1,618,579 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
CHO-S master cell line generation.
No full text(A) The H11 locus was cleaved, using CRISPR/Cas9, to encourage integration of the landing pad donor. A successful knock-in at the H11 locus generates a master cell line that has two PhiC31 attP sites, and that co-expresses three proteins via a PGK promoter-driven transcript. (B) Genotyping of the 5’-arm and 3’-arm in the CHO-S master cell line. PCR was performed with genomic DNA and specific primer pairs. 1: CHO-S parental cell line with 5’-arm primers; 2: CHO-S master cell line ("4–6") with 5’-arm primers; 3: CHO-S parental cell line with 3’-arm primers; 4: CHO-S master cell line ("4–6") with 3’-arm primers.</p
AEROELASTIC ANALYSIS OF MULTIBLADED HINGELESS ROTORS IN HOVER
No full textThe coupled flap-lag-torsion aeroelastic response and stability of multibladed hingeless rotors in the hovering flight condition are investigated. The vortex lattice method, with a three-dimensional prescribed wake geometry, is used for the prediction of unsteady airloads of multibladed rotors undergoing disturbed dynamic motions. Interblade unsteady wake effects due to vortex-phasing phenomena beneath a rotor are numerically calculated by the phase control of wake vortices shed from each blade. The aeroelastic equations of motion of the rotor blade are formulated using a finite element beam model that has no artificial restrictions on the magnitudes of displacements and rotations due to the degree of nonlinearity. Numerical results of the steady equilibrium deflections and the lead-lag damping and frequency are presented for two-, three-, and four-bladed stiff-inplane rotors, and are compared with those obtained from a two-dimensional quasisteady strip theory with steady and uniform inflow
A Refinement of Sequential Equilibrium.
No full textThe author proposes a refinement of seq uential equilibrium for extensive form games by generalizing a restri ction proposed for signaling games in Cho and D. M. Kreps (1987). The restriction is that beliefs must not assign positive weight to the p ossibilities that can be excluded through reasonable introspection ba sed on the data available as common knowledge. A new technique is dev eloped in order to prove the existence of forward induction equilibri um, which consists of two steps. First, the author establishes the ge neric existence of forward induction equilibrium by exploiting the re sults of E. Kohlberg and J. F. Mertens (1986). Then, he shows that th e forward induction equilibrium correspondence is upper hemicontinuou s in the outcome space with respect to the changes of parameters of t he game. Copyright 1987 by The Econometric Society.
AEROELASTIC STABILITY OF HINGELESS ROTOR BLADE IN HOVER USING LARGE DEFLECTION THEORY
No full textThe coupled flap-lag-torsion aeroelastic stability of a hingeless rotor blade in hover is investigated using finite elements based on large deflection beam theory. The finite element equations of motion for beams arbitrary large displacements and rotations, but small strains, are obtained from Hamilton's principle. The stability boundary is calculated assuming blade motions to be small perturbations about the nonlinear steady equilibrium deflections, which are obtained through an iterative Newton-Raphson method. The p-k modal flutter analysis based on coupled rotating natural modes is used. Various unsteady two-dimensional strip theories am used to evaluate the aerodynamic loads. The sensitivity of the stability boundary to these aerodynamic assumptions is examined. Numerical results of the steady deflections and stability boundaries are presented for some representative blade configurations and also compared with those given in previous moderate deflection type theories
Interview with Donovan Cho
No full textDonovan Steven Cho was born on December 2nd, 1952 at Kona Hospital to Beatrice Madawi and Henry Cho Sr. Donovan grew up with five siblings in a Korean, Japanese, and Hawaiian household along Hōnaunau, helping his parents with their coffee and plumeria flower businesses. Cho would go on to receive his B.A. in Horticulture from UH Mānoa before returning home to Kona to be a commercial fisherman with his brother. Along the coastlines, Cho raised three sons with his wife. He dedicated some years to being an Inspector at Kona airport, but would eventually return to the work he loves most. Cho continues to enjoy fishing off Hōnaunau in his free time, putting into practice the lessons he learned from his father and grandfather
CHO-Bearing Molecules in Comet 67P/Churyumov-Gerasimenko
No full textIn 2004, the Rosetta
spacecraft was sent to comet 67P/Churyumov-Gerasimenko
for the first ever long-term investigation of a comet. After its arrival
in 2014, the spacecraft spent more than 2 years in immediate proximity
to the comet. During these 2 years, the ROSINA Double Focusing Mass
Spectrometer (DFMS) onboard Rosetta discovered a coma with an unexpectedly
complex chemical composition that included many oxygenated molecules.
Determining the exact cometary composition is an essential first step
to understanding of the organic rich chemistry in star forming regions
and protoplanetary disks that are ultimately conserved in cometary
ices. In this study, a joint approach of laboratory calibration and
space data analysis was used to perform a detailed identification
and quantification of CHO compounds in the coma of 67P/Churyumov-Gerasimenko.
The goal was to derive the CHO compound abundances relative to water
for masses up to 100 u. For this study, the May 2015 postequinox period
represents the best bulk abundances of comet 67P/Churyumov-Gerasimenko.
A wide variety of CHO compounds were discovered, and their bulk abundances
were derived. Finally, these results are compared to abundances of
CHO-bearing molecules in other comets, obtained mostly from ground-based
observations and modeling
SUPERSONIC FLUTTER ANALYSIS OF CLAMPED SYMMETRICAL COMPOSITE PANELS USING SHEAR DEFORMABLE FINITE-ELEMENTS
No full textTHE flutter analysis of composite panels in supersonic flow has been performed by the finite element method based on the first-order shear deformable theory. The computational results of the vibration and flutter analysis agree well with the results given in the available references. Flutter boundaries have been obtained for both cross-ply and angle-ply composite plates. Also, the flutter analysis has been performed for both rectangular and trapezoidal plates with clamped edges. The plate aspect ratio, flow direction, and fiber orientation affect greatly the flutter boundaries
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
