.
Apart from searching for an interaction partner, I also determined the effect of depletion of VIP36 on the morphology of the secretory pathway. The rationale behind this is the observation that cargo receptors contribute to the structural integrity of organelles of the secretory pathway. Knockdown of VIP36 had no effect on ER exit sites or on the ERGIC. However, VIP36 knockdown resulted in fragmentation of the Golgi apparatus. The fragmented Golgi was not the consequence of disturbed bidirectional protein transport and not due to effects on microtubules. Knockdown of VIP36 reduced COPI staining on the Golgi. VIP36 is likely to provide COPI binding sites on the Golgi via its cytosolic tail and thereby contribute to Golgi structural integrity. Our results underscore the importance of cargo receptors, not only for intracellular transport within the secretory pathway, but also to maintain the integrity of the secretory pathway itself.
In conclusion, my thesis provides a deeper insight into the function of VIP36 in the early secretory pathway
"A Hetz muaß sein!": Elfriede Jelineks Funktionalisierung und Demontage österreichischer Theater- und Dramentraditionen
No full textAttorialità performative sul confine tra vita e teatro
No full textThis article examines the experiences of four theatre groups: Compagnia Pippo Delbono, Accademia Arte della diversità and Compagnia Babilonia Teatri in Italy, and Theater Hora in Switzerland. The author explores the hypothesis that some of the productions put on by these groups are similar in the way they strive for an authentic uniqueness of performative action, intuitively recognised by visionary directors in the stage presence of disabled actors. The performative and theatrical quality of these groups' actors and production seems to express a synthesis of aesthetic and ethical aspiration that constitutes the essence of theatre. This gives rise to the idea that the theatre is rediscovering its contemporary urgency by breaking through the purely representative, fictional dimension and into a new relationship between life and action on stage, and the possibility of a real transformation of the actors and the audience
IA von Websites: asynchrone Remote-Tests und Laborstudien im Vergleich
No full textDer Beitrag zeigt auf, wie Crowdsourcing-Verfahren für asynchronous remote usability testing eingesetzt werden können. Das konkrete Szenario hierfür ist die Untersuchung der Informationsarchitektur (IA) von Websites mit Hilfe des Tree-Test und des Navigations-Stress-Test. Für beide Methoden wurden Crowdsourcing-kompatible Online-Verfahren entwickelt bzw. angepasst. In einer Vergleichsstudie werden jeweils gleiche Aufgaben sowohl über Crowdsourcing-Plattformen als auch in einem Laborsetting getestet. Diese empirische Studie zeigt, dass sich vergleichbare Ergebnisse erzielen lassen, wobei sich für die Crowdsourcing-basierte Untersuchung mit vergleichsweise geringem Aufwand hinreichend viele Testpersonen rekrutieren lassen. Der Beitrag ist insofern auch im Sinne des network as the extension of the usability laboratory zu verstehen
Hierarchie und Organisationsausgestaltung einer projektorientierten Immobilienorganisation im Zuge der bevorstehenden Pensionierungswelle
No full text"Werde zum virtuellen Pfeil" - Simulation des traditionellen Bogenschießens
No full textIn dieser Demonstration zeigen wir den Virtuellen Pfeil , ein Projekt aus dem Studiengang Medieninformatik der FH Düsseldorf. Ziel ist es, einen Simulator für traditionelles Bogenschießen zu entwickeln, der interessierten Besuchern auf Messen eine glaubwürdige Benutzererfahrung dieser Sportart vermittelt. Gleichzeitig soll jedoch auch eine unterhaltsame Komponente integriert werden. Das System wurde im August 2012 auf einer großen Veranstaltung, dem Ideenpark 2012 demonstriert
Zoomtechniken zur Exploration komplexer Informationsräme am Beispiel "HyperGrid"
No full textDie Quantität, Dimensionalität und Multimedialität heutiger Informationsräume stellt bei der Exploration
und Navigation große Anforderungen an die Gestaltung der Mensch-Computer Interaktion. Zoomable
User Interfaces (ZUIs) bieten dabei neue Möglichkeiten für eine benutzergerechtere Herangehensweise.
Neben einem mathematischen Konzept zur Modellierung der Benutzersicht auf komplexe Informationsräume, soll hier am Beispiel des HyperGrids ein leicht-erlernbares Interaktionskonzept mit semantischem Zoom vorgestellt werden. Dieses erlaubt die Präsentation umfangreicher Informationsräume mit Attributen verschiedenster Datentypen und Modalitäten in variablen Detailgraden. Dabei kann das HyperGrid eine analytische Sichtweise auf den Informationsraum durch eine filter- und sortierbare Tabellenstruktur mit einer interessengeleiteten, browsing-orientierten Vorgehensweise funktional und visuell kombinieren
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