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Buckypaper (BP) as absorbable adhesive for surgical application in abdominal wall issues
No full textBy: Sun, P.; Hu, S. B.; Cheng, X.; Li, M.; Guo, B.; Song, Z. F.; Zhang, Y.; Zheng, Q. C.; Hoyuela, C.; Vega, L.; Carvajal, F.; Blay, L.; Juvany, M.; Camps, J.; Veres, A.; Besora, P.; Trias, M.; Feliu, X.; Bellanova, G.; Viel, G.; et al. In: Hernia. April 2015 19(1):S157-S165; Springer Paris Language: English, Database: Springer Nature Journals
PROTPCOLLO DI RICERCA PUBBLICATO SU: ClinicalTrials.gov Identifier: NCT02137018 https://register.clinicaltrials.gov FINANZIAMENTO RICERCA DI ATENEO INTERDIPARTIMENTALE prot. C26A10HCNR, founding of 35000€ DIPARTIMENTO DI CHIMICA: Prof Lucio D'Ilario, Prof. Andrea Martinelli, DIPARTIMENTO DI CHIRURGIA "PARIDE STEFANINI": Dott. Massimo Chiaretti , prot letter 0003306 project n° 304P25, interfaculty Chemistry Dep, General Surgery Paride Stefanini Dep. http://www.uniroma1.it Location National Health Institute (ISS Istituto Superiore di Sanità), Viale Regina Elena 299 Rome, Roma, Italy, 00161 "BP as a New Device for Surgery and Solid Cancer and Hematopoietic System Tumors Treatment. Effects of BP Implantation" (DM159) ClinicalTrials.gov Protocol and Results Registration System (PRS) Receipt Release Date: 12/30/2014 The objective of this project is to obtain results that can direct the search for the ultimate realization of a prosthetic device for use in abdominal surgery. Will be conducted systematic experiments in 30 New Zealand female rabbits (R1-R30), weighting about 3000 g (Harlan Laboratories).Campanelli, Giampiero. Hernia Volume: 19 Issue S1 (2015) ISSN: 1265-4906 Online ISSN: 1248-9204
Buckypaper (BP) as absorbable adhesive for surgical application in abdominal wall issues
Chiaretti M(1), Martinelli A(2), Carru GA(3), Alunni Fegatelli D(4), Chiaretti AM(5), Consentino P(6), Chiaretti AI(1), Procacciante F(1)
(1) Department of General Surgery “Paride Stefanini”, Sapienza University, Viale del Policlinico 155, Rome, 00161 (Italy)
(2) Department of Chemistry, Sapienza University, P.le Aldo Moro 5, Rome, 00185 (Italy)
(3) Policlinico Umberto I Hospital, Sapienza University, Viale del Policlinico 155, 00161 - Rome (Italy)
(4) Public Health and Infective Illness, Sapienza University, P.le Aldo Moro 5, Rome, 00185 (Italy)
(5) Istitute of Genetic Risearch G. Salvatore, Scienze e Tecnologie Genetiche Biogem;
(6) Appialab Srl V. Latina, 286 00179 Rome (+39) 067825111www.appialab.it
E-mail: [email protected]
Introduction: One of the key issues relating to abdominal prosthetic surgery is the fixation of the abdominal mesh. Mesh displacement, improper or blind application of metal tacks and staples in laparoscopic surgery are the main causes of complications such as seroma[1], postoperative pain[2] and recurrence of hernia[3]. The well-established use of fibrin glue[4], or self-gripping commercial meshes, or suture meshes to fix prosthesis to damaged tissue may represent a risk associated to the transmission of unknown diseases related to the use of blood-derived materials[4] or pain caused by nerve entrapment or displacement. Moreover, it has been observed[1,5] that the use of fibrin glue for mesh fixation may be associated with incidence of postoperative seroma. An effective alternative to the conventional prosthesis fixation methods may be represented by devices able to adhere strongly and quickly[1,5] on wet[6] biological tissue. In view of a possible application of BP as adhesive absorbable tape in surgery, preliminary in vivo experiments were carried out on large-size pigs. Methods: We investigated the potential applicability of BP, a self-standing felt composed of multi-walled carbon nanotubes, as an adhesive tape on biological tissue compared with traditional methods of fixation of prosthetic meshes. Based on biological materials from experimentation on rabbits (in progress[7]) we carried out a comparative study on two sets of meshes: one of the sets was fixed with BP and the other set was fixed with traditional methods. We did this by carrying out bench surgery and performing mechanical peeling and shear adhesiveness tests on each set[5,8,9,10]. The in vivo experimentation was carried out on 30 Landrace female pigs weighing about 15 kg initially and 60 kg when they were sacrificed. The subjects were divided into two sets i.e., 15 for laparoscopic procedure and 15 for laparotomic procedure. Each group was further subdivided into an experimental set of 11 subjects for mesh fixation with BP and a control set of 4 subjects for traditional mesh fixation. Results: Experimental and control subsets did not show mortality or morbidity. Even after 90 days from the operation no significant neurovegetative or behavioral differences were observed. The BP fixing method shows stronger adhesiveness on wet tissues[6] than the mentioned traditional fixing methods. Checks on body weight monitoring curves show no significant differences between BP and control groups. Necroscopical and histological investigations show that BP elicits minimal adverse tissue response. Nanometric carbon nanotube aggregates were phagocytized by macrophages. Further, BP fragments were also observed in Bowman's urinary space. Finally, around the BP, a capsule of loose fibrous tissue is formed by collagen fibers deposited by fibroblasts which indicate a moderate cicatrisation reaction. Conclusion: Findings from our experiment are compatible with a stronger adhesiveness on wet biological tissue for the BP fixing method compared to traditional methods. Further studies are required to fully assess the nature of the BP debris, their toxicity potential and their metabolization.
References
1. B. P. Jacob, N. J. Hogle, E. Durak, T. Kim, D. L. Fowler, Surg. Endosc. 2007, 21, 629
2. J. R. Eriksen, J. I. Bech, D. Linnemann, J. Rosemberg, Hernia 2008, 12, 483
3. W. B. Gaertner, M. E. Bonsack, J. P. Delaney, Hernia 2010, 14, 375
4. S. Olmi, A. Addis, C. Domeneghini, A. Scaini, E. Croce, Hernia. 2007, 11, 211
5. S. Bellucci, M. Chiaretti, P. Onorato, F. Rossella, M. S. Grandi, P. Galinetto, I. Sacco, F. Micciulla, Nanomedicine 2010, 5, 209
6. A. Martinelli, GA. Carru, L. D'Ilario, F. Caprioli, M. Chiaretti, F. Crisante, I. Francolini, and A. Piozzi. ACS Appl. Mater. Interfaces, DOI:10.1021/am400543s Publication Date (Web): 18 Apr 2013, http://pubs.acs.org on April 19, 2013
7. M. Chiaretti, G. A. Carru, Danilo Alunni Fegatelli, A. M. Chiaretti, A. Martinelli, (in progress)
8. M. Chiaretti, G. Mazzanti, S. Bosco, S. Bellucci, A. Cucina, F. Le Foche, G. A. Carru, S. Mastrangelo, A. Di Sotto, R. Masciangelo, A. M. Chiaretti, C. Balasubramanian, G. De Bellis, F. Micciulla, N. Porta, G. Deriu, A. Tiberia, J. Phys.: Condens. Matter 2008,20, 474203
9. A. Di Sotto, M. Chiaretti, G. A. Carru, S. Bellucci, G. Mazzanti, Toxicol. Lett. 2009, 184, 192
10. S. Bellucci, M. Chiaretti, A. Cucina, G.A. Carru,A.I. Chiaretti, Nanomedicine 2009, 4, 53
PRP nel trattamento del piede diabetico e dell’osteomielite fistolizzata. Revisione della letteratura e nostra esperienza
No full textLa nostra esperienza evidenzia l’utilità del plasma ricco di piastrine
(PRP) nel trattamento ambulatoriale delle piaghe torpide della pianta
del piede e malleolari in paziente diabetico e/o vasculopatico che
normalmente richiedono lunghi periodi di medicazioni ambulatoriali
complesse. La metodica personale prevede il curettage dopo disinfezione
e debridement della piaga, l’inoculazione di 1-4 UI di insulina pronta
nel cratere ulcerativo, anche nel non diabetico, e se la lesione è sterile,
quindi l’applicazione di PRP, attivato su un supporto di acido ialuronico
per ottenere un panno morbido, elastico e gelatinoso che ne impedisce
la dispersione. Il GP stimola la moltiplicazione fibroblastica che deposita
nuova matrice connettivale. Nel caso di paziente affetto da ulcerazione
da osteomielite e nei casi di infezione della piaga è indispensabile
procedere prima alla sterilizzazione con terapia antibiotica parenterale
mirata. L’approccio multidisciplinare tra chirurgo, immunotrasfusionista,
infettivologo, dermatologo, ortopedico, diabetologo e cardiologo, aiuta a
risolve la complessa problematica. È importane lo stretto coordinamento
tra ambulatorio chirurgico e Centro Trasfusionale per coordinare il
prelievo autologo, la preparazione delle aliquote e l’esecuzione della
medicazione evitando lunghe attese al paziente, già provato dalle proprie
malattie. Anche se vi sono dei rischi intrinseci all’uso dell’attivatore, l’uso
del PRP riduce i tempi di guarigione a 6-8 settimane, velocizza l’esecuzione
della medicazione, abbatte la spesa sanitaria e diventa quindi conveniente
rispetto al trattamento tradizionale.Our experience highlights the usefulness of the platelet-rich plasma (PRP)
in the outpatient treatment of torpid wounds of the foot in diabetic patients
and malleolar and/or vascular disease that usually requires long periods
of outpatient complex dressings. Our method provides disinfection
and debridement of the wound, inoculation of 1-4 IU of rapid insulin
in ulcerative crater, even in non-diabetic, and if the lesion is sterile, the
application of activated PRP on hyaluronic acid support obtaining a soft,
elastic and gelatinous cloth. The GP stimulates fibroblast proliferation
and new connective depositing. In the case of a patient suffering from
osteomyelitis and ulceration in cases of infection of the wound is essential
to proceed priory to sterilization with parenteral antibiotic therapy
targeted. The multidisciplinary approach helps to resolve the complex
issues. The important matter is the close coordination between surgery
and transfusion center to coordinate PRP preparation and execution of
the medication, avoiding long waits. Although there are risks inherent
in the use of the activator, the use of PRP reduces the healing time up to
6-8 weeks, speeds up the execution of the medication, lowers health care
costs, and thus becomes cheaper than the traditional treatment
PRP (Plasma Ricco di Piastrine): trattamento delle piaghe da piede diabetico, dell'osteomielite fistolizzata postraumatica, nostre esperianze in 63 casi clinici, revisione della letteratura.
No full textPRP (PLASMA RICCO DI PIASTRINE): TRATTAMENTO DELLE PIAGHE DA PIEDE DIABETICO, DELL’OSTEOMIELITE FISTOLIZZATA POSTRAUMATICA, NOSTRE ESPERIENZE IN 63 CASI CLINICI, REVISIONE DELLA LETTERATURA.
Massimo Chiaretti*, Giovanna Angela Carru^, Annalisa Italia Chiaretti°, Giancarlo Ceccarelli^, Eleonora Valente§, Alessandra Maria Chiaretti#,
(*)Dipartimento di Chirurgia Generale, Specialità Chirurgiche e Trapianti d’Organo “Paride Stefanini” Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”;
(^)Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”;
(°)Azienda Ospedaliera S. Andrea, II Facoltà di Medicina e Psicologia, Università degli Studi di Roma “La Sapienza”;
(#)Istituto Ricerche Genetiche G. Salvatore, Consorzio Interuniversitario di Scienze e Tecnologie Genetiche “Biogem”;
(§)Policlinico Agostino Gemelli, Università Cattolica del Sacro Cuore, Roma
Introduzione: la nostra esperienza evidenzia l’utilità del PRP (Plasma Ricco di Piastrine) nel trattamento ambulatoriale delle piaghe torpide della pianta del piede, del malleolo e della gamba, in pazienti vasculopatici con ulcere torpide e/o diabetici. Tali lesioni richiedono, solitamente, periodi lunghissimi di medicazioni complesse, spesso senza raggiungere il risultato atteso. Materiali e Metodi: la metodica personale prevede il debridement dopo disinfezione della piaga, l’inoculazione di 1-4 UI di insulina pronta nel cratere ulcerativo, l’applicazione di circa 5 ml di PRP su spugna di acido ialuronico (Hyalofill-F Fidia® Italia che serve ad impedirne la dispersione della parte liquida o non completamente gelificata), attivata con Plateltex®. L’applicazione si esegue facendo gocciolare il PRP sulla spugna di acido ialuronico, sulla quale si fa poi gocciolare l’attivatore (bicomponente: calcio gluconato e batroxobina, 5 BU, Batroxobin Units). Gli ioni calcio (Ca++) del calcio gluconato saturano l’anticoagulante presente nel PRP, preparato dal nostro Centro Trasfusionale (CT) da sangue autologo, suddiviso in aliquote congelate. La batroxobina serve, invece, a trasformare il fibrinogeno in fibrina, in combinazione con gli ioni Ca++ del calcio gluconato, formando il reticolo gelificato. La gelatina neoformata si presenta come un panno morbido elastico-gelatinoso, applicabile nella piaga per riempire la cavità da trattare. Il PRP è ricco dei fattori di crescita (PDGF, TGF, EGF) che servono a stimolare la moltiplicazione dei fibroblasti che aumentando di numero, promuovono la fioritura dei bottoni di granulazione, depositano una quantità consistente di matrice connettivale, trasformando la piaga torpida in una ferita in cicatrizzazione per seconda intenzione. Nel caso di piaga infetta o paziente con fistola osteomielitica è indispensabile sterilizzare la lesione infetta, prima di procedere al trattamento con PRP. Tale procedura prevede esami colturali seriati con antibioticoterapia parenterale mirata. Discussione: Il metodo prevede l’esecuzione in camera operatoria sterile ambulatoriale. L’approccio multidisciplinare è indispensabile e prevede la costituzione di un PAC (Pacchetto Assistenziale Complesso) che coinvolga Chirurgo Generale e Vascolare, Diabetologo, Infettivologo, Immunotrasfusionista, Dermatologo, Ortopedico, Cardiologo e Specialista per l’esecuzione di Ecocolordoppler arterioso e venoso. Conclusioni: l’approccio multidisciplinare e lo stretto coordinamento tra ambulatorio chirurgico e CT evitano inutili attese al Paziente, accorciano i tempi di esecuzione della medicazione, abbattono i costi per l’impiego di materiali, rendendo il trattamento con PRP conveniente rispetto a quello tradizionale. Per aumentare la casistica ed ottenere i numeri necessari ad una validazione statistica è in corso la realizzazione di un protocollo multicentrico.
Bibliografia-Sitografia
1) Butalia S, Palda VA, Sargeant RJ, Detsky AS, Mourad O. Does this patient with diabetes have osteomyelitis of the lower extremity? JAMA. 2008 Feb 20;299(7):806-13. Review. PubMed PMID: 18285592.
2) Dinh MT, Abad CL, Safdar N. Diagnostic accuracy of the physical examination and imaging tests for osteomyelitis underlying diabetic foot ulcers: meta-analysis. Clin Infect Dis. 2008 Aug 15;47(4):519-27. PubMed PMID: 18611152.
3) Joseph WS, Lipsky BA. Medical therapy of diabetic foot infections. J Vasc Surg. 2010 Sep;52(3 Suppl):67S-71S. Review. PubMed PMID: 20804935.
4) Powlson AS, Coll AP. The treatment of diabetic foot infections. J Antimicrob Chemother. 2010 Nov;65 Suppl 3:iii3-9. Review. PubMed PMID: 20876626.
5) Palombi M, Fratto D: Trattamento del piede diabet. con medicazioni avanzate: Atti SICG, Rome Sept.14-16/2006
6) Pastore P, Piermattei A: Trattamento delle ulcere croniche degli arti inferiori con V.A.C.® (vacum assisted closure) therapy e sostituto dermico a base di acido ialuronico (Hyalomatrix®); Atti SICG, Rome Sept.14-16/2006
7) Fattore di crescita piastrinico per le ulcere diabetiche. The Medical Letter, 1998; 27: 18;
8) Joshi N: Infections in Patients with Diabetes Mellitus. N Engl J Med 1999; 341: 1906-12;
9) Bauer E: Foot Ulcers. N. Engl J Med 2000; 343: 787-93;
10) Fattore di crescita piastrinico per le ulcere diabetiche. The Medical Letter, 1998; 27: 18;
11) Joshi N: Infections in Patients with Diabetes Mellitus. N Engl J Med 1999; 341: 1906-12;
12) Adam J: Cutaneous wound healing. N. Engl. J Med 1999; 341: 738-46;
13) Slater M: Involvement of platelets in stimulating Osteogenic Activity. J orthop Res. 1995; 13: 5;
14) Dean H: Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 1997; 55: 1294-99;
15) Dean H: A technique for improving the handling of particulate cancellous bone and marrow grafts using platelets gel. J Oral Maxillofac. Surg 1998; 56: 1217-18;
16) Gehring S: Preparation of autologous platelets for the oftalmo1ogic treatrnnt of macular holes. Transfusion, February 1999; 39: 144-48;
17) Zimmermann R: Different preparation methods to obtain platelet component as a source of growth factors for local application. Transfusion 2001; 41:1217-24;
18) Colaprisco G, Borean A: Il Gel piastrinico: come e quando. Progressi in Emaferesi 2002; 7: 39-51.
19) Dalla Paola L., Cogo A: Use of hyaluronic acid derivatives and cultured autologous fibroblasts and keratinocytes in an estensive wound of the lower limb in a diabetic patient, case report. Ostomy Wound Management 2002; 48:46.
20) Blume P.A., Paragas L.K.: Single-stage surgical treatment of noninfected diabetic foot ulcers. Plast.Reconstr.Surg. 2002; 109:601.
21) Bonadeo P., Marazzi M: Wound Bed Preparation: evoluzione della pratica clinica secondo i principi del T.I.M.E., 2004, Aretrè, Milano.
22) Campoccia D., Doherty P: Semisynthetic resorbable materials from hyaluronan esterification. Biomaterials, 1998, 19:2101-2127.
23) Zhon L.H., Vahm W.K: Slow release iodin preparation and wound healing: in vitro effects consistent with lack off in vivo toxicity in human chronic wounds. Br. J. Clin. Derm., 2001; 146 : 365-374.
24) Tognazzo S, Gemmati D: Varianti geniche del fattore XIII per la prognosi delle ulcere venose non tendenti alla guarigione. Attuali applicazioni e prospettive cliniche.
25) massimochiaretti.wordpress.com
26) Il gel piastrinico nel trattamento del “piede diabetico”: esperienza preliminare, Chiaretti M., Ferrazza G., Fracassi R., Negro A., Tuscano D. 2007;31(1):259-260; ISSN:0391-3619
27) The Effect of Autologous Activated Platelet Rich Plasma (AA-PRP) Injection on Pattern Hair Loss: Clinical and Histomorphometric Evaluation.Cervelli V, Garcovich S, Bielli A, Cervelli G, Curcio BC, Scioli MG, Orlandi A, Gentile P. Biomed Res Int. 2014;2014:760709. doi: 10.1155/2014/760709. Epub 2014 May 6.
28) High Insulin Level-Induced Down-Regulation of Erk-1/IGF-1R/FGFR-1 Signaling Is Required for Oxidative Stress-Mediated Apoptosis of Adipose Tissue-Derived Stem Cells. Scioli MG, Cervelli V, Arcuri G, Gentile P, Doldo E, Bielli A, Bonanno E, Orlandi A. J Cell Physiol. 2014 May 13. doi: 10.1002/jcp.24667. [Epub ahead of print]
29) The biomolecular basis of adipogenic differentiation of adipose-derived stem cells. Scioli MG, Bielli A, Gentile P, Mazzaglia D, Cervelli V, Orlandi A. Int J Mol Sci. 2014 Apr 16;15(4):6517-26. doi: 10.3390/ijms15046517.
30) Adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical evaluation for cell-based therapies in patients with scars on the face. Gentile P, De Angelis B, Pasin M, Cervelli G, Curcio CB, Floris M, Di Pasquali C, Bocchini I, Balzani A, Nicoli F, Insalaco C, Tati E, Lucarini L, Palla L, Pascali M, De Logu P, Di Segni C, Bottini DJ, Cervelli V. J Craniofac Surg. 2014 Jan;25(1):267-72. doi: 10.1097/01.scs.0000436746.21031.ba.
31) Gel Piastrinico: Trattamento del Piede Diabetico e dell’osteomielite fistolizzata. Revisione della letteratura e Nostra Esperienza in 61 Casi Clinici. Massimo Chiaretti*, Giancarlo Ferrazza+, Giovanna Angela Carru^, Gianna Barrella+ , Giancarlo Ceccarelli^, Pietro Bondani*, Giulia Diamantini *, Annalisa Italia Chiaretti°, Fabio Procacciante* SIC 2012 Roma, Parco della Musica
32) Gel Piastrinico: Trattamento del Piede Diabetico e dell’osteomielite fistolizzata. Revisione della letteratura e Nostra Esperienza in 61 Casi Clinici. Massimo Chiaretti*, Giovanna Angela Carru^, Giancarlo Ceccarelli^, Giulia Diamantini*, Annalisa Italia Chiaretti°, Fabio Procacciante* XXXV Congresso Nazionale Società Italiana Medicina Estetica SIME 2014 Roma, Centro Congressi Cavalieri Waldorf Astoria.compact disc ISSN 2038-331
Buckypaper (bp) as absorbable adhesive for surgical application in abdominal wall defects
No full textTOPICS: Biotecnologia ed innovazioni tecnologiche. Le proposte italiane nella ricerca.
BUCKYPAPER (BP) AS ABSORBABLE ADHESIVE FOR SURGICAL APPLICATION IN ABDOMINAL WALL DEFECTS
Massimo Chiaretti(1), Andrea Martinelli(2), Giovanna Angela Carru(3), Emanuela D’Amore(4), Alessandra Maria Chiaretti(5), Fabio Faiola(6), Paola Consentino(7), Eleonora Valente(8), Annalisa Italia Chiaretti(1)
(1)Department of General Surgery “Paride Stefanini”, Sapienza University of Rome, Viale del Policlinico 155, Rome, 00161 (Italy)
(2)Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, Rome, 00185 (Italy)
(3)Policlinico Umberto I Hospital, Sapienza University of Rome, Viale del Policlinico 155, 00161 - Rome (Italy)
(4)National Health Institute, Viale Regina Elena 299, 00161 - Rome (Italy)
(5) Istitute of Genetic Risearch G. Salvatore, “Consorzio Interuniversitario di Scienze e Tecnologie Genetiche Biogem”;
(6)Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, Rome, 00161 (Italy)
(7)Appialab Srl V. Latina, 286 00179 Rome (+39) 067825111www.appialab.it
(8)Policlinico Agostino Gemelli, Università Cattolica del Sacro Cuore, Roma
E-mail: [email protected]
Keywords: buckypaper, BP, MWCNTs, medical application, abdominal wall defects surgery, wet adhesion, absorbable adhesive device.
Introduction: One of the main problems related to the abdominal prosthetic surgery is the mesh fixation. Mesh displacement, improper and blind application of metal tacks and staples in laparoscopy surgery are the main causes of complications such as seroma, postoperative pain and recurrence[6-9]. The use of fibrin glue between the prosthesis and the damaged tissue, although it has become a well-established surgical procedure, represents a possible risk associated to the transmission of unknown diseases related to the use of human blood-derived materials [9]. Moreover, it was observed that an increased incidence of postoperative seroma is associated to the use of fibrin glue for mesh fixation[10]. Surfaces able to adhere promptly and strongly on wet biological tissue may represent an effective alternative to the conventional prosthesis fixation methods. This study realized on animal model proposes the application of the nature inspired micro- or nano-patterned adhesive surfaces, by exploiting the scaling effect, according to which the adhesion strength can be enhanced through the reduction of interface adhesive contact size[12]. We experimented evidences on the potential applicability on biological tissue of the BP as an adhesive tape, a self-standing felt composed of entangled multi-walled carbon nanotubes. In vitro bench surgery, mechanical peeling, and shear adhesion tests, and In vivo tests were tested with prosthetic meshes[2,3,4,10]. BP shows a stronger adhesion, but only on wet tissues[12] much better than both self-gripping commercial mesh and fibrin-glue non-gripping meshes and fabrics. Prompted by these results, we implanted BP in pigs, to assess BP effectiveness as adhesive absorbable prosthetic device and its biocompatibility. After 90 days from the operation we observed no pig behavioral alteration. BP samples preserved their position in the implantation site and mechanical adhesion was enhanced, both in laparoscopy and laparotomic procedure. Materials and Methods. Operated 30 Lantrace female 40-50 Kg pigs, 15 in laparoscopic procedure and 15 in laparotomic procedure. Operated and control subjects did not show mortality or morbidity, no significant neurovegetative or behavioral differences. Results and Discussion. Body weight monitoring graph do not showed any significant difference between BP and control group. The Hematoxilin and Eeosin stained implant section reported BP surface fragmentation that shows the formation of a capsule of loose fibrous tissue, consisting of fibroblasts and collagen fibers, indicative of a weak inflammation reaction. The necroscopy examination showed that the implanted BP favors a cicatrisation process, the mesh appearing wrapped in the inflammatory reaction. Conclusions: a strong BP adhesion on wet biological tissue was measured. In view of a possible application as adhesive absorbable tape in surgery, preliminary in vivo experiments were carried out on big pig model. Necroscopical and histological investigations enlighten that as 90 days after the implantation the BP elicits minimal adverse tissue response. Nanometric carbon nanotube aggregates were phagocytised by macrophages and observed in Bowman's urinary space. The assessment of the BP debris, possible toxicity or confinement or metabolism and accumulation or excretion mechanism needs further studies.
References
1. L. Liu, W. Ma, Z. Zhang,Small 2011, 7, 1504
2. M. Chiaretti, G. Mazzanti, S. Bosco, S. Bellucci, A. Cucina, F. Le Foche, G. A. Carru, S. Mastrangelo, A. Di Sotto, R. Masciangelo, A. M. Chiaretti, C. Balasubramanian, G. De Bellis, F. Micciulla, N. Porta, G. Deriu, A. Tiberia, J. Phys.: Condens. Matter 2008,20, 474203
3. A. Di Sotto, M. Chiaretti, G. A. Carru, S. Bellucci, G. Mazzanti, Toxicol. Lett. 2009, 184, 192
4. S. Bellucci, M. Chiaretti, A. Cucina, G.A. Carru,A.I. Chiaretti, Nanomedicine 2009, 4, 531
5. a) The mean equivalent radius was evaluated by wicking tests carried out in water, assuming a water contact angle of about 80°; b) G. Callegari, I. Tyomkin, K. G. Kornev, A.V. Neimark, Y-L. Hsieh, J. Colloid Interface Sci.2011, 353, 290
6. B. P. Jacob, N. J. Hogle, E. Durak, T. Kim, D. L. Fowler, Surg. Endosc. 2007, 21, 629
7. J. R. Eriksen, J. I. Bech, D. Linnemann, J. Rosemberg, Hernia 2008, 12, 483
8. W. B. Gaertner, M. E. Bonsack, J. P. Delaney, Hernia 2010, 14, 375
9. S. Olmi, A. Addis, C. Domeneghini, A. Scaini, E. Croce, Hernia. 2007, 11, 211
10. S. Bellucci, M. Chiaretti, P. Onorato,F. Rossella, M. S. Grandi, P. Galinetto, I. Sacco,F. Micciulla, Nanomedicine 2010, 5, 209
11. massimochiaretti.wordpress.com
12. A. Martinelli, GA. Carru, L.D'Ilario, F. Caprioli, M. Chiaretti, F. Crisante, I. Francolini, and A. Piozzi. ACS Appl. Mater. Interfaces, DOI:10.1021/am400543s • Publication Date (Web): 18 Apr 2013, http://pubs.acs.org on April 19, 201
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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