1,721,064 research outputs found
Improvement of Citrus clementina Hort. ex Tan. microspore-derived embryoid induction and regeneration
No full textAn improvement of the protocol for haploid induction through anther culture of Citrus clementina Hort. ex Tan. cv. Nules was achieved following the evaluation of a number of the factors affecting androgenesis. The influence of thidiazuron (TDZ) and three temperature pre-treatments (4°C, 25°C, 32°C) on the floral buds with respect to anther culture of C. clementina Hort. ex Tan., cv. Nules was investigated. An increased embryoid production was induced in the medium supplemented with TDZ. Pre-treatment temperatures of 4°C and 25°C were more favorable for embryo production than 32°C. Regeneration of androgenic haploid plantlets from cv. SRA 63 of C. clementina is reported here for the first time
In vitro germination and seedling development of caper (Capparis spinosa L.) mature seeds
No full textCapparis spinosa L. (Capparidaceae) is a perennial tropical and subtropical shrub plant known worldwide. Despite the increasing demand and economic importance of capers, little information is available regarding the propagation of this shrub. In fact, it is usually propagated by seed, but its percentage of germination is very low. In this report, in vitro seed germination and seedling development of Capparis spinosa L. were studied and several treatments were evaluated to determine the ability of several factors to increase the percentage of germination. High variability was observed among the germination percentages of the different treatments (3-81%) due to their different efficiency to break dormancy. Scarification through seed coat rupture has been determinant to improve germination percentage. In vitro obtained seedlings were valuable to establish a micropropagation protocol for caper
In vitro establishment and culture of two Sicilian cultivars of Olea europaea L
No full textSicily is among the Italian regions with the largest cultivation area of olive (Olea europaea L.). The applications of in vitro tissue culture, as practical tool of propagation and germplasm storage, were not sufficiently exploited in this species, because of the lack of efficient in vitro establishment protocols and culture for the majority of the cultivars. In fact, it is well known that the success of in vitro culture is strongly dependent on the species and, within the species, on the cultivars. This study reports the in vitro establishment of two Sicilian olive cultivars, grown in greenhouse. Both genotypes showed a percentage of in vitro sprouting higher than that one previously obtained with explants collected from mature plants grown in open field. The collection time was not determinant on in vitro response
In vitro establishment and culture of two Sicilian cultivars of Olea europaea L
No full textSicily is among the Italian regions with the largest cultivation area of olive (Olea europaea L.). The applications of in vitro tissue culture, as practical tool of propagation and germplasm storage, were not sufficiently exploited in this species, because of the lack of efficient in vitro establishment protocols and culture for the majority of the cultivars. In fact, it is well known that the success of in vitro culture is strongly dependent on the species and, within the species, on the cultivars. This study reports the in vitro establishment of two Sicilian olive cultivars, grown in greenhouse. Both genotypes showed a percentage of in vitro sprouting higher than that one previously obtained with explants collected from mature plants grown in open field. The collection time was not determinant on in vitro response
Encapsulation of cv. “Columbus” Microcuttings: A Valid Technology for hop Propagation
No full textThe spreading of hop cultivation all over the Italian territory, mainly due to the increasing interest in craft beers, led hop growers to face a big problem of nursery material supplying. Indeed, in Italy, very few are the nurseries specialized in hop plant commercialization. Traditionally, hop is propagated by cuttings, suckers, rhizomes, in vitro tissue culture, among the most recent biotechnological methods, could be both a valid alternative to traditional propagation and an efficient support for nurserymen in order to increase their hop supplying. Actually, in vitro propagation allows mass and clonal production of true-to-type plants, aseptically, in a small space and regardless of seasonality. Among tissue culture techniques, synthetic seed technology has proven to be an innovative strategy to propagate and preserve several plant species. In this study, a valid protocol for hop, cv. “Columbus”, encapsulation has been set up. As previously reported for another hop genotype, also cv. “Columbus” well responded to the encapsulation procedure, with encapsulated microcuttings maintaining their viability during the culture time; moreover, a strong influence on regrowth and conversion of 6-benzyl aminopurine concentration in the culture medium was observed. Results reported in this study represent a step forward in the application of encapsulation technology to the hop propagation, giving new insights about potentials and limitations of this technique. For these reasons, it will be necessary to carry out further studies in order to acquire better outcomes in terms of vitality and recovery for future uses in nurserie
Organogenesi in vitro da porzioni di epicotile di citrange Troyer (Citrus sinensis [L.] Osbeck x Poncirus trifoliata L. Raf.)
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Assessment of the Genetic and Phytochemical Variability of Italian Wild Hop: A Route to Biodiversity Preservation
No full textBackground: Northern Italy has an enormous heritage of hop biodiversity that need to be exploited and studied. The preservation and valorization through the characterization of the existent biodiversity is a primary goal of the European Green Deal 2023-2030. The aim of this study was to acquire information on the biodiversity of Italian wild hops. Methods: Genetic characterization of sixty accessions was done resorting to Single Sequence Repeated (SSR) markers. Phytochemical characterization of wild hops was achieved using: (i) high-performance liquid chromatography with ultraviolet detection for bitter acids quantification, (ii) steam distillation for essential oils quantification and (iii) Gas Chromatography-Mass Spectrometry for the determination of the aromatic profile. Results: The eight SSR primers showed high Polymorphic Information Content (PIC), especially HlGA23. alpha-Acids reached values between 0 and 4.125. The essential oils analysis highlighted variability within the studied population, with some accessions characterized by important spicy fraction, and others by fruity and floral notes. Conclusions: The present study allowed the characterization of Italian wild hops and demonstrated an interesting biodiversity. Part of this biodiversity have been shown to be potentially suitable for use in brewing. Moreover, several genotypes could be used in breeding programs to obtain new more sustainable varieties
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