11 research outputs found
Retracted Article: Neomycin and gentamicin detection <i>via</i> molecular recognition with cyclam-decorated gold nanoparticles
No full textWe, the Editor and Publisher of the journal Supramolecular Chemistry, have retracted the following article: Tanuja Yeluri, Rajesh S. Bhosale, Namdev V. Ghule, Aaron M. Raynor, Sidhanath V. Bhosale & Sheshanath V. Bhosale (2015) Neomycin and gentamic in detection via molecular recognition with cyclam-decorated gold nanoparticles, Supramolecular Chemistry, 27:11-12, 772-779, DOI: 10.1080/10610278.2015.1071819 Since publication, significant concerns have been raised about the integrity of data and figures in the article. When approached for an explanation, the corresponding author did not provide the requested supporting information. RMIT University, where research had been conducted, confirm they are investigating issues related to the integrity of the published work. As verifying the validity of published work is core to the integrity of the scholarly record, we are therefore retracting the article. The institution and the corresponding author have been informed. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted articles will remain online to maintain the scholarly record, but they will be digitally watermarked on each page as ‘Retracted’.</p
Rapid and sensitive High-Performance Thin-Layer Chromatographic (HPTLC) method for identification and quantification of luteolin by densitometry in Kasamarda (Cassia occidentalis L.)
No full text700-706Kasamarda (C. occidentalis L.) is a traditional herb recently recognized as a potential nutraceutical in bone health. The
current botanical nutraceutical regulations require consistent standardization for biological applications. The present study
reported the standardization of bioactive flavonoid luteolin from Cassia occidentalis L. using validated high-performance
thin-layer chromatographic (HPTLC) densitometric (DS) method. The mobile phase composition of toluene, ethyl acetate,
and formic acid was optimized to separate and identify luteolin using silica gel 60F254 aluminum plates. The densitometric
(DS) scanning was performed at 353 nm. This HPTLC-DS method was further validated as per ICH guidelines.
The linearity was 200–700 ng/band with a correlation coefficient value of 0.994. The LOD and LOQ were found to be
54.06 ng/band and 163.84 ng/band, respectively. The recovery (88.38% and 100.72%) and precision (RSD,<5%) indicated
method performance is robust and accurate for the routine analysis. Further, this bioactive flavonoid presence was confirmed
and quantified by UV-spectrumin the sample matrix using this validated HPTLC-DS method. This HPTLC-DS method was
robust, precise and accurate for quality control of active constituents present in C. occidentalis L
Delay & Cost Overrun in Construction Projects: Implementation of Lean Techniques for minimizing the risks on Time & Cost
No full textMajor challenges faced by the Construction Industry in today’s competitive market are delay & cost overruns. Many projects be in any sector energy; construction causes inefficiency in the outcomings of projects due to process or productivity reduction, quality standards & specifications, poor management practices, leading to an increase in cost & delay overruns. Many types of research in the past have researched these delay & cost overrun issues in general & propose suitable measures to overcome the issue. The research aim is to study various causes of delay & cost overrun in the construction industry & propose a strategy in minimizing risks for future projects by implementing six sigma & 5S operations.
The methodology adopted during research to validate the study is the literature review, case study, questionnaire survey. Results from each helped in finding a strategy & validating results through a mixed approach of triangulation. The research provides a strategy by integrating lean, six-sigma & 5S techniques in a project to overcome the issue of proximity of delays & cost overruns by adopting various management standards.
The strategy consists of management of quality, site, planning, procurement practices in the project. These will help in improving & managing complex construction projects in the future in avoiding risk. The findings are analyzed theoretically & statistically, to overcome the challenges. There is a need for incorporating such management practices to improve the early drawbacks of various quality standards, stakeholders’ issues, site practices in the construction projects.
The research helps in integrating & providing knowledge of various practices that can be implemented in various aspects of construction to improve construction practices & reduce significant delay & cost overruns issues. The strategy formulated by the author could help avoid & minimize risk & could be adopted in future project
LPC Models and Different Speech Enhancement Techniques- A Review
No full textAuthor has already published one review paper on the quality enhancement of a speech signal by minimizing the noise. This is a second paper of same series. In last two decades the researchers have taken continuous efforts to reduce the noise signal from the speech signal. Th is paper comments on,various study carried out and analysis propos als of the researchers for en hancement of the quality of speech signal. Various models,coding,speech quality improvement methods,speaker dependent codebooks,autocorrelation subtraction,speech restoration,producing speech at low bit rates,compression and enhancement are the vari ous aspects of speech enhancement. We have presented the review of all above mentioned technologies in this paper and also willing to examine few of the techniques in order to analyze the factors affecting them in upcoming paper of the series.
https://www.ijiert.org/paper-details?paper_id=14027
Bioanalytical Method Development and Validation Study of Neuroprotective Extract of Kashmiri Saffron Using Ultra-Fast Liquid Chromatography-Tandem Mass Spectrometry (UFLC-MS/MS): In Vivo Pharmacokinetics of Apocarotenoids and Carotenoids
No full textKashmir saffron (Crocus sativus L.), also known as Indian saffron, is an important Asian medicinal plant with protective therapeutic applications in brain health. The main bioactive in Kashmir or Indian Saffron (KCS) and its extract (CSE) are apocarotenoids picrocrocin (PIC) and safranal (SAF) with carotenoids, crocetin esters (crocins), and crocetins. The ultra-fast liquid chromatography(UFLC)- photodiode array standardization confirmed the presence of biomarkers PIC, trans-4-GG-crocin (T4C), trans-3-Gg-crocin (T3C), cis-4-GG-crocin (C4C), trans-2-gg-crocin (T2C), trans-crocetin (TCT), and SAF in CSE. This study’s objectives were to develop and validate a sensitive and rapid UFLC-tandem mass spectrometry method for PIC and SAF along T4C and TCT in rat plasma with internal standards (IS). The calibration curves were linear (R2 > 0.990), with the lower limit of quantification (LLOQ) as 10 ng/mL. The UFLC-MS/MS assay-based precision (RSD, <15%) and accuracy (RE, −11.03–9.96) on analytical quality control (QC) levels were well within the acceptance criteria with excellent recoveries (91.18–106.86%) in plasma samples. The method was applied to investigate the in vivo pharmacokinetic parameters after oral administration of 40 mg/kg CSE in the rats (n = 6). The active metabolite TCT and T4C, PIC, SAF were quantified for the first time with T3C, C4C, T2C by this validated bioanalytical method, which will be useful for preclinical/clinical trials of CSE as a potential neuroprotective dietary supplement
Rapid and sensitive High-Performance Thin-Layer Chromatographic (HPTLC) method for identification and quantification of luteolin by densitometry in Kasamarda (Cassia occidentalis L.)
No full textKasamarda (C. occidentalis L.) is a traditional herb recently recognized as a potential nutraceutical in bone health. The current botanical nutraceutical regulations require consistent standardization for biological applications. The present study reported the standardization of bioactive flavonoid luteolin from Cassia occidentalis L. using validated high-performance thin-layer chromatographic (HPTLC) densitometric (DS) method. The mobile phase composition of toluene, ethyl acetate, and formic acid was optimized to separate and identify luteolin using silica gel 60F254 aluminum plates. The densitometric (DS) scanning was performed at 353 nm. This HPTLC-DS method was further validated as per ICH guidelines. The linearity was 200–700 ng/band with a correlation coefficient value of 0.994. The LOD and LOQ were found to be 54.06 ng/band and 163.84 ng/band, respectively. The recovery (88.38% and 100.72%) and precision (RSD,<5%) indicated method performance is robust and accurate for the routine analysis. Further, this bioactive flavonoid presence was confirmed and quantified by UV-spectrumin the sample matrix using this validated HPTLC-DS method. This HPTLC-DS method was robust, precise and accurate for quality control of active constituents present in C. occidentalis L
Pharmacokinetic Study of Withanosides and Withanolides from Withania somnifera Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS)
No full textWithania somnifera is a traditional Indian herb described under the ‘Rasayana’ class in Ayurveda, which gained immense popularity as a dietary supplement in the USA, Europe, Asia, and the Indian domestic market. Despite enormous research on the pharmacological effect of withanosides and withanolides, bioanalytical method development and pharmacokinetics remained challenging and unexplored for these constituents due to isomeric and isobaric characteristics. In current research work, molecular descriptors, pharmacokinetic, and toxicity prediction (ADMET) of these constituents were performed using Molinspiration and admetSAR tools. A rapid, selective, and reproducible bioanalytical method was developed and validated for seven withanosides and withanolides as per USFDA/EMA guidelines, further applied to determine pharmacokinetic parameters of Withania somnifera root extract (WSE) constituents in male Sprague Dawley rats at a dose of 500 mg/kg. Additionally, an ex vivo permeability study was carried out to explore the absorption pattern of withanosides and withanolides from the intestinal lumen. In silico, ADMET revealed oral bioavailability of withanosides and withanolides following Lipinski’s rules of five with significant absorption from the gastrointestinal tract and the ability to cross the blood-brain barrier. Upon oral administration of WSE, Cmax was found to be 13.833 ± 3.727, 124.415 ± 64.932, 57.536 ± 7.523, and 7.283 ± 3.341 ng/mL for withanoside IV, withaferin A, 12-Deoxy-withastramonolide, and withanolide A, respectively, with Tmax of 0.750 ± 0.000, 0.250 ± 0.000, 0.291 ± 0.102, and 0.333 ± 0.129 h. Moreover, at a given dose, withanoside V, withanolide B, and withanone were detected in plasma; however, the concentration of these constituents was found below LLOQ. Thus, these four major withanoside and withanolides were quantified in plasma supported by ex vivo permeation data exhibiting a time-dependent absorption of withanosides and withanolides across the intestinal barrier. These composite findings provide insights to design a clinical trial of WSE as a potent nutraceutical
Mitotic Bookmarking Provides Epigenetic Persistence or Plasticity for Biological Control and Cancer
No full textMitotic bookmarking, the retention of regulatory proteins and lncRNAs on chromatin during mitosis, epigenetically sustains competency for phenotype-specific gene expression in progeny cells. Gene expression is predominantly suppressed during mitosis. Bookmarking provides the guidance for the resumption of gene expression in progeny cells that is obligatory for physiological control of lineage commitment, specialized cell structure and phenotypic function. While regulatory continuity is supported by the persistence of genome-associated regulatory complexes, altered bookmarking mediates plasticity for responsiveness to physiological cues. Bookmarking fidelity ensures genome integrity and controls expression of tumor suppressors and proto-oncogenes. Cancer-compromised aberrations in bookmarking results in transcriptional dysregulation and the initiation of tumor-associated processes.
Keywords: Mitotic Bookmarking, Mitosis, Epigenetic Control, Gene Expression, Cell DivisionThe presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
