166,511 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
CHO-S master cell line generation.
No full text(A) The H11 locus was cleaved, using CRISPR/Cas9, to encourage integration of the landing pad donor. A successful knock-in at the H11 locus generates a master cell line that has two PhiC31 attP sites, and that co-expresses three proteins via a PGK promoter-driven transcript. (B) Genotyping of the 5’-arm and 3’-arm in the CHO-S master cell line. PCR was performed with genomic DNA and specific primer pairs. 1: CHO-S parental cell line with 5’-arm primers; 2: CHO-S master cell line ("4–6") with 5’-arm primers; 3: CHO-S parental cell line with 3’-arm primers; 4: CHO-S master cell line ("4–6") with 3’-arm primers.</p
CHO-K1 sejtek osztódási és letapadási idejének, valamint területének meghatározási módszertana
No full textDolgozatomban a CHO-K1 sejtvonalból származó sejteket vizsgáltam. Szakirodalom kutatást végeztem alapvető tulajdonságaikról, majd az osztódási és letapadási idejüknek, valamint területüknek meghatározási módszertanát tanulmányoztam, amit egy általam tenyésztett CHO-K1 kultúrán keresztül be is mutattam.DOgjBiológiaBSc/B
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Gonioctena (Brachyphytodecta) mauroi Cho & Borowiec 2016
No full textGonioctena (Brachyphytodecta) mauroi Cho & Borowiec, 2016 (Figs 11, 13) Gonioctena (Brachyphytodecta) mauroi Cho & Borowiec, 2016a: 176 (original description). Type locality. ‘ Vietnam, Lang Son Province, Loc Binh, Mt. Mau Son’. Type material examined. HOLOTYPE: ♂, ‘Tonkin, Montes Mauson, April, Mai 2-3000’, H. Fruhstorfer // HOLOTYPUS Gonioctena (Brachy.) mauroi sp. n. Cho & Borowiec 2015’ (LMCM). Description. See CHO & BOROWIEC (2016a). Differential diagnosis. Gonioctena (B.) mauroi is very similar to G. (B.) longshengensis sp. nov. and G. (B.) medvedevi in body shape, coloration and setose aedeagus. From these two species, G. (B.) mauroi can be distinguished only by aedeagus subparallel-sided with apical process widest in middle and slightly narrower than median lobe (subparallel-sided with apical process broad, widest in apical 1/3 and subequal to median lobe in width in G. (B.) longshengensis sp. nov. and moderately narrowed with apical process moderately widened to lateral tooth-like projections near apex in G. (B.) medvedevi). Gonioctena (B.) kuatunensis sp. nov. differs in semicircular apex of aedeagus (triangular or subtriangular in other species). Distribution. Vietnam: Lang Son Province (Fig. 13).Published as part of Cho, Hee-Wook, 2017, Two new species of the Gonioctena mauroi species-group from China (Coleoptera: Chrysomelidae: Chrysomelinae), pp. 173-181 in Acta Entomologica Musei Nationalis Pragae 57 (1) on page 180, DOI: 10.1515/aemnp-2017-0066, http://zenodo.org/record/531615
Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO-8
No full text<p><b>Copyright information:</b></p><p>Taken from "Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO"</p><p>http://www.biomedcentral.com/1471-2210/7/8</p><p>BMC Pharmacology 2007;7():8-8.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1931592.</p><p></p>or 10 min with indicated concentrations of Ac-DNLD-CHO (●), Ac-DEVD-CHO (○), Ac-DQTD-CHO (△), or Ac-DMQD-CHO (□), and then the activities of the caspases were measured with each substrate as described in ''Methods''. The kinetics data presented are the means of three independent experiments
Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO-0
No full text<p><b>Copyright information:</b></p><p>Taken from "Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO"</p><p>http://www.biomedcentral.com/1471-2210/7/8</p><p>BMC Pharmacology 2007;7():8-8.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1931592.</p><p></p>or 10 min with indicated concentrations of Ac-DNLD-CHO (●), Ac-DEVD-CHO (○), Ac-DQTD-CHO (△), or Ac-DMQD-CHO (□), and then the activities of the caspases were measured with each substrate as described in ''Methods''. The kinetics data presented are the means of three independent experiments
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
The Anti-Inflammatory Actions of Lcy-2-Cho, a Carbazole Analogue, in Vascular Smooth Muscle Cells
No full textLCY-2-CHO has anti-infl amm atory actions on macrophages. To understand its therapeutic implication in atherosclerosis, we examined its effects on the expressions of antiinflammatory and inflammatory proteins in cultured rat aortic vascular smooth muscle cells (VSMC). LCY-2-CHO is able to induce heme oxygenase-1 (HO-1) protein expression through a transcriptional action. The HO-1 inducting effect of LCY-2-CHO was inhibited by SB203580, N (G)-nitro-L- arginine methylester (L-NAME), and wortmannin, but was not affected by U0126 or SP600125. In accordance LCY-2 -CHO increased protein phosphorylation of p38, Akt, and eNOS. Nrf 2 is a transcription factor essential for HO-1 gene induction and we showed that LCY-2-CHO is able to cause Nrf2 nuclear translocation and this action depends on p38, Akt and eNOS. in addition to induce anti-inflammatory HO-1 , LCY- 2-CHO reduced interleukin-lp (IL-1 beta)-induced inflammatory mediators, inducible nitric oxide synthase ( iNOS), cyclooxygenase-2 (COX- 2), growth-related oncogene protein-alpha (GRO-alpha), and interleukin-8 ( IL-8). Inhibitory effect on IL-lp-mediated NF-KB activation was evidenced by the diminishment of I kappa B kinase (IKK) phosphorylation and I kappa B alpha degradation. In contrast , IL-1 beta-mediated ERK and JNK activations were not changed by LCY-2-CHO, while p38 activation by IL-1 beta and LCY-2-CHO displayed the nonadditivity. Taken together, given the overall anti-inflammatory properties of LCY-2-CHO in VSMC, in terms to induce HO-1 gene expression and inhibit inflammatory gene expression, these results highlight the therapeutic potential of LCY-2-CHO in atherosclerosis. (C) 2007 Elsevier Inc. All rights reserved
Gonioctena (Brachyphytodecta) medvedevi Cho & Borowiec 2016
No full text<i>Gonioctena</i> (<i>Brachyphytodecta</i>) <i>medvedevi</i> Cho & Borowiec, 2016 <p>(Figs 12–13)</p> <p> <i>Gonioctena</i> (<i>Brachyphytodecta</i>) <i>flavipennis</i> (partim): YANG et al. (2014): 374 (taxonomy, misidentification).</p> <p> <i>Gonioctena</i> (<i>Brachyphytodecta</i>) <i>medvedevi</i> Cho & Borowiec, 2016a: 177 (original description).</p> <p> <b>Type locality.</b> Vietnam, Vinh Phuc Province, Tam Dao.</p> <p> <b>Type material examined.</b> HOLOTYPE: ♂, ‘ Vietnam, Prov.Vinh-phu (divided into Vinh Phuc and Phu Tho), Tam-dao, L. Medvedev @ Dang Dap // on <i>Ormosia</i> (Fab.) // HOLOTYPUS <i>Gonioctena</i> (<i>Brachy</i>.) <i>medvedevi</i> sp. n. Cho & Borowiec 2014’ (LMCM). PARATYPES: 1 ♂ 1 ♀, same data as holotype plus ‘PARATYPUS <i>Gonioctena</i> (<i>Brachy</i>.) <i>medvedevi</i> sp. n. Cho & Borowiec 2014’ (LMCM); 1 ♂, same data as preceding paratype (TLMF); 1 ♂, ‘ Vietnam, TamDao, 900 m, 4.VI.1987, L. Medvedev et al. // PARATYPUS <i>Gonioctena</i> (<i>Brachy</i>.) <i>medvedevi</i> sp. n. Cho & Borowiec 2014’ (LMCM).</p> <p> <b>Description.</b> See CHO & BOROWIEC (2016a).</p> <p> <b>Differential diagnosis.</b> <i>Gonioctena</i> (<i>B</i>.) <i>medvedevi</i> is very similar to <i>G</i>. (<i>B</i>.) <i>longshengensis</i> sp. nov. and <i>G</i>. (<i>B</i>.) <i>mauroi</i> in body shape, coloration and setose aedeagus. From these two species, <i>G</i>. (<i>B</i>.) <i>medvedevi</i> can be distinguished only by aedeagus moderately narrowed with apical process moderately widened to lateral tooth-like projections near apex (subparallelsided with apical process broad, widest in apical 1/3 and subequal to median lobe in width in <i>G</i>. (<i>B</i>.) <i>longshengensis</i> sp. nov. and subparallel-sided with apical process widest in middle and slightly narrower than median lobe in <i>G</i>. (<i>B</i>.) <i>mauroi</i>). <i>Gonioctena</i> (<i>B</i>.) <i>kuatunensis</i> sp. nov. differs in semicircular apex of aedeagus (triangular or subtriangular in other species).</p> <p> <b>Distribution.</b> Vietnam: Vinh Phuc Province (Fig. 13).</p> <p> <b>Remarks.</b> The male genitalia of <i>Gonioctena</i> (<i>B.</i>) <i>flavipennis</i> (Jacoby, 1888) illustrated by YANG et al. (2014) without any doubts refer to <i>G</i>. (<i>B.</i>) <i>medvedevi</i>. The correct drawings of male genitalia of <i>G</i>. (<i>B.</i>) <i>flavipennis</i> were provided by CHO & BOROWIEC (2016a).</p>Published as part of <i>Cho, Hee-Wook, 2017, Two new species of the Gonioctena mauroi species-group from China (Coleoptera: Chrysomelidae: Chrysomelinae), pp. 173-181 in Acta Entomologica Musei Nationalis Pragae 57 (1)</i> on pages 180-181, DOI: 10.1515/aemnp-2017-0066, <a href="http://zenodo.org/record/5316150">http://zenodo.org/record/5316150</a>
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