250,171 research outputs found
Premier salut communiste de la Chine à la Révolution d'Octobre [Présentation, traduction et notes par J. Cheng]
No full textCheng J. Premier salut communiste de la Chine à la Révolution d'Octobre [Présentation, traduction et notes par J. Cheng]. In: Cahiers du monde russe et soviétique, vol. 3, n°3, Juillet-septembre 1962. pp. 459-474
Cheng (Vincent J.) : Joyce, Race and Empire
No full textSamin Richard. Cheng (Vincent J.) : Joyce, Race and Empire. In: Revue française d'histoire d'outre-mer, tome 83, n°313, 4e trimestre 1996. pp. 93-94
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Rebuttal from Nick J. Spencer, Tiong Cheng Sia, Simon J Brookes, Marcello Costa and Damien J. Keating
No full textMain article Crosstalk: Journal of Physiology, 2015; 593(15):3229-3231Nick J. Spencer, Tiong Cheng Sia, Simon J Brookes, Marcello Costa and Damien J. Keatin
DOP-PCR amplification of whole genomic DNA and microchip-based capillary electrophoresis.
No full textUniversal or whole genome amplification by polymerase chain reaction (PCR) is a rapid and efficient method to generate fragments representing the target sequence, as well as to increase a limited amount of template. One of the most common PCR protocols for total genome amplification is the interspersed repetitive sequence-PCR (IRS-PCR) in which primers specific for human repeat-rich regions are used to generate PCR products between adjacent repeated sequences (1). However, although IRS-PCR across regions such as Alu families of human repeat has been demonstrated to be useful, the nonuniform distribution of repeat-rich region within the human genome has been a limitation. Alternative strategies have been proposed. In the primer-extension preamplification (PEP), multiple rounds of extensions with Taq DNA polymerase and a random mixture of 15-base oligonucleotides as primers produce multiple copies of the template present in the sample (2–5). In a more demanding protocol, called linker adaptor-PCR, RsaI restricted genomic DNA fragments are ligated to SmaI-cut pUC plasmid. Subsequently, the inserts are amplified by PCR using the universal M13/pUC sequencing and reverse sequencing primers and then released by EcoRI digestion (6). The tagged random primer PCR (T-PCR) is a two-step PCR strategy which consists of a pool of all possible 3'-sequences for binding to the target DNA and a constant 5'-region for the detection of incorporated primers (7). Recently, degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) was developed to allow random amplification of DNA from any source (8–10). DOP-PCR uses a partially degenerate sequence in a PCR protocol with two different annealing temperatures. It has been successfully applied for amplifying entire genomes such as human, mouse, and fruit fly, as well as isolated human chromosomes and cosmids (11). The technique has also been used to prepare whole chromosome paint probes (11,12) for micro-FISH assays (13–15), comparative genomic hybridization (16), to increase the amount of sample for genotyping (17), and genomic fingerprinting (18). The DOP-PCR primer consists of three regions. The 5'-end carries a recognition sequence for XhoI (C•TCGAG), a restriction endonuclease that cuts rarely within the human genome. This sequence can be used for cloning, if desired. The sequence is then followed by a middle portion containing six nucleotides of degenerate sequence (NNNNNN, where N = A, C, G, or T in approximately equal proportions) and a 3'-end sequence containing six specific bases (ATGTGG) which primes the reaction approximately every 4 kb (8,9). The principle of the technique is that at a sufficiently low annealing temperature only the six specific nucleotides included in the 3'-end of the degenerate oligonucleotide will anneal to the genomic strand allowing the primer to initiate PCR. The PCR fragments are then generated which contain the full length of the oligoprimer at one end and its complementary sequence at the other end. Subsequently, the temperature is increased to the level required for the full length of the degenerate primer to anneal. For additional details, we direct the reader to the original papers (8,9). We have adapted the DOP-PCR technique to a three-microchip format (19). DOP-PCR amplified genomic DNA produced in a first silicon-glass chip is transferred to a second chip for a locus-specific, multiplex PCR of the dystrophin gene exons in order to detect deletions causing Duchenne/Becker muscular dystrophy (DMD/BMD). Amplicons from the multiplex-PCR are then analyzed by electrophoresis in a third microchip. The analytical performance of the microchip capillary electrophoresis (MCE) is also compared to conventional capillary electrophoresis (CE)
Cheng-Shiu University
No full textCerámica koji firmada por el Presidente de la Cheng-Shiu University.Nieto Nieto, J. (2022). Cheng-Shiu University. https://riunet.upv.es/handle/10251/190471DO
Graduate recital, piano. Cheng, Y.-J., 1999
No full textRecorded during a live performance at Dalton Center Recital Hall, Western Michigan University, Kalamazoo, Michigan, April 15, 1999, 5:00 p.m., the 484th concert of the School of Music's 1998-1999 season.Yuan-Jen Cheng, piano.In partial fulfillment of the requirements of the Master of Music degree in piano performance, Western Michigan University, 1999.Information from performance program.Partita no. 1 in B-flat major, BWV 825 / Johann Sebastian Bach -- Six bagatelles, op. 126 / Ludwig van Beethoven -- Intermezzo no. 1 in C major. Presto con fuoco ; Intermezzo no. 2 in D-flat major. Assez modéré mais sans lenteur ; Intermezzo in A-flat major. Très allant, tout droit, sans aucun rubata / Francis Poulenc -- Sonata reminiscenza in A minor, op. 38, no. 1: from Forgotten melodies, first cycle / Nicolas Medtner -- Songs without words. Kinderstücke, op. 102, no. 5. Allegro vivace ; Spinnerlied, op. 67, no. 4. Presto ; Duetto, op. 38, no. 6. Andante con moto / Felix Mendelssohn
The last deglaciation in Italy: timing and pattern from a precisely dated stalagmite
No full textThe last deglaciation (Termination I, T-I) was the most recent global-scale climate transition. It involved a drastic temperature increase guiding massive melting of ice sheets, with a concurrent reorganization of inter- and intrahemispherical atmospheric and ocean circulation patterns.
T-I lasted ~3.0 ka (ka = kiloyears before present) in Greenland (NGRIP, 2007), although it was not a linear process. A rapid temperature increase at 14.6±0.3 ka (Bølling-Allerød, BA) was followed by a return towards glacial-like conditions (12.2±0.3 ka, Younger Dryas, YD), before the last warming that led to the Holocene (11.7±0.1 ka). Other secondary climate oscillations characterized T-I too (Cheng et al., 2020). Some of these intra-deglaciation global warmings were particularly rapid, at times occurring at centennial or even decadal timescale. This provides an interesting comparison with the current climate change.
Yet, it is not clear how T-I-related dynamics occurring at the polar regions and/or in the oceans impacted terrestrial environment at mid latitudes, in terms of rainfall and temperature variation and related environmental and ecological changes. This is especially true for the Mediterranean area, considering that its climate is connected – and controlled – by processes occurring in the Atlantic and Arctic. In Italy, T-I records of adequate chronological resolution are virtually absent.
We here present a novel speleothem record from Sant’Angelo Cave (SA1, Ostuni, Apulia) spanning from 47.7±0.1 to 8.9±0.9 ka. In the period from ~20 to ~10 ka, multiple U-Th datings (n=22) resulted in a final age model with an average uncertainty of <0.3 ka and a resolution of ~25 years. Climate proxies (δ18O, n=1045) were anchored to this chronology. The reliability of SA1-δ18O in recording palaeoclimate information was ascertained by a statistically grounded inter-cave replication test with a recently published speleothem record from a nearby site (Columbu et al., 2020). The interpretation of SA1 allows to: 1) accurately and precisely constrain, for the first time in Italy, the timing of the T-I climate pattern; 2) evaluate the impact of BA, YD and Holocene inception in southern Italy, as well as other associated events, especially in terms of rainfall variability; and 3) understand the spatio-temporal relation between the Atlantic/Greenland domain, the Mediterranean realm and monsoonal areas throughout the deglaciation. We discuss this new record within the framework of previous regional studies based on glacial (NGRIP, 2007), marine (Martrat et al., 2007) and continental proxies (Allen et al., 1999; Cheng et al., 2016), with the aim of providing a better comprehension of the timing and structure of T-I in Italy and, by extension, of the central and western Mediterranean area.
Allen J.R.M., Brandt U., Brauer A., Hubbertens H.W., Huntley B., Keller J., Kraml M., Meckeen A., Mingram J., Negendank J.F.W., Nowaczyk N.R., Oberhansli H., Watts W.A., Wulf S. & Zolitschka B. (1999) - Rapid environmental changes in southern Europe during the last glacial period. Science, 400, 740-743.
Cheng H., Edwards R.L., Sinha A., Spötl C., Yi L., Chen S., Kelly M., Kathayat G., Wang X. & Li X. (2016) - The Asian monsoon over the past 640,000 years and ice age terminations. Nature, 534, 640.
Cheng H., Zhang H., Spötl C., Baker J., et al. (2020) - Timing and structure of the Younger Dryas event and its underlying climate dynamics. Proceedings of the National Academy of Sciences, 117, 23408-23417.
Columbu A., Chiarini V., Spötl C., Benazzi S., Hellstrom J., Cheng H. & De Waele J. (2020) - Speleothem record attests to stable environmental conditions during Neanderthal-Modern Human turnover in Southern Italy. Nature Ecology & Evolution, 4, 1188-1195.
Martrat B., Grimalt J.O., Shackleton N.J., de Abreu L., Hutterli M.A. & Stocker T.F. (2007) - Four climate cycles of recurring deep and surface water destabilizations on the Iberian margin. Science, 317, 502-507.
NGRIP, North Greenland Ice Core Project Members. (2004) - High-resolution record of Northern Hemisphere climate extending into the last interglacial period. Nature, 431, 147-151
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
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