2,114 research outputs found

    Development of Mass Production of VAMF Inoculum by Sand Culture

    No full text
    內生菌根菌(Vesicular-aibuscular mycorrhizal fungus, VAMF)證實與許多作物根系具有親和主,當VAMF感染作物根部形成內生菌根時,往往能產生有益效應,促進根群發育,增加根部對磷肥等要素之吸收能力,促進植株生長。惟內生菌根菌是一種活物寄生真菌,目前尚無法在文工培養基上大量繁殖,接種盆栽宿主植物繁殖內生菌根菌接種源是目前唯一可行途徑。除宿E外,土壤(栽培介質)因子、環境因子等亦可影響內生菌根菌之繁殖。本分所建立之內生菌根百接種源生產體系,分為三個步驟:(一)以多年生百喜草盆栽保存純種,(二)以盆栽玉米繁殖貝種,(三)以大型栽培床之玉米(或營多藤)大量繁殖內生菌根菌,經收集培養土(含宿主根長)風乾裝袋而成接種源。在實際應用上,採用苗期接種,培養洋香瓜內生菌根苗,當內生菌根苗移植於本田,即可將菌種帶到田間,發揮內生菌根之功能,表現其有益效應。 The vesicular-arbuscular mycorrhizal fungus was proved to have the compatibility with root systems of many crops. Formation of VA mycorrhiza in root systems of crops infected by VAM fungus could arise beneficial effects, i.e. promoted the growth and development of root systems, increased the absorption of soil mineral elements, enhanced the top growth of crops. Up today, the common method for propagation of VA mycorrhizal fungi were pot culture because they were obligate symbionts. Factors affected the propagation of VA mycorrhizal fungi in pot culture included host plants, soil (culture media), and environment. We developed a procedure for propagation of VAM fungus inoculum as follows: 1. maintaining the pure VA mycorrhizal fungus in pot culture of bahiagrass, 2. propagation of VAM fungus in pot culture of corn, 3. mass production of VA mycorrhizal fungus inoculum in sand bed culture of corn or beggarhce, 4. air-drying and bagging the harvested culture soil including the segments of infected root systems of host plants and fungal chiamydospores. Method for application of VA mycorrhizal fungus to field was by transplanting mycorrhizal seedlings of muskmelon pre-inoculated with infested soil in plastic net house. When the mycorrhizal seedlings were transplanted to the field, mycorrhizal root systems could show beneficial effects to muskmelon production

    (25(2):134-140)STUDIES ON SEED TRANSMISSION OF FLAX FUSARIUM WILT AND ITS CONTROL WITH SEED TREATMENT

    No full text
    亞麻立枯病病原菌雖係土壤傳播真菌,但也可經由種子而萬播。採收自罹病亞麻田之種子質輕、不飽滿而略扁平、表面有皺紋、無光澤、千粒重為2.3~3.0g,依發病程度而異;反之健全田之種子較重、飽滿、黑褐色、表面平滑、有光澤、千粒重為3.6g。種子攜帶病原菌之比率為0.42~6.00%,與田間亞麻罹病率成正相關。病原菌亦可侵入種皮內,其比率為0.02~0.1%。以發病亞麻田(發病率26~50%)採收之種子播種會引起萌前腐敗,亦可導致發生幼苗立枯病,前者達15.7%,後者為7.6%。 亞麻種子以50% Benlate W. P., Benlate T-20, 50%Vitavax-Thi-ram W. P., 50% Bavistin W. P.等0.5~0.1%(依重量計算)行拌種處理,可有效防止病原菌之蔓延,但因亞麻種子表面有膠質,遇水黏結成團,處理工作不易進行。故採用Dowfumo MC-2 1.5 lbs/22.32m3行燻蒸處理,為既有效又經濟之方法。 Although flax wilt pathogen, Fusarium oxysporum f. sp. lini, is a well known soil-borne fungus, it can also be transmitted by seeds. Seeds harvested from diseased flax fields are lighter, yellowish to dark brown, rough and dull, weight per thousand seeds varied from 2.3 to 3.0 g depending upon the percentage of wilt flax in the field. On the other hand, seeds from healthy flax fields are heavier, dark brown, smooth and shining, weight per thousand seeds is 3.6 g. Seeds from diseased fields carried F. oxysporum f. sp. lini with the rates varied from 0.42 to 6.00%, proportioned with the severity of flax wilt. However, wilt pathogen was detected not only on the seed but also in the seed. There were 0.02-0.10% of seeds found to carry the pathogen inside the seedcoat. Sowing of seeds from moderately diseased field (26-50% of flax wilt) resulted 15.7% of preemergence rot and 7.6% of seedling wilt. Slurry-overcoating of flax seeds with Benlate, Benlate T-20, Vitavax-Thiram, or Bavistin at the rate of 0.5-1.0% by weight was effective in controlling seedling wilt and increasing stands. However, because of flax seeds are covered with a layer of gelatinous substance and are adhering in mass when wet, slurry-overcoating is not a good method for flax seed treatment. Fumigation with Dowfume MC-2 at the rate of 1.5 lbs/22.32 cu m for 14 hr during the storage period in a compact warehouse is recommended

    (29(1):47-53)Identification of Meloidogyne arenaria on peanut in Taiwan

    No full text
    落花生根瘤線蟲病近年來在雲林地區逐漸成為重要之花生病害。其病原線蟲經鑑定為Meloidogyne arenaria ( Neal , 1889 ) Chitwocd , 1949。其成熟雌蟲之陰門模紋型(Perineal pattern)通常為卵圓型,但其長軸卻與側線(Lateral line)平行。特徵為拱弧(Arch)較低而圓,由平整條紋(Smooth striae)所構成,背部及腹部條紋(Dorsd and ventral striae)在側線處成角度之相會,側線處有許多雜亂之短條紋,肛門上方無點狀物(Punctation)。雌蟲排泄孔(Excretory pore)約位於頭端後方2 倍口針長之處。本線蟲二齡幼蟲體長1.440 mm 二-( 0.37 ~• 0.49 mm)。侵染落花生後,引起植株矮小、黃化及提早落葉等病徵。根部被感染則產生類似串珠狀之根瘤,但不像M . hapla 會自根瘤處再生放射狀之側根。豆莢及莢柄被侵害,則引起腫狀瘤而畸型。M. arenaria 對蕃茄(盛岡7 號)及小鳳西瓜也有病原性,會引起腫瘤,但對棉花(Stoneville 7A)則無病原性。 Peanut root-root-knot nematode disease has become an important and has threated to peanut production in Yien-lin growth area recently. The causal agent was identified as Meloidogyne arenaria (Neal, 1889) Chitwood, 1949. Perineal pattern of M. arenaria shows oval-shaped with longer axis in the lateral direction. Arch rounded to low is composed of smooth striae. There are numerous short disordered striae in the lateral fields where dorsal and ventral striae meet at an angle. Perineal pattern of this species is never found punctation above the anus. Female excretory pore is at about 2 stylet lengths posterior to apex of head. Median length of larvae of this species is 0.440 mm (0.37-0.49 mm). The 2nd stage larvae infect peanut, causing stunting, yellowing, and early falling of leaves. Root systems invaded by this spicies cause root knots in chains, but rarely have lateral rootlets grown directly from gall as those caused by M. hapla. Shells and stems infected by M. arenaria cause galls and become malformed. M. arenaria also causes galls and reproduces on tomato (Sheng-kang No. 7) and watermelon (Honty baby), but not on cotton (Stoneville 7A)
    corecore