1,992,287 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
CHO-K1 B2M assay performance on CHO-K1 genomic DNA.
No full textThe CHO-K1 B2M qPCR assay was tested against a fourfold serial dilution of CHO-K1 genomic DNA spanning 0.025–102.4 ng gDNA. Graphing the Ct versus the log of plasmid copies demonstrates a linear range of 0.025–102.4 ng gDNA with R2 values approaching 1 and slope values close to -3.3.</p
CHO-S master cell line generation.
No full text(A) The H11 locus was cleaved, using CRISPR/Cas9, to encourage integration of the landing pad donor. A successful knock-in at the H11 locus generates a master cell line that has two PhiC31 attP sites, and that co-expresses three proteins via a PGK promoter-driven transcript. (B) Genotyping of the 5’-arm and 3’-arm in the CHO-S master cell line. PCR was performed with genomic DNA and specific primer pairs. 1: CHO-S parental cell line with 5’-arm primers; 2: CHO-S master cell line ("4–6") with 5’-arm primers; 3: CHO-S parental cell line with 3’-arm primers; 4: CHO-S master cell line ("4–6") with 3’-arm primers.</p
Interview with Donovan Cho
No full textDonovan Steven Cho was born on December 2nd, 1952 at Kona Hospital to Beatrice Madawi and Henry Cho Sr. Donovan grew up with five siblings in a Korean, Japanese, and Hawaiian household along Hōnaunau, helping his parents with their coffee and plumeria flower businesses. Cho would go on to receive his B.A. in Horticulture from UH Mānoa before returning home to Kona to be a commercial fisherman with his brother. Along the coastlines, Cho raised three sons with his wife. He dedicated some years to being an Inspector at Kona airport, but would eventually return to the work he loves most. Cho continues to enjoy fishing off Hōnaunau in his free time, putting into practice the lessons he learned from his father and grandfather
CHO-K1 sejtek osztódási és letapadási idejének, valamint területének meghatározási módszertana
No full textDolgozatomban a CHO-K1 sejtvonalból származó sejteket vizsgáltam. Szakirodalom kutatást végeztem alapvető tulajdonságaikról, majd az osztódási és letapadási idejüknek, valamint területüknek meghatározási módszertanát tanulmányoztam, amit egy általam tenyésztett CHO-K1 kultúrán keresztül be is mutattam.DOgjBiológiaBSc/B
Cacopsylla baccatae Cho & Burckhardt 2017
No full textCacopsylla baccatae Cho & Burckhardt in Cho et al., 2017 (Figs. 73–74) Cacopsylla baccatae Cho & Burckhardt in Cho et al., 2017a: 539. Cacopsylla (Hepatopsylla) baccatae; Kwon & Kwon (2020: 141). Distribution in Korea. GB, GW (Cho et al. 2017a; Kwon & Kwon 2020, as Cacopsylla (Hepatopsylla) baccatae) (NHMB, SNU). Host plant. Malus baccata (L.) Borkh. (Rosaceae) (Cho et al. 2017a).Published as part of Cho, Geonho, Burckhardt, Daniel & Lee, Seunghwan, 2022, Check list of jumping plant-lice (Hemiptera: Psylloidea) of the Korean Peninsula, pp. 1-91 in Zootaxa 5177 (1) on page 39, DOI: 10.11646/zootaxa.5177.1.1, http://zenodo.org/record/702193
CHO-Bearing Molecules in Comet 67P/Churyumov-Gerasimenko
No full textIn 2004, the Rosetta
spacecraft was sent to comet 67P/Churyumov-Gerasimenko
for the first ever long-term investigation of a comet. After its arrival
in 2014, the spacecraft spent more than 2 years in immediate proximity
to the comet. During these 2 years, the ROSINA Double Focusing Mass
Spectrometer (DFMS) onboard Rosetta discovered a coma with an unexpectedly
complex chemical composition that included many oxygenated molecules.
Determining the exact cometary composition is an essential first step
to understanding of the organic rich chemistry in star forming regions
and protoplanetary disks that are ultimately conserved in cometary
ices. In this study, a joint approach of laboratory calibration and
space data analysis was used to perform a detailed identification
and quantification of CHO compounds in the coma of 67P/Churyumov-Gerasimenko.
The goal was to derive the CHO compound abundances relative to water
for masses up to 100 u. For this study, the May 2015 postequinox period
represents the best bulk abundances of comet 67P/Churyumov-Gerasimenko.
A wide variety of CHO compounds were discovered, and their bulk abundances
were derived. Finally, these results are compared to abundances of
CHO-bearing molecules in other comets, obtained mostly from ground-based
observations and modeling
Erratum: 3D bioprinted in vitro secondary hyperoxaluria model by mimicking intestinal-oxalatemalabsorption-related kidney stone disease (Applied Physics Reviews (2022) 9 (041408) DOI: 10.1063/5.0087345)
No full text© 2023 Author(s).This article was originally published online on 21 November 2022 with an incorrect affiliation identifier for author Dong-Woo Cho. It is correct as it appears above. All online versions of this article were corrected on 23 November 2022. AIP Publishing apologizes for this error.11Nsciescopu
Cyamophila floribundae Cho & Burckhardt 2017
No full textCyamophila floribundae Cho & Burckhardt, 2017 (Fig. 135) Cyamophila floribundae Cho & Burckhardt, 2017a: 551. Distribution in Korea. JJ (Cho et al. 2017a) (NHMB, NIBR, SNU). Host plant. Maackia floribunda (Miq.) Takeda (Fabaceae) (Cho et al. 2017a).Published as part of Cho, Geonho, Burckhardt, Daniel & Lee, Seunghwan, 2022, Check list of jumping plant-lice (Hemiptera: Psylloidea) of the Korean Peninsula, pp. 1-91 in Zootaxa 5177 (1) on page 60, DOI: 10.11646/zootaxa.5177.1.1, http://zenodo.org/record/702193
The Anti-Inflammatory Actions of Lcy-2-Cho, a Carbazole Analogue, in Vascular Smooth Muscle Cells
No full textLCY-2-CHO has anti-infl amm atory actions on macrophages. To understand its therapeutic implication in atherosclerosis, we examined its effects on the expressions of antiinflammatory and inflammatory proteins in cultured rat aortic vascular smooth muscle cells (VSMC). LCY-2-CHO is able to induce heme oxygenase-1 (HO-1) protein expression through a transcriptional action. The HO-1 inducting effect of LCY-2-CHO was inhibited by SB203580, N (G)-nitro-L- arginine methylester (L-NAME), and wortmannin, but was not affected by U0126 or SP600125. In accordance LCY-2 -CHO increased protein phosphorylation of p38, Akt, and eNOS. Nrf 2 is a transcription factor essential for HO-1 gene induction and we showed that LCY-2-CHO is able to cause Nrf2 nuclear translocation and this action depends on p38, Akt and eNOS. in addition to induce anti-inflammatory HO-1 , LCY- 2-CHO reduced interleukin-lp (IL-1 beta)-induced inflammatory mediators, inducible nitric oxide synthase ( iNOS), cyclooxygenase-2 (COX- 2), growth-related oncogene protein-alpha (GRO-alpha), and interleukin-8 ( IL-8). Inhibitory effect on IL-lp-mediated NF-KB activation was evidenced by the diminishment of I kappa B kinase (IKK) phosphorylation and I kappa B alpha degradation. In contrast , IL-1 beta-mediated ERK and JNK activations were not changed by LCY-2-CHO, while p38 activation by IL-1 beta and LCY-2-CHO displayed the nonadditivity. Taken together, given the overall anti-inflammatory properties of LCY-2-CHO in VSMC, in terms to induce HO-1 gene expression and inhibit inflammatory gene expression, these results highlight the therapeutic potential of LCY-2-CHO in atherosclerosis. (C) 2007 Elsevier Inc. All rights reserved
- …
